Dulbecco s modified eagle s medium
Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium that provides essential nutrients and growth factors for the cultivation of various cell types in vitro. It is a widely used basal medium for supporting the growth and maintenance of a diverse range of mammalian cell lines.
Lab products found in correlation
188 protocols using dulbecco s modified eagle s medium
Glucose Deprivation Effects on HEK293 and HFF-1 Cells
Assessing Cellular Viability and Oxidative Stress
MERS-CoV Infection in hDPP4-Tg Mice
Cell Culture Protocols for Cancer Research
Generating Bone Marrow-Derived Macrophages
Bone marrow-derived macrophages (BMDMs) were cultured in Dulbecco’s modified Eagle’s medium (Welgene Co., Daegu, Korea) supplemented with 10% fetal bovine serum (FBS) (Welgene), 100 unit/ml penicillin/100 μg/ml streptomycin (Welgene) and 50 ng/ml mouse macrophage colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA) at 37°C and 5% CO2 for 6 days.
Melanoma and Keratinocyte Cell Culture
A non-tumoral human epithelial cell line, HaCaT cells, was generously provided by Professor Jong Kun Park at Wonkwang University (Iksan, Republic of Korea). HaCaT cells were cultured in RPMI-1640 medium (WelGene, Gyeongsan, Republic of Korea) supplemented with 10% FBS and 100 U/mL PS at 37 °C and 5% CO2 in a humidified incubator until utilized.
Ovarian Cancer Cell Line Classification
Silencing CTCF in Human Extravillous Trophoblast Cells
Osteoclast Differentiation Assay
Eagle’s medium (α-MEM), penicillin-streptomycin solution, and
fetal bovine serum (FBS) were purchased from Welgene (Gyeongsan, Korea). TaqMan
Gene Expression Master Mix, TaqMan probes (5’-fluorescein based reporter
dye; 3’-TAMRA quencher), and High-Capacity RNA-to-cDNA Kit were purchased
from Applied Biosystems (Foster City, CA, USA). RANKL was purchased from
purchased ProSpec (Rehovot, Israel). All other reagents used in the experiment
were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Culturing A549 and Plat-A Cells
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