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Nonspecific isotype control igg

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Nonspecific isotype control IgG is a laboratory reagent used to establish background signal levels in flow cytometry and immunoassay applications. It serves as a control to distinguish specific from non-specific antibody binding.

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Isotype controls (54 citations) Control IgG (22 citations) Isotype control IgG (20 citations) Isotype IgG (2 citations)

2 protocols using nonspecific isotype control igg

1

DTPA Conjugation of TRC105 Antibody

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TRC105 (TRACON Pharmaceuticals) was prepared for radiolabeling through conjugation of diethylenetriaminepentaacetic acid (DTPA, Macrocyclics) as reported previously.13 (link) The binding properties of DTPA-TRC105 have been evaluated in our previous works.13 (link) The nonspecific isotype control IgG (Invitrogen) was prepared following the same protocol.
Ehlerding E.B., Ferreira C.A., Aluicio-Sarduy E., Jiang D., Lee H.J., Theuer C.P., Engle J.W, & Cai W. (2018). 86/90Y-Based Theranostics Targeting Angiogenesis in a Murine Breast Cancer Model. Molecular pharmaceutics, 15(7), 2606-2613.
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2

Inhibition of IL-17A in Helminth Vaccine

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Monoclonal antibody was applied to the vaccinated group to induce the functional inhibition of IL-17A. Two days before and after infection, FhKTM/CpG-ODN/Coa-ASC16 vaccinated and infected mice were injected with 250 μg (100 μl i.p./mouse/dose) of αIL-17A antibody (Invitrogen, Thermo Fisher Scientific, Waltham, MA, United States). The FhKTM/CpG-ODN/Coa-ASC16 and infected groups were alternatively injected with the non-specific isotype control IgG (Invitrogen,Thermo Fisher Scientific, Waltham, MA, United States) (100 μl i.p./mouse/dose) (30 (link)).
Silvane L., Celias D.P., Romagnoli P.A., Maletto B.A., Sanchez Vallecillo M.F., Chiapello L.S., Palma S.D., Allemandi D.A., Sanabria R.E., Pruzzo C.I., Motrán C.C, & Cervi L. (2020). A Vaccine Based on Kunitz-Type Molecule Confers Protection Against Fasciola hepatica Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production. Frontiers in Immunology, 11, 2087.
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