The sections were stained with hematoxylin and eosin (HE), Movat's pentachrome16 (link)), or Masson trichrome17 (link)). HE staining was used to determine the size of the necrotic core. Immunostaining was performed as described previously9 (link), 18 (link)). In brief, the sections were incubated with the primary antibody against mouse MOMA-2 (1:600; Bio-Rad) or α-SMA (1:100; abcam) overnight at 4°C. After washing, the sections were incubated with biotinylated anti-rat antibody (1:200; Vector Labs) or anti-rabbit antibody (1:200; Vector Labs) for 2 h at 37°C, and then with avidin-biotin peroxidase complex (Vector Labs) for 30 min. Last, the sections were developed with 3, 3'-diaminobenzidine tetrahydrochloride (DAB) (Sigma), and counterstained with hematoxylin.
Biotinylated anti rat antibody
Manufactured by Vector Laboratories
Sourced in United States
Biotinylated anti-rat antibody is a laboratory reagent used to detect the presence of rat-specific proteins or target molecules in various biological samples. It is a secondary antibody conjugated with biotin, which can be used in a variety of immunoassay techniques, such as ELISA, Western blotting, and immunohistochemistry, to amplify the signal and enhance the sensitivity of the detection process.
Automatically generated - may contain errors
Lab products found in correlation
α sma, by Abcam (1 mentions)
3 3 diaminobenzidine tetrahydrochloride dab, by Merck Group (1 mentions)
Avidin biotin peroxidase complex, by Vector Laboratories (1 mentions)
Vectastain elite abc reagent, by Vector Laboratories (1 mentions)
Vectastain elite abc, by Vector Laboratories (1 mentions)
Anti f4 80 antibody, by Bio-Rad (1 mentions)
4 protocols using biotinylated anti rat antibody
1
Histological Analysis of Atherosclerotic Lesions
2
Assessing Cerebral IgG Extravasation
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Ten μm sections were stained with hematoxylin and eosin (H&E) for examination of ischemic and haemorrhagic lesions. Additional 10 μm sections were immunostained for IgG to assess cerebral IgG extravasation as an index of blood brain barrier breakdown. For this reason, deparaffinized sections were soaked in 3% hydrogen peroxide to block endogenous peroxidase activity and were incubated for 1 hr with a biotinylated anti-rat antibody (1:200; Vector Laboratories, Burlingame, CA). The immunoreaction was visualized with diaminobenzidine. The cerebral vessels showing IgG extravasation density (number for mm2) was quantified in 20 randomly selected fields spanning the frontal cortex.
3
Immunohistochemical Analysis of α7-nAChR in Lung Tissue
4
Macrophage Detection in Islet Grafts
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Immunohistochemistry was performed as described previously 16 . Kidneys carrying islet grafts were snap frozen in precooled 2-methylbutanolin liquid nitrogen. Sections of 5-μm size were fixed in cold acetone for 3 min, followed by immunohistochemistry with anti-F4/80 antibody (1:1,000; Serotec, Oxford, UK) to detect the presence of macrophages in the transplanted islets. The secondary antibody was biotinylated anti-rat antibody (1:1,000; Vector Laboratories, Burlingame, CA, USA). Slides were observed under light microscopy.
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