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2 protocols using ha14 1

1

Modulating Autophagy and MAPK in Embryos

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st24 embryos were dechorionated and incubated for 24 h in 50 nM Bafilomycin A (Sigma), 25 μM PD98059 (Cell Signaling), or 6 μM HA14‐1 (Sigma). Drugs were diluted in 3% DMSO/embryo medium. Medium was refreshed twice a day. Control embryos were grown in 3% DMSO/embryo medium.
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2

Prostaglandin D2 Receptor Agonists and Antagonists

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Prostaglandin D2 receptor agonists PGD2, BW245c, 13,14‐di‐hydro 15‐keto PGD2 (DK‐PGD2), and antagonists MK0524, Cay10471, and BW A868c were purchased from Cayman, Ann Arbor, USA, and rosiglitazone, BH3I‐1, and HA14.1 were purchased from Sigma–Aldrich, St. Louis, USA.
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