Elecsys kit

Manufactured by Roche

Sourced in Germany, United States

The Elecsys® kit is an in vitro diagnostic medical device used for quantitative determination of analytes in human biological samples. The kit utilizes electrochemiluminescence technology to perform immunoassay measurements.

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Cobas Elecsys estradiol and progesterone reagent kits Elecsys Progesterone II kits Elecsys IGF-I kits Elecsys FT4 III and TSH kits Elecsys Testosterone Assay Kits Roche Elecsys NT-proBNP II kits Elecsys reagent kits Elecsys HBsAg II Quant reagent kits Elecsys Cortisol II kits Elecsys GH and IGF-I kits

16 protocols using elecsys kit

Quantification of IL-6 levels

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According to the manufacturer’s protocol, IL-6 levels were determined using the Roche Elecsys Kit (09015604190). In total, 200 µL of the culture media underwent the first incubation with IL-6 specific antibodies, followed by a second incubation with IL-6 specific antibodies labeled with ruthenium complexes. After that, complexes were magnetically captured, and an electric voltage application determined the chemiluminescent emission that was directly proportional to the IL-6 concentration.

Ceccherini E., Signore G., Tedeschi L., Vozzi F., Di Giorgi N., Michelucci E., Cecchettini A, & Rocchiccioli S. (2023). Proteomic Modulation in TGF-β-Treated Cholangiocytes Induced by Curcumin Nanoparticles. International Journal of Molecular Sciences, 24(13), 10481.

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Norepinephrine and Endothelin-1 Effects on EHM

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L-Norepinephrine hydrochloride (NE) and endothelin-1 (ET-1) were prepared in distilled water containing 200 μmol/L Asc (all from Sigma-Aldrich). EHM were treated with indicated concentrations for 7 days. NT-proBNP was measured using the Elecsys^® kit (Roche Diagnostics).

Tiburcy M., Hudson J.E., Balfanz P., Schlick S., Meyer T., Liao M.L., Levent E., Raad F., Zeidler S., Wingender E., Riegler J., Wang M., Gold J.D., Kehat I., Wettwer E., Ravens U., Dierickx P., van Laake L.W., Goumans M.J., Khadjeh S., Toischer K., Hasenfuss G., Couture L.A., Unger A., Linke W.A., Araki T., Neel B., Keller G., Gepstein L., Wu J.C, & Zimmermann W.H. (2017). Defined Engineered Human Myocardium with Advanced Maturation for Applications in Heart Failure Modelling and Repair. Circulation, 135(19), 1832-1847.

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Biomarker Monitoring in Critical Care

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Blood samples will be collected and stored from all study patients, regardless of group allocation, for biomarkers measurement. Serum brain natriuretic peptide (NT-proBNP), interleukin-6 (IL-6), and neutrophil gelatinase-associated lipocalin (NGAL) will be assessed by electro-chemiluminescence “sandwich” immunoassays, using the Roche Elecsys® kit, based on polyclonal antibodies against NT-proBNP, IL-6, and NGAL, respectively. Blood samples will be collected on ICU admission, on the third, fifth, and seventh day of ICU stay, and weekly thereafter, until ICU discharge.

Rusu D.M., Siriopol I., Grigoras I., Blaj M., Ciumanghel A.I., Siriopol D., Nistor I., Onofriescu M., Sandu G., Cobzaru B., Scripcariu D.V., Diaconu O, & Covic A.C. (2019). Lung Ultrasound Guided Fluid Management Protocol for the Critically Ill Patient: study protocol for a multi-centre randomized controlled trial. Trials, 20, 236.

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Antibody Dynamics in Hybrid Immunity

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For these 4 groups of matched 50 patients each, the antibody against the RBD (Receptor Binding Domain) of the Spike protein of SARS-CoV-2 was estimated by ELISA using Elecsys kit (Roche, Switzerland) as per the manufacturer’s instructions. The kit can estimate up to a maximum of 250 IU. Whenever this threshold was reached, the sera were diluted 10 × and titers re-estimated. In such cases, the titer after dilution was taken as final. As per the manufacturers’ instructions, any titer below the cutoff of 0.8 IU was taken as negative.
Subsequently, to analyze how antibody titres behave in paired sera, we searched our cohort for those with hybrid immunity for whom paired sera were available after the first and the second doses of vaccination. We could identify 49 patients with hybrid immunity who had taken the AZD1222 vaccine and had documented COVID-19 before the first vaccine dose and serum samples were available 4–6 weeks after both doses. Since the number of hybrids after BVV152 with available paired samples was too small, we could not analyze those.

