Anti eea1 clone 14

Manufactured by BD

Anti-EEA1 clone 14 is a laboratory reagent used for the detection and localization of the EEA1 (Early Endosome Antigen 1) protein in various cell and tissue samples. It is a mouse monoclonal antibody that specifically binds to the EEA1 protein, which is a marker for early endosomes in eukaryotic cells.

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Lab products found in correlation

Anti ubiquitin clone fk2 and anti lamp1 clone ly1c6, by Enzo Life Sciences (2 mentions) Anti k48 linked polyubiquitin clone d9d5 and anti k63 linked polyubiquitin clone d7a11, by Cell Signaling Technology (2 mentions) Anti syntaxin 13 clone 151, by Synaptic Systems (2 mentions) Anti a tubulin clone b 5 1 2, by Merck Group (2 mentions) Anti ubiquitin clone p4d1, by Santa Cruz Biotechnology (2 mentions) Anti β actin clone c4, by Merck Group (2 mentions) Goat anti rabbit ig, by Thermo Fisher Scientific (1 mentions) Goat anti mouse ig, by Thermo Fisher Scientific (1 mentions) Anti lamp1 clone h4a3, by Santa Cruz Biotechnology (1 mentions) Anti ubiquitin, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Anti-EEA1 (36 citations) Clone 14 (13 citations)

4 protocols using anti eea1 clone 14

pH-sensitive GFP and AMPAR Imaging

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cDNAs encoding full-length pH-sensitive GFP, pHluorin-tagged GluA1 (pH-GluA1), and pH-GluA2 were subcloned into a vector downstream of chicken β-actin (CAG) promoter. C-terminal lysine to arginine mutants for both pH-GluA1 and pH-GluA2 were generated using the standard overlap extension PCR protocol.
Specific antibodies against GluA1 (4.9D), GluA2 (6A), GluA3 (JH4300), GluA4 (JH4303), and GFP (JH4030) were generated in the R.L.H. laboratory. The following antibodies were purchased from commercial sources: anti-ubiquitin clone P4D1 (Santa Cruz Biotechnology), anti-ubiquitin clone FK2 and anti-LAMP1 clone Ly1C6 (Enzo Lifesciences), anti-K48-linked polyubiquitin clone D9D5 and anti-K63-linked polyubiquitin clone D7A11 (Cell Signaling Technology), anti-EEA1 clone 14 (BD Transduction Laboratories), anti-syntaxin-13 clone 151.2 (Synaptic Systems), anti-a-tubulin clone B-5-1-2 (Sigma), anti-β-actin clone C4 (Millipore), and anti-PSD-95 clone K28/43 (NeuroMab). All drugs and chemicals were obtained from Tocris Biosciences or Ascent Scientific.

Widagdo J., Chai Y.J., Ridder M.C., Chau Y.Q., Johnson R.C., Sah P., Huganir R.L, & Anggono V. (2015). Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation. Cell reports, 10(5), 783-795.

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pH-sensitive GFP and AMPAR Imaging

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Characterization of mAb TS63 against CD63

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The mAb TS63 directed to CD63 has been previously described [9 (link),21 (link)]. It was produced after immunizing BALB/c mice with a mixture of HEL and Jurkat cells. Spleen cells were fused with P3 × 63AG8 mouse myeloma cells according to standard techniques, distributed into 96-well tissue culture plates, and screened by indirect immunofluorescence and immunoprecipitation. Other antibodies were an anti-GM130 (clone 35, BD transduction, IgG1), an anti-EEA1 (clone 14, BD transduction, IgG1), an anti-PDI (RL90, IgG2a, Abcam), an anti-LAMP2 (H4B4, Santa Cruz, IgG1) and an anti-CD9 (TS9, IgG1, produced in our laboratory). The goat anti mouse IgG1, IgG2a and IgG2b antibodies, coupled to Alexa Fluor 488, Alexa Fluor 568 or Alexa Fluor 647 dyes, the goat anti-rabbit Ig coupled to Dylight 650 and the goat anti-mouse Ig coupled to Alexa Fluor 680 were obtained from Thermo Fisher Scientific.

Charrin S., Palmulli R., Billard M., Clay D., Boucheix C., Van Niel G, & Rubinstein E. (2020). Rapid Isolation of Rare Isotype-Switched Hybridoma Variants: Application to the Generation of IgG2a and IgG2b MAb to CD63, a Late Endosome and Exosome Marker. Antibodies, 9(3), 29.

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Immunostaining of Subcellular Markers

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Cells were immunostained as described previously (Hirota et al., 2004) . The following antibodies were used in this study: anti-AP-1 (clone 100/3, Sigma), anti-EEA1 (clone 14, BD Transduction Laboratories), anti-LAMP-1 (clone H4A3, Santa Cruz), anti-LC3 (MBL, #PM036), anti-p62 (MBL, #PM045), antiubiquitin (Dako, Z045801), and Alexa Fluor 589-labeled secondary antibodies (Molecular Probes). Immunofluorescence observations were performed using a laser-scanning fluorescence microscope (FV1000; Olympus) equipped with diode-pumped solid-state lasers (473 and 559 nm).

Tsutsui H., Jinno Y., Shoda K., Tomita A., Matsuda M., Yamashita E., Katayama H., Nakagawa A, & Miyawaki A. (2015). A diffraction-quality protein crystal processed as an autophagic cargo. Molecular cell, 58(1).

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