Soy protein isolate (SPI), with 90% protein on a dry basis, was supplied by ADM Protein Specialties Division (Amsterdam, The Netherlands); SPI has 5% moisture, 4% fat, and 5% ash and its isoelectric point is 4.6 [17 (link)]. Polyvinyl alcohol (PVA) (89,000–98,000 molecular weight, 99% hydrolyzed) and phosphate buffered saline (PBS) tablets were purchased from Sigma-Aldrich (Madrid, Spain). Porcine gelatin (GEL), with a bloom of 270, was supplied by Sancho de Borja (Borja, Spain). Sodium alginate (SA), with a viscosity of 350–550 mPa·s (1%, 20 °C), and glycerol (Gly), used as plasticizer, were supplied by PanReac (Barcelona, Spain).
Sodium alginate
Manufactured by ITW Reagents
Sourced in Germany, Spain
Sodium alginate is a water-soluble polysaccharide extracted from brown seaweed. It is a common ingredient used in various laboratory applications due to its gelling and thickening properties.
Automatically generated - may contain errors
Lab products found in correlation
Calcium chloride, by Merck Group (4 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Glycerol, by ITW Reagents (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Zinc chloride, by Merck Group (1 mentions)
Hyclone, by GE Healthcare (1 mentions)
Gelatin, by Merck Group (1 mentions)
Graphene oxide nanosheets, by Merck Group (1 mentions)
N 1 naphthyl ethylenediamine dihydrochloride, by Merck Group (1 mentions)
Millex gp filter unit, by Merck Group (1 mentions)
Hydrogen peroxide solution, by Merck Group (1 mentions)
Calcium chloride dihydrate, by Merck Group (1 mentions)
Amplex red reagent, by Thermo Fisher Scientific (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Sulfanilamide, by Merck Group (1 mentions)
Sodium dihydrogen phosphate dihydrate, by Merck Group (1 mentions)
Disodium hydrogen phosphate dodecahydrate, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Phosphoric acid, by ITW Reagents (1 mentions)
7 protocols using sodium alginate
1
Soy Protein Isolate-based Biomaterials
2
Antimicrobial and Cytotoxic Evaluation of Carbon Nanofibers
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Calcium chloride (≥93.0%) and zinc chloride (≥97.0%) from Sigma-Aldrich (Buchs, Switzerland), carbon nanofibers (CNFs, Graphenano, Yecla, Spain) and sodium alginate (Panreac AppliChem, Darmstadt, Germany) were used as received. Tryptic soy broth (TSB) and tryptic soy agar (TSA) from Liofilchem (Teramo, Italy), and the Gram-positive multidrug-resistant MRSE, RP62A [45 (link)], was used in the antibacterial tests. Non-tumorigenic immortalized human keratinocyte HaCaT cells were supplied by the Medical Research Institute Hospital La Fe, Spain. DMEM medium containing 10% FBS (Biowest SAS, Nuaillé, France), penicillin (Lonza, Verviers, Belgium) and streptomycin (HyClone, GE Healthcare Life Sciences, Logan, UT, USA), dimethyl sulfoxide (DMSO, Sigma Aldrich, Buchs, Switzerland) were used in the cytotoxicity tests. Isotonic saline solution (NaCl 0.9% w/v) from FisioVet (B. Braun VetCare SA, Barcelona, Spain) was used in the antibacterial and cytotoxicity tests of the CNFs in pure form.
3
Gelatin and Sodium Alginate Preparation
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All chemicals were used without additional purification. Gelatin was purchased from Sigma-Aldrich and was type A from porcine skin (roughly 300 g Bloom). Sodium alginate was purchased from PanReac AppliChem and was of medium viscosity (350 to 550 mPa s at 1%), with a molecular weight of 10,000 to 600,000 g/mol.
4
Sodium Alginate Characterization and Application
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The sodium alginate (Panreac AppliChem, Darmstadt, Germany) was analysed by 1H-NMR and Gel Permeation Chromatography (GPC). The results of this characterisation showed an average molar mass of 142.000 g/mol and a mannuronate/guluronate ratio of 1.56. Calcium chloride ( 93.0%, Sigma-Aldrich, Saint Louis, MO, USA) and CNFs (Graphenano, Yecla, Spain) were utilised as received.
5
Sodium Alginate Graphene Oxide Hydrogel
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Sodium alginate (Panreac AppliChem GmbH, Darmstadt, Germany), calcium chloride as crosslinker (≥93.0%, Sigma-Aldrich, St. Louis, MO, USA), graphene oxide nanosheets (Product Code: 796034, 15–20 sheets, 4% to 10% edge-oxidized, Sigma-Aldrich, USA) and carbon nanofibers (Product Code: 13/0248, Graphenano, Yecla, Spain) were utilized as supplied.
6
Spectrophotometric Determination of Antioxidants
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Sodium alginate ((C6H7O6Na)n, 99.8%, MW = 10–600 kDa, viscosity at 1% and 20 °C = 450 mPa) and phosphoric acid (H3PO4, 85.0–88.0%w/w) were purchased from Panreac. Sodium glucoronate monohydrate (C6H9NaO7·H2O, 97.5%), sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O, >98.0%), disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O, >98.0%), sulfanilamide (C6H8N2O2S, ≥99%), calcium chloride dihydrate (CaCl2·2H2O, >96.0%), N-(1-naphthyl)ethylenediamine dihydrochloride (C12H16Cl2N2, >98%), horseradish peroxidase Type VI (HRP), hydrogen peroxide solution (H2O2, 30%w/w), sodium nitrite (NaNO2, >98.0%), disodium terephthalate (C6H4(COONa)2, 96%) and 2-hydroxyterephtalic acid (C8H6O5, >97%) were purchased from Sigma Aldrich. Formic acid (HCOOH, 98–100%) was purchased from Supelco. Amplex Red reagent was purchased from Thermo Fisher Scientific. Acetonitrile (ACN, 99.9%) was purchased from Carlo Erba Reagents. Ultrapure water was obtained by filtration using 0.22 μm pore size MILLEXGP filter unit (Merck Millipore). Helium gas (99.998%, maximum impurities of O2 and H2O 5 ppmv) was provided by Praxair.
7
Sodium Alginate Characterization and Crosslinking
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Sodium alginate was purchased from Panreac AppliChem (Germany) and was used as received. This Sodium alginate was characterized by 1H-NMR and Gel Permeation Chromatography (GPC). Thus, the mannuronate/guluronate ratio was 1,56 and the average molar mass was 142.000 g/mol. Calcium chloride ( 93.0%, Sigma-Aldrich) as crosslinker and graphene oxide powder (Sigma-Aldrich) were used without further purification.
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