Nanoscope 9
The Nanoscope 9.2 is a high-resolution atomic force microscope (AFM) designed for advanced surface characterization. It provides precise nanoscale imaging and measurement capabilities, enabling users to visualize and analyze the topography and properties of various samples.
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10 protocols using nanoscope 9
Optimized AFM Protocol for Cell Mechanics
Atomic Force Microscopy of Microgels
AFM Imaging for Cytoskeleton Analysis
The large amount of images necessary to train and test the CNN model required high image throughput. This was achieved with a fast-scan AFM system, using photothermal off-resonance tapping and small cantilevers, which fits on the base of the commercial MultiMode AFM system. The fast scanning AFM head and electronics were designed by Georg E. Fantner’s group, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland98 (link), images were collected with custom software from the group of Georg Fantner, EPFL, (IbniAFMController-BetaVersionv2.0.16-20190117 written under Labview environment). Scans were made with a silicon tip on a silicon nitride cantilever- (Bruker FASTSCAN-B). All cytoskeleton images were analyzed using Gwyddion 2.55 software99 .
Atomic Force Microscopy Nanoindentation Protocol
Nanomechanical Characterization of Biological Fibrils
Hydrogel Stiffness Measurement by AFM
Atomic Force Microscopy and Mass Spectrometry
Nanomechanical Characterization of Multilayered Films
Comprehensive Characterization of Cellulose Nanofibrils
measurements, a DV2TLV viscometer (Brookfield) was used along with
Rheocalc software. Each CNF sample (0.4% CNF suspension) was evaluated
in three parallels, and the viscosity of each parallel was measured
from 0.1 to 100 rpm. For the nanostructures, 0.01% nanocellulose suspensions
of each sample (25 μL) was added to clean the mica surface,
left to dry in air, and then imaged with a Dimension ICON atomic force
microscope (AFM) using NanoScope 9.4 Software (Bruker). Surface roughness
(Ra) was obtained from images (10 μm
× 10 μm) using NanoScope Analysis software (version 1.9).
For the quantification of residual fiber content, a Fiber Tester
912 Plus (ABB AB/Lorentzen Wettre) was used. CNF suspensions were
pumped through a flow cell where the fibers were photographed with
a resolution of 4 μm. The images were further used to analyze
the mean length and width of the fibers. To evaluate the microstructure
of all cellulosic materials, tissue culture plates (24-well clear
flat bottom polystyrene; NUNC, Denmark) were covered with cellulosic
suspensions and stored at 37 °C for 24 h to dry. Films were analyzed
using an optical microscope (Nikon Eclipse 80i, Tokyo, Japan) after
staining with crystal violet.
Adsorption of Emulsion Droplets on Mica Substrate
The emulsion droplet was incubated on the mica substrate surface for 10 min. Then, the substrate was washed with 1 mL of deionized water, which was obtained using a Simplicity UV system (Millipore, Molsheim, France). The washed substrate was dried in air and subjected to AFM scanning.
The AFM images of nanosized particles were obtained with a Dimension atomic force microscope equipped with an Icon™ scanner (Bruker, Billerica, MA, USA). The instrument is a part of the Avogadro unique research facility (
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