Inveon multimodality pet scanner

For transportation to the imaging facility, animals were placed in their home cages in tight styrofoam boxes. Upon arrival, anesthesia was induced with 5% isoflurane in 2 L O₂/min and maintained with 2% isoflurane in 2 L O₂/min. A tail vein catheter served for radiotracer injection followed by a 70-min PET measurement (Siemens Inveon Multimodality PET scanner (Siemens, Knoxville, TN, USA) as previously described in [32 (link)]). Total anesthesia time was around 2.3 h. After being wide-awake, animals were transported back to the animal facility and were reconnected to the EEG-recording system.

¹⁸F-FDG was supplied by PETNET solutions, Nottingham, UK. Imaging was performed using the Inveon Multimodality ^™ PET scanner from Siemens Medical Solutions.
Mice received approximately 15MBq ¹⁸F-FDG administered as an i.v. bolus. Following ¹⁸F- FDG injection, anaesthesia was maintained for a 45-minute uptake period followed by a 20-minute emission PET scan. Animals were humanely euthanised following scanning. Data were acquired using Inveon Acquisition Workplace (IAW) software (Siemens) version 1.4.3 and analysed using Inveon Reconstruction Workplace (IRW) software (Siemens) version 2.2.0. The performance of the scanner has been documented previously [17 (link)]. Images were reconstructed using the order subset expectation maximisation (OSEM)/maximum a posterior (MAP) algorithm. Regions of interest (ROI’s) were drawn manually using the 3D visualisation package of IRW software. Data were expressed as the maximum standardised uptake value (MaxSUV). MaxSUV was calculated using the formula described by Gambhir et al, where ID is the injected activity [18 ].

¹⁸F-FDG was supplied by PETNET solutions, Nottingham, UK. Imaging was performed using the Inveon multimodality™ PET scanner from Siemens Medical Solutions.
Mice received approximately 15 MBq ¹⁸F-FDG administered as an i.v. bolus. The % injected dose of ¹⁸F-FDG that entered the animal was calculated using a BriTec well counter and a measurement performed before and after ¹⁸F-FDG injection. Following ¹⁸F-FDG uptake, anaesthesia was maintained for a 45-min uptake period following by a 20-min emission PET scan. Data were acquired using Inveon Acquisition Workplace (IAW) software (Siemens) version 2.2.0. The performance of the scanner has been documented previously [22 ]. Images were reconstructed using the order subset expectation maximisation (OSEM)/maximum a posterior (MAP) algorithm. Regions of interest (ROIs) were drawn manually using the 3D visualization package of IRW software. Data were expressed as the maximum standardized uptake value (SUVmax). SUVmax was calculated using the formula described by Gambhir et al. [23 ].