Gcms model isq lt

Melting points were determined on Mel-Temp hot stage apparatus and were uncorrected. Reactions were routinely monitored by thin-layer chromatography on Merck silica gel PF₂₅₄ plates and spots were visualized with UV light at 254 nm. IR spectra were recorded as KBr disks on a Fourier Transform (FT-IR) Bruker spectrophotometer at Central Lab, Faculty of Science, Minia University, El-Minia, Egypt. ¹H NMR and ¹³C NMR spectra were carried out on a Bruker apparatus operating at 400 MHz and 100 MHz, respectively using TMS as internal reference at the Faculty of Science, Sohag University, Sohag, Egypt. Chemical shifts (δ values) are given in parts per million (ppm) using CDCl₃ (7.19) or DMSO-d₆ (2.5) as solvents and coupling constants (J) in Hertz. Splitting patterns are designated as follows: s, singlet; bs, broad singlet; d, doublet; t, triplet; m, multiplet. EI-MS: Mass spectrum was carried out on direct probe controller inlet part to single quadruple mass analyzer in (Thermo Scientific Gcms) Model (ISQ LT) using Thermo X-Caslibur Software at the Regonal Center for Mycology and Biotechnology (RCMB) Al-Aazhar University, Naser City, Cairo. Elemental analyses were performed on Perkin–Elmer 2400 CHN Elemental analyzer by the Microanalytical Center, Faculty of Science at the University of Cairo and are within ± 0.4% of theoretical values unless otherwise specified.

Melting points were measured in open capillary tubes on an electrothermal melting point apparatus and were uncorrected. The infrared spectra (ν, cm⁻¹) were recorded using the KBr wafer technique on a Fourier transform infrared Thermo Electron Nicolet iS10 spectrometer (Thermo Fisher Scientific Inc., Waltham, MA) at the Faculty of Science, Ain Shams University. The ¹H and ¹³C NMR spectra (δ, ppm) were measured on a Varian GEMINI (GEMINI, Manufacturing & Engineering Inc., Anaheim, CA, USA) at the Faculty of Science, Cairo University, with tetramethyl silane (TMS) as an internal standard in DMSO-d₆ as a solvent. The mass spectra were recorded on a direct probe controller inlet part to single quadrupole mass analyzer (Thermo Scientific GCMS MODEL (ISQ LT)) using the Thermo X-CALIBUR software at the Regional Center for Mycology and Biotechnology (RCMB), Al-Azhar University, Cairo, Egypt. Elemental analyses were performed on a PerkinElmer 2400 CHN elemental analyzer (PerkinElmer, Waltham, MA) at the Faculty of Science, Ain Shams University. Thin-layer chromatography (TLC) was run using TLC aluminum sheets silica gel F254 (Merck, Whitehouse Station, NJ). The 2(3H)-furanone derivative 1 and its corresponding acid hydrazide 2 were synthesized as previously reported.⁴³