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Methyl thiazolyl tetrazolium (mtt)

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MTT is a colorimetric assay used to measure the metabolic activity of cells. It is a commonly used technique in cell biology and biochemistry research.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Sangon (17 mentions) Microplate reader, by Thermo Fisher Scientific (8 mentions) Dimethyl sulfoxide (dmso), by Solarbio (3 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions) Microplate reader, by Bio-Rad (2 mentions) Methyl thiazolyl tetrazolium (mtt), by Genview (2 mentions) Spectramax i3, by Molecular Devices (2 mentions) Microplate reader, by Agilent Technologies (2 mentions) Microplate reader, by Molecular Devices (2 mentions) Multiskan ascent microplate reader, by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Multiskan mk microplate reader, by Thermo Fisher Scientific (1 mentions) Elisa reader, by Agilent Technologies (1 mentions) Crystal violet, by BBI Lifesciences (1 mentions) Epoch 2, by Agilent Technologies (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Quinidine, by Maclin Biochemical Technology (1 mentions) Cyclosporin a, by Maclin Biochemical Technology (1 mentions) Vb12 standard, by Merck Group (1 mentions) Penicillin streptomycin solution 100x, by Solarbio (1 mentions)

64 protocols using methyl thiazolyl tetrazolium (mtt)

1

Evaluating EGCG Effects on Myeloma Cells

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The RPMI8226 and U266 cells were grown with 1 × 10 4 cells per well in 96-well plates and incubated with increasing concentrations of EGCG as 10 μM, 20 μM and 40 μM for 24 h, 48 h and 72 h, respectively. Then, 20 μL of 5 mg/ mL 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT; Sangon Biotech, Shanghai, China) was added for another 4 h. The MTT formazan crystals were then dissolved with Dulbecco's modified Eagle's medium (DMEM) and their optical density (OD) was examined at 570 nm. All the experiments were performed in triplicate independently.
To detect the effect of EGCG combined with bortezomib on cell growth, U266 and RPMI8226 cells were incubated with EGCG (10 μM, 20 μM and 40 μM) alone and in combination with bortezomib (10 nM, 20 nM, 50 nM, 100 nM, and 200 nM) for 24 h. The MTT assay was used to determine the cell viability as described above.
Qiu X., Wu X, & He W. (2022). (-)-Epigallocatechin-3-gallate plays an antagonistic role in the antitumor effect of bortezomib in myeloma cells via activating Wnt/β-catenin signaling pathway. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 31(7).
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2

Cell Viability Assay with MTT

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One thousand cells were plated into 96-wells plates and incubated at 37 °C for 4 days and the medium was changed every other day. MTT (0.5 mg/ml) (Sangon Biotech, Shanghai, China) were added to the well at 8 hours (h), 24 h, 48 h, 72 h and 96 h after cells were plated. DMSO (Sangon Biotech, Shanghai, China) was applied to dissolve formazon crystals after the MTT incubation. At last, OD values at 490 nm were measured for every well. All samples were evaluated in six replicates in three independent experiments.
Qiu Q.C., Wang L., Jin S.S., Liu G.F., Liu J., Ma L., Mao R.F., Ma Y.Y., Zhao N., Chen M, & Lin B.Y. (2018). CHI3L1 promotes tumor progression by activating TGF-β signaling pathway in hepatocellular carcinoma. Scientific Reports, 8, 15029.
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3

MTT Assay for Cell Viability

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Cell viability was measured using an MTT assay. ACHN cells in logarithmic phase were cultured in 96-well plates (4×103 cells per well). Cells were exposed to sorafenib and celecoxib (Selleck Chemicals) at the indicated concentration for 48 h. After discarding the cell culture media, cells were washed and then incubated in 0.5 mg/ml MTT (Sangon Biotech Co., Ltd.) for 2 h at 37°C. MTT crystals were dissolved in DMSO (Sangon Biotech Co., Ltd.). The absorbance per well was proportional to the cell viability. The absorption value was measured at a wavelength of 490 nm using the infinite M200Pro system (Tecan Group, Ltd.).
Dai H., Wang G., Cao W., Qi W., Chen W, & Guo H. (2023). Stress granules affect the sensitivity of renal cancer cells to sorafenib by sequestering and stabilizing COX‑2 mRNA. Oncology Letters, 25(6), 274.
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4

MTT Cell Viability Assay Protocol

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Cells were plated into 96-well plates at a density of 5,000 cells/well and were treat with or without miR-133b inhibitor 12 h later. After a another incubation for 24, 48, and 72 h, 10 μl MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution (Sangon Biotech, China) was added to each well, the cells were cultured for 4 h at 37°C, and the medium was removed. Next, the cells were incubated with 150 μl dimethyl sulfoxide solution (MP Biomedicals, USA) for 10 min for cell lysis. The absorbance was measured at 570 nm using the Multiskan MK microplate reader (ThermoFisher Scientific, USA). All experiments were repeated three times.
Lv L., He L., Chen S., Yu Y., Che G., Tao X., Wang S., Jian Z, & Zhang X. (2019). Long Non-coding RNA LINC00114 Facilitates Colorectal Cancer Development Through EZH2/DNMT1-Induced miR-133b Suppression. Frontiers in Oncology, 9, 1383.
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5

Cell Proliferation Assay with NL101

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Cell proliferation was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)2 2,5-diphenyl tetrazolium bromide] assay kit (Sangon Biotech, China) according to the manufacturer's instructions. Cells were briefly seeded in 96-well plates and treated with gradient concentration of NL101 for 48 h. MTT was added at a final concentration of 0.5 mg/mL, and the cells were incubated for another 4 h. The supernatant was removed, DMSO was added, and the absorbance at 570 nm was read by a microplate reader.
Li S., He X., Gan Y., Zhang J., Gao F., Lin L., Qiu X., Yu T., Zhang X., Chen P., Tong J., Qian W, & Xu Y. (2021). Targeting miR-21 with NL101 blocks c-Myc/Mxd1 loop and inhibits the growth of B cell lymphoma. Theranostics, 11(7), 3439-3451.
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6

Quantifying Dendritic Cell Apoptosis

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Two days after LPS (1 µg/mL) stimulation (day 8), the cells were cultured in the starvation media (RPMI-1640 supplemented with 20-ng/mL rmGM-CSF, 10-ng/mL rmIL-4, and no serum) for 24 h to induce the apoptosis. To quantify cell viability, 100 mL of DCs were seeded (2.5 × 105 cells/mL) with starvation media in 96-well plates, and the MTT (Sangon Biotech shanghai, China) assay was performed according to manufacturer's instructions. Data are presented as cell activity relative to control samples. Alternatively, the cells were assessed by flow cytometry stained with fluorescein-isothiocytanate (FITC)-conjugated annexin V and PI, according to the manufacturer's instructions.
Dong Z., Chen Y., Peng Y., Wang F., Yang Z., Huang G., Chen Y., Yuan Z., Cao T, & Peng Y. (2017). Concurrent CCR7 Overexpression and RelB Knockdown in Immature Dendritic Cells Induces Immune Tolerance and Improves Skin-Graft Survival in a Murine Model. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 42(2).
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7

Assessing ELV Effects on Min6 Cells

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Min6 cells were incubated with human ELVs for 48 h. Then, 5 mg/ml MTT (Sangon Biotech, Shanghai, China) was added and incubated for another 4h. Optical density (OD) values were read at 490 nm.
Ge Q., Xie X., Chen X., Huang R., Rui C.X., Zhen Q., Hu R., Wu M., Xiao X, & Li X. (2021). Circulating exosome-like vesicles of humans with nondiabetic obesity impaired islet β-cell proliferation, which was associated with decreased Omentin-1 protein cargo. Genes & Diseases, 9(4), 1099-1113.
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8

Hypoxia Impacts on Cell Viability

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Cell growth and viability were assessed by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay according to a standