Prism 6 for windows

Al data are presented as means and the error bars indicate the standard deviations. The results from the flow cytometry and the quantification of the results from the Western blots were analyzed using ANOVA and post-hoc Dunnett’s multiple comparisons tests. The analysis was performed using Prism 6 for Windows (GraphPad Software Inc., La Jolla, CA, USA).

The data for Saline and LPS groups were expressed as a fold change of the mean ± SEM to the mean of the Saline group. These data were analysed by using Prism 6 for Windows (Version 6.01, GraphPad Software, Inc., La Jolla, CA, USA). The data were analysed using two-tailed unpaired parametric Student’s t-test. All differences were considered to be significant at p < 0.05.

Statistical analyses were performed using Prism 6 for Windows (Graph Pad) and comparisons between groups were performed using the Wilcoxon rank-sum statistic (Mann-Whitney). Correlations were calculated using the Spearman’s rank correlation. The multiple regression analyses were performed with SAS (version 9.3, SAS Institute).

The nonlinear regression was performed using “Prism 6 for Windows” software (version 6.03, GraphPad Software Inc., CA, USA) in order to assume that the R_CA and R_MCA (%) follow the Gaussian distribution.
The one-way analysis of variance (ANOVA) was executed using DSTAT meta-analytic software [20 ]. The data obtained were analyzed for statistical significance for testing the: (i) effects of the single factors (extraction rate) on the MCA; (ii) significant differences between the curd yield parameters (CY_F, CY_S and CY_W), at each temperature incubation, considering the independent factors (coagulant and milk types). Tukey’s comparison was used to verify the significance between groups. A p-value ≤ 0.05 was considered significant.
The results are reported as the means ± standard deviations.

Lineal regression equations for the GM of MDA titers versus time were calculated. The normal distribution of the data was assessed with the Kolmogorov–Smirnov and D’Agostino–Pearson tests. The Kruskal–Wallis test was used to compare antibody titers among the six groups of animals and Dunn’s multiple comparison test was used to look for individual differences among groups. The Mann–Whitney test was applied to compare antibody titers between two groups. The statistical package GraphPad Prism 6 was used for the entire analysis (Prism 6 for Windows, Version 6.01, GraphPad Software, Inc., La Jolla, USA). Statistical significance was considered when p < 0.05.