Menon A.R., Cherian S., Paul A., Kumar K., Ahmed S., Mehta P., Musthafa S., Gayathri B., Benny L, & Shenoy P. (2022). Effects of the second dose of COVID-19 vaccines in patients with autoimmune rheumatic diseases with hybrid immunity. Rheumatology International, 43(3), 449-457.

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Evaluating Serum Biomarkers in Clinical Research

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Laboratory research was carried out at the United Laboratories of Tartu University Hospital. The following biochemical parameters were assessed: serum albumin (S-Alb, g/L), serum C-reactive protein (S-CRP, mg/L), serum creatinine (S-Crea, µmol/L), serum urea (S-Urea, mmol/L), serum total calcium (S-Ca, mmol/L), serum ionized calcium (S-i-Ca, mmol/L), serum phosphate (S-Pi, mmol/L), serum uric acid (S-UA, mmol/L), serum total cholesterol (S-CHL, mmol/L), serum triglyceride (S-TG, mmol/L). Also, serum haemoglobin (S-Hb, g/L) was assessed.
Kinetic colorimetric assay for measurement of serum total alkaline phosphatase (S-tALP, normal range 35–128 iu/L) was used.
Serum 25(OH)D (S-vit D (25 OH), > 50 nmol/L) and intact parathyroid hormone (iPTH, 1.6–6.9 pmol/L) was assessed by electrochemiluminescence immunoassay and Elecsys kit (Roche) was used.
For evaluating of intact fibroblast growth factor 23 (iFGF-23, U/mL), serum was separated from peripheral venous blood samples, stored at –80 °C, and analysed by ELISA method (Millipore Corporation, Billerica, MA, USA). The normal range of iFGF-23 according to the description of the method is < 114 U/mL.

Uhlinova J., Kuudeberg A., Metsküla K., Lember M, & Rosenberg M. (2022). Significant associations between bone mineral density and vascular calcification in patients with different stages of chronic kidney disease. BMC Nephrology, 23, 327.

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Biomarkers of Stress Physiology

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Urine cortisol adjusted for creatine, and DHEA-S were assayed using a Roche Modular Analytics E170 analyzer via an Elecsys kit (Roche Diagnostics, Indianapolis, IN). The intra-assay coefficient of variance was 2.9% for cortisol and between 0.8 to 6.5% for DHEA-S. Epinephrine and norepinephrine based on 12-hour overnight urine collections adjusted for urine creatine levels, were assayed using high-pressure liquid chromatography (HPLC). The intra-assay coefficient of variation was 7.9% for epinephrine and 6.0% for norepinephrine. In regression models, cortisol, DHEA-S, epinephrine and norepinephrine were modeled as continuous variables, and were log-transformed to correct a right-skewed distribution and satisfy normality assumptions for OLS regression. We also created composite scores to capture HPA-axis burden (consisting of cortisol and DHEA-S), and the SNS burden (consisting of epinephrine and norepinephrine). For both composite measures the range was 0 to 1, which indicates the average # of high-risk indicators (i.e., in the top quartile for each of the variables within that composite score).
Further details on the methodology of the biomarkers and composite summary score creation are published elsewhere (Duncan et al., 2003 (link); Gruenewald et al., 2012 (link)).

Ransome Y., Slopen N., Karlsson O, & Williams D.R. (2017). The association between alcohol abuse and neuroendocrine system dysregulation: Race differences in a national sample. Brain, behavior, and immunity, 66, 313-321.

