Titanium (IV) nitrate tetrahydrate (Ti(NO3)4.H2O, ≥99.9%), pure grade of polyethylene glycol (PEG), analytical-grade acetone (anhydrous, ≥99.0%), ethanol (96%), 3% of glutaraldehyde in phosphate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) rhodamine 123 dye, acridine orange/ethidium bromide (AO/EtBr) dye, dichloro dihydro fluorescein diacetate (DCFH-DA), and anhydrous sodium sulfate were acquired from Sigma-Aldrich ( Hamburg, Germany). Ciprofloxacin (5 μg/disc, MASTDISCS™, Mast Diagnostics Ltd.), vancomycin (Glaxo SmithKline Pharmaceuticals Ltd.), and gentamycin (Alpha Laboratories Ltd.) were purchased from local drug stores.
Analytical grade acetone
Manufactured by Merck Group
Sourced in Germany
Analytical grade acetone is a high-purity solvent used in various laboratory applications. It is a colorless, volatile liquid with a characteristic odor. Acetone is commonly used as a cleaning agent, a reagent in chemical reactions, and a solvent for a wide range of organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Petroleum ether, by Merck Group (2 mentions)
Folin ciocalteu reagent, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Anhydrous sodium sulfate, by Merck Group (1 mentions)
Dichloro dihydro fluorescein diacetate dcfh da, by Merck Group (1 mentions)
Vancomycin, by GlaxoSmithKline (1 mentions)
Cellulose acetate, by Merck Group (1 mentions)
Pure ethyl alcohol, by Merck Group (1 mentions)
Pluronic f 127, by Merck Group (1 mentions)
Astaxanthin, by Merck Group (1 mentions)
Sequencing grade modified trypsin, by Promega (1 mentions)
Bca protein assay, by Thermo Fisher Scientific (1 mentions)
Protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions)
Teab solution, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Dextran sulfate sodium (dss), by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Acetonitrile hypergrade, by Merck Group (1 mentions)
14 protocols using analytical grade acetone
1
Synthesis and Characterization of Titanium Nitrate Polymer Composites
2
Manufacture and Sterilization of Cellulose Acetate-based Nerve Guidance Conduits
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Cellulose acetate (CA) (Product Code: 419028, ~50 g/mol, Sigma-Aldrich, Saint Louis, MO, USA) and soy protein acid hydrolysate (SPAH) (Product Code: S1674, Sigma-Aldrich, Saint Louis, MO, USA) were used to manufacture the NGCs. Analytical grade acetone (Product Code: 270725 purity 99.9%, 58.08 g/mol, Sigma-Aldrich, Saint Louis, MO, USA) was used to dissolve the CA and SPAH.
Prior to use, the CA was sterilized in 0.5 g aliquots in 2 mL cryogenic tubes (Sorenson BioScience, Salt Lake City, UT, USA). Before use, the SPAH was subjected to ultraviolet radiation for 30 min inside the laminar flow cabinet of a vertical air blower (BBS-v800, Biobase, Tsinan, China).
The antisepsis of the work area was carried out with ethyl alcohol 70° (Pure Ethyl Alcohol, Sigma-Aldrich, Saint Louis, MO, USA). The necessary instruments were as follows: anatomical forceps, scalpel handle No. 3, disposable scalpel blade No. 15, 1.4 mm diameter galvanized wire, 6 cm straight scissors, disposable sterile field cloths (square area of 0.25 m2) and sterile gauze (square area of 0.0016 m2). The asepsis of the instruments and aliquots of CA was carried out in a 12-L semiautomatic autoclave (Dabi-Atlante, Ribeirão Preto, Brazil).
Prior to use, the CA was sterilized in 0.5 g aliquots in 2 mL cryogenic tubes (Sorenson BioScience, Salt Lake City, UT, USA). Before use, the SPAH was subjected to ultraviolet radiation for 30 min inside the laminar flow cabinet of a vertical air blower (BBS-v800, Biobase, Tsinan, China).
The antisepsis of the work area was carried out with ethyl alcohol 70° (Pure Ethyl Alcohol, Sigma-Aldrich, Saint Louis, MO, USA). The necessary instruments were as follows: anatomical forceps, scalpel handle No. 3, disposable scalpel blade No. 15, 1.4 mm diameter galvanized wire, 6 cm straight scissors, disposable sterile field cloths (square area of 0.25 m2) and sterile gauze (square area of 0.0016 m2). The asepsis of the instruments and aliquots of CA was carried out in a 12-L semiautomatic autoclave (Dabi-Atlante, Ribeirão Preto, Brazil).
3
Fluorescent AuQD-Based Optoelectronic Devices
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Three types of AuQDs (<2 nm) with different fluorescence emission wavelengths – blue-AuQDs (mixed Au5 and Au8; 5 and 8 Au atoms), green-AuQDs (Au13; 13 Au atoms), and red-AuQDs (Au25; 25 Au atoms) (B-AuQDs, G-AuQDs, and R-AuQDs, respectively) – were purchased from Dai Nippon Toryo Co. Ltd. (Japan). All of them were capped by pepsin molecules as a stabilizer.21 (link) Poly(3-hexylthiophene-2,5-diyl) (P3HT), [6,6]-phenyl C61 butyric acid methyl ester (PCBM), 1,2-dichlorobenzene, and a colloidal solution of AuNPs with an average diameter of 5 nm were purchased from Sigma-Aldrich (Japan). An indium tin oxide (ITO)-coated glass substrate with a conductivity of 10 Ω cm−2 was purchased from Furuuchi Chemical (Japan). Concentrated hydrochloric acid (HCl, 37%) and analytical grade acetone were purchased from Sigma-Aldrich (Japan).
