Dodecane

To determine the retention indices (RI), the following hydrocarbons were used: nonane (≥99%), decane (≥99%), undecane (≥99%), dodecane (99%), tridecane (≥99%), tetradecane (≥99%), and hexadecane (≥99%) purchased from Merck KGaA (Darmstadt, Germany). Dichloromethane (Sigma Aldrich, Steinheim, Germany) was used for the dilution of the EO.

Cysteamine (CYA) was purchased from TCI (>98.0%, Tokyo, Japan). L-α-phosphatidylcholine, mucin from a porcine stomach (Type II), sodium dodecyl sulphate, phosphate buffer (PBS, pH 7.4), as well as components for simulated tear fluid (STF) were purchased from Sigma-Aldrich/Merck (Budapest, Hungary). The analytical grade solvents including methanol, acetonitrile, hexane, dodecane, and chloroform were purchased from Merck KGaA (Darmstadt, Germany). A sample of 0.55% viscous CYA eye drop (Cystadrops^®, 3.8 mg/mL cysteamine hydrochloride) was obtained from Recordati Rare Diseases, (Puteaux, France).

For the determination of the retention indices (RI) of the separated compounds, the following hydrocarbons were used: nonane (≥99%), decane (≥99%), undecane (≥99%), dodecane (99%), tridecane (≥99%), tetradecane (≥99%), and hexadecane (≥99%) purchased from Merck KGaA (Darmstadt, Germany). Hexane (Thermo Fisher Scientific GmbH, Bremen, Germany) was used for the dilution of the EO.

The following hydrocarbons were used to determine the retention indices (RIs): nonane (99%), decane (≥99%), undecane (≥99%), dodecane (99%), tridecane (≥99%), tetradecane (≥99%), hexadecane (≥99%), heptadecane (99%), octadecane (99%), nonadecane (99%), eicosane (99%), heneicosane (≥99.5%), tricosane (99%), tetracosane (99%), pentacosane (99%), octacosane (99%), and triacontane (99%), and they were purchased from Merck KGaA (Darmstadt, Germany). GC-grade hexane was used for diluting the essential oils, purchased from Thermo Fisher Scientific GmbH (Bremen, Germany). Fluorescein (3′,6′-dihydroxy-3H-spiro [2-benzofuran-1,9′-xanthen]-3-one) (CAS 2321-07-5), AAPH (2,2′-azobis-(2-methylpropionamidine)-dihydrochloride) (CAS 2997-92-4), and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) (CAS 53188-07-1) were purchased from Sigma-Aldrich (Darmstadt, Germany).

L-lactide, Candida rugosa lipases (CRL) (catalogue #62316), N,N-dimethylacetamide (DMA), 1,2-dimethoxybenzene, 1,4-dimethoxybenzene, diphenyl ether, and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF₆]) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dodecane, chloroform, and methanol were obtained from Merck KGaA (Darmstadt, Germany). The solvents used were of ≥99% purity, while chloroform and methanol were of EMSURE grade.

For determination of retention indices (RIs), heptane (99%), octane (≥99%), nonane (99%), decane (≥99%), undecane (≥99%), dodecane (99%), tridecane (≥99%), tetradecane (≥99%), hexadecane (≥99%), heptadecane (99%), octadecane (99%), nonadecane (99%), and eicosane (99%) purchased from Merck KGaA (Darmstadt, Germany) were used. For diluting essential oils, hexane purchased from Thermo Fisher Scientific GmbH (Bremen, Germany) was used.

All
chemicals were used
directly as provided commercially without additional purification.
Milli Q water was obtained using an IQ 7000 purifying system. P25
TiO₂, HAuCl₄·3H₂O, PdCl₂, acetone (99.9%, CH₃COCH₃), sodium
hydroxide (reagent grade, 97%, powder, NaOH), 1-phenylethanol (97%),
hexanoic acid (99%), pentane (99%), lauric acid (97%), palmitic acid
(99%), stearic acid (98%), oleic acid (97%), undecane (99%), pentadecane
(99%), heptadecane (99%), p-xylene (99.5%, as internal
GC standard), different solvents including dodecane (99%), PhMe (99%),
THF (99%), DMF (99%) and dichloroethane (99%) were purchased from
Sigma-Aldrich. Jatropha oil was purchased from the Shenyu company
in Yunnan Province, China. Wasted cooking oil and wasted hot-pot oil
were collected from Sichuan Province, China.