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Standardized Biomarker Assessment Procedures

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For assessing biomarkers, frozen blood samples were collected at the clinic visit and shipped on dry ice from the 3 GCRC cites in the U.S. and from Tokyo in Japan to a single laboratory, MIDUS Biocore Lab in Madison, WI. To ensure consistency, all samples were collected and processed using standardized procedures.
High density lipoprotein (HDL) was assessed at Meriter Labs (Madison, WI) by using a Cobas Integra® analyzer (Roche Diagnostics, Indianapolis, IN). Because the distribution of HDL in our samples was positively skewed, values were log transformed for statistical analysis.
Dehydroepiandrosterone sulfate (DHEA-S) assay was performed at the Associated Regional & University Pathologists (ARUP) laboratory (Salt Lake City, UT) with a Roche Modular Analytics E170 analyzer, using an Elecsys® kit (Roche Diagnostics, Indianapolis, IN). To reduce the effects of extreme outliers and also correct for positively skewed distribution, DHEA-S were winsorized at three standard deviations from the mean in each culture and log transformed for statistical analysis.

Yoo J., Miyamoto Y, & Ryff C.D. (2016). Positive Affect, Social Connectedness, and Healthy Biomarkers in Japan and the U.S.. Emotion (Washington, D.C.), 16(8), 1137-1146.

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Hormonal and Vitamin D Assessment

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Follicle-stimulating hormone (FSH), luteinizing hormone (LH), total testosterone (T), estradiol (E 2 ), and prolactin (PRL) were assayed with an electrochemiluminescence immunoassay (ECLIA) (Elecsys Kit; Roche Diagnostics, Germany). Serum vitamin D levels were assessed by measuring serum 25-OH-VD levels using chemiluminescence immunoassay (Liaison Assay; Diasorin, Italy). 25-OH-VD levels were divided into two categories: 25-OH-VD ≤19 ng/ml (VD deficiency) and 25-OH-VD ≥20 ng/ml (normal) [21] (link). We had no subjects with 25-OH-VD ≥50 ng/ml. Seasonal variation of VD was excluded.

Şahin B., & Alataş E. (2021). The association of vitamin D with semen quality and fertility hormones in idiopathic recurrent pregnancy loss without the female factor. Journal of Surgery and Medicine, 5(6), 611-614.

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Serum Biomarker Levels in Clinical Laboratory

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The serum samples were spare samples in the hospital laboratory reserved for biochemical purposes. All samples were stored at −80°C for future use. The levels of CA72-4, CEA, CA125 and CA19-9 were measured by an electrochemiluminescence method using Elecsys kits (CA72-4/11776258 122, CEA/11731629 322, CA125/11776223 190, CA19-9/11776193 122, Roche Diagnostics, GmhH-Mannheim, Germany). According to the kit instructions, the normal ranges of CA72-4, CEA, CA125 and CA 19-9 were 0–6.9 U/ml, 0–3.4 ng/ml, 0–35 U/ml and 0–39 U/ml, respectively. sUA and other biochemical parameters were detected by enzyme colorimetric methods. CRP was detected by a latex-enhanced turbidimetric assay using reagents and instrumentation provided by Goldsite (Shenzhen, China).

Bai X., Sun M., He Y., Liu R., Cui L., Wang C., Wan F., Wang M., Li X., Li H., Wu X, & Li C. (2020). Serum CA72-4 is specifically elevated in gout patients and predicts flares. Rheumatology (Oxford, England), 59(10), 2872-2880.

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Hyperosteogeny Tissue Analysis Protocol

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An X-ray dental panorama was performed on the oral cavity and an X-ray examination was performed on the right forearm of the proband. Pathological sections were taken from hyperosteogeny tissue of the mandibular bone, and the right tibial. Hyperosteogeny tissues were harvested and stored at formalin for further analyses. Biochemical tests were performed on the 3 ml peripheral blood obtained from the proband and his mother. After collecting peripheral blood, the serum was separated via centrifugation at 1,610 x g for 5 min at room temperature. The electrochemiluminescence immunoassay and the kits for the instrument was performed using Elecsys Cobas e 601 (Roche Diagnostics) to detect the serum levels of carcinoembryonic antigen (CEA; Elecsys CEA kit) and carbohydrate antigens including CA15-3 (Elecsys CA15-3 kit), CA19-9 (Elecsys CA19-9 kit) and CA72-4 (Elecsys CA72-4 kit). All Elecsys kits were all obtained from Roche Diagnostics GmbH.

Cai D., He F., Xu X., Xiong F, & Zhang L. (2021). APC c.4621C>T variant causing Gardner's syndrome in a Han Chinese family may be inherited through maternal mosaicism. Experimental and Therapeutic Medicine, 21(5), 488.

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