Secondary anti mouse

Manufactured by Rockland Immunochemicals

Secondary anti-mouse is a lab equipment product used to detect the presence of mouse antibodies in samples. It functions by binding to the Fc region of mouse immunoglobulins, allowing for their identification and quantification in immunoassays.

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Lab products found in correlation

Secondary anti rabbit, by Thermo Fisher Scientific (3 mentions) Odyssey imager, by LI COR (3 mentions) Protease inhibitor cocktail, by Merck Group (3 mentions) α tubulin clone b 5 1 2, by Merck Group (2 mentions) Stat3 clone k 15, by Santa Cruz Biotechnology (2 mentions) Tyrosine 705 phosphorylated stat3, by Cell Signaling Technology (1 mentions) Anti tubulin clone b5 1 2, by Merck Group (1 mentions)

3 protocols using secondary anti mouse

Western Blot Protein Extraction

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Total protein lysate was harvested in lysis buffer [150 mM sodium chloride, 1.0% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 50 mM Tris (pH 8.0)] supplemented with a protease inhibitor cocktail (Sigma, St. Louis, MO). Proteins were separated on 4%–20% SDS-PAGE gels and transferred to a polyvinylidene fluoride membrane. Antibodies against STAT3 (clone K-15; Santa Cruz Biotechnology, Dallas, TX) and α-tubulin (clone B-5-1-2; Sigma) were detected using secondary anti-mouse (Rockland, Limerick, PA) or secondary anti-rabbit (Invitrogen, Grand Island, NY) antibodies by immunoblot analysis with an Odyssey Imager (Li-COR Biosciences, Lincoln, NE).

Hogan C.H., Owens S.M., Reynoso G.V., Liao Y., Meyer T.J., Zelazowska M.A., Liu B., Li X., Grosskopf A.K., Khairallah C., Kirillov V., Reich N.C., Sheridan B.S., McBride K.M., Gewurz B.E., Hickman H.D., Forrest J.C, & Krug L.T. (2024). Multifaceted roles for STAT3 in gammaherpesvirus latency revealed through in vivo B cell knockout models. mBio, 15(2), e02998-23.

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Western Blot Analysis of STAT3

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Total protein lysate was harvested in lysis buffer (150 mM sodium chloride, 1.0% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 50 mM Tris [pH 8.0]) supplemented with a protease inhibitor cocktail (Sigma, St. Louis, MO). Proteins were separated on 4–20% SDS-PAGE gels and transferred to a polyvinylidene fluoride membrane. Antibodies against STAT3 (clone K-15; Santa Cruz Biotechnology, Dallas, TX), and α-tubulin (clone B-5-1-2; Sigma) were detected using secondary anti-mouse (Rockland, Limerick, PA) or secondary anti-rabbit (Invitrogen, Grand Island, NY) antibodies by immunoblot analysis with an Odyssey Imager (Li-COR Biosciences, Lincoln, NE).

Hogan C.H., Owens S.M., Reynoso G.V., Kirillov V., Meyer T.J., Zelazowska M.A., Liu B., Li X., Chikhalya A., Dong Q., Khairallah C., Reich N.C., Sheridan B., McBride K.M., Hearing P., Hickman H.D., Forrest J.C, & Krug L.T. (2023). B cell-intrinsic STAT3-mediated support of latency and interferon suppression during murine gammaherpesvirus 68 infection revealed through an in vivo competition model. bioRxiv.

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Immunoblot Analysis of STAT3 Activation

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Total protein lysate was harvested in lysis buffer (150 mM sodium chloride, 1.0% IGEPAL CA-630, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 50 mM Tris [pH 8.0]) supplemented with a protease inhibitor cocktail (Sigma, St. Louis, MO) and phenylmethylsulfonyl fluoride (PMSF). Proteins were separated on 10% SDS-PAGE gels and transferred to a polyvinylidene fluoride membrane. Antibodies against STAT3 (K-15; Santa Cruz Biotechnology, Dallas, TX), tyrosine 705-phosphorylated STAT3 (Cell Signaling Technology, Danvers, MA), and antitubulin (clone B-5-1-2; Sigma) were detected by the use of secondary anti-mouse (Rockland, Limerick, PA) or secondary anti-rabbit (Invitrogen, Grand Island, NY) antibodies by immunoblot analysis with an Odyssey Imager (Li-COR Biosciences, Lincoln, NE).

Reddy S.S., Foreman H.C., Sioux T.O., Park G.H., Poli V., Reich N.C, & Krug L.T. (2016). Ablation of STAT3 in the B Cell Compartment Restricts Gammaherpesvirus Latency In Vivo. mBio, 7(4), e00723-16.

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