4
Astaxanthin-loaded PLGA Nanoparticles
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Astaxanthin was purchased from Sigma-Aldrich Chemical (St. Louis, MO). PLGA with a molecular weight ranging from 7,000 to 240,000 kDa (lactide:glycolide 50:50) was supplied by Boehringer Ingelheim (Ingelheim, Germany). The surfactant Pluronic F-127 and analytical grade acetone were procured from Sigma-Aldrich Chemical and Merck (Darmstadt, Germany), respectively.
5
Breast Tissue Proteome Preparation
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Minced breast tissues were grinded in liquid nitrogen with a mortar and pestle, lysed in 2% SDS-lysis buffer (pH 8.0) containing protease and phosphatase inhibitors (Thermo Fisher Scientific, USA), then sonicated for 3 min (5 sec on and 5 sec off, amplitude 25%). The lysate was centrifuged at 16,000g for 30 min, and the supernatant was collected as whole-tissue extract. Protein concentration was determined using the BCA protein assay (Thermo Fisher Scientific).
Each aliquot of 100 μg of breast proteins was diluted to 100 μl with 100 mM TEAB solution (Sigma-Aldrich, USA). Each sample was reduced with 10 mM TCEP for 30 min at 56 °C, followed by alkylation with 20 mM iodoacetamide for 30 min in the dark at room temperature. Then, proteins were precipitated overnight at -20 °C using analytical grade acetone (Sigma-Aldrich). The recovered proteins after pelleting by centrifugation at 8,000g for 5 min were resuspended with 100 μl 100 mM TEAB solution. Sequencing-grade rLys-C (Promega, USA) was added to a final protease:protein ratio of 1:100 (w/w) and incubated for 3 h at 37°C. Samples were then digested by sequencing-grade modified trypsin (Promega) at ratio of 1:50 (w/w) for 16 h at 37°C. Peptides were collected by centrifugation at 16,000 g for 20 min.
Each aliquot of 100 μg of breast proteins was diluted to 100 μl with 100 mM TEAB solution (Sigma-Aldrich, USA). Each sample was reduced with 10 mM TCEP for 30 min at 56 °C, followed by alkylation with 20 mM iodoacetamide for 30 min in the dark at room temperature. Then, proteins were precipitated overnight at -20 °C using analytical grade acetone (Sigma-Aldrich). The recovered proteins after pelleting by centrifugation at 8,000g for 5 min were resuspended with 100 μl 100 mM TEAB solution. Sequencing-grade rLys-C (Promega, USA) was added to a final protease:protein ratio of 1:100 (w/w) and incubated for 3 h at 37°C. Samples were then digested by sequencing-grade modified trypsin (Promega) at ratio of 1:50 (w/w) for 16 h at 37°C. Peptides were collected by centrifugation at 16,000 g for 20 min.
6
Analytical Standards of Bisphenol Compounds
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Analytical standards of BP-8, BP-1, BP-3 and 4-OH-BP; MSTFA and β-glucuronidase enzyme were provided from Sigma Aldridge (St. Louis, MO, USA). Analytical grade acetone, methanol and trichloromethane as dispersing and extraction solvents were purchased from Merck (Darmstadt, Germany). Stock solutions of the target compounds were prepared in methanol and stored in a dark vial at 4 °C until use. For preparing working standard solutions, the stock solution was diluted in synthetic urine and used for GC/MS calibration. An Agilent technology GC/ triple quadrupole MS was used for the quantification of the BP metabolites.
7
Extraction and Analysis of Spirulina
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Spirulina platensis powder was obtained from Recon Healthcare (Bangalore, India). Analytical grade acetone, hexane, and ethyl acetate and HPLC grade methanol were purchased from Merck (Darmstadt, Germany). DSS (molecular weight 40000) was purchased from Sigma Aldrich (St. Louis, MO, USA).
8
Astaxanthin Detection and Quantification
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Analytical-grade acetone, petroleum ether and hypergrade acetonitrile were obtained from Merck (Darmstadt, Germany). Ethanol and Tris(hydroxymethyl) aminomethane (TRIS) (≥99.9%) were provided by Carl Roth (Karlsruhe, Germany), and formic acid (99% ULC/MS) by Biosolve (Valkenswaard, The Netherlands). Cholesterol esterase from Pseudomonas sp. was purchased from MP Biomedicals (Eschwege, Germany). All-E-astaxanthin standard in its free form (SML0982, ≥97%, 3S,3′S, from Blakslea trispora) was obtained from Sigma-Aldrich (Taufkirchen, Germany), and astaxanthin monopalmitate (1017, 3RS, 3′RS) was provided by CaroteNature (Münsingen, Switzerland).
9
Quantitative Analysis of Glycyrrhizic Acid
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Glycyrrhizic acid (GZA) from RxBiosciences (Bonanza way, Gaithersburg, MD20879, USA), analytical as well as LCMS grade ethanol (EtOH), and analytical grade acetone (ACE) were obtained from Merck (Darmstadt, Germany). For sample preparation, extraction, and UHPLC/MSMS mobile phase an in-house Pure Lab (ELGA, High Wycombe, UK) purified water (H2O) was used.
10
Quantification of Insect Repellents
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DEET (97%) was purchased from Sigma Aldrich (Buenos Aires, Argentina), IR3535 (99.6%) was a gift from Merck Argentina (Buenos Aires, Argentina), and analytical grade acetone was acquired from Merck Germany (Darmstadt, Germany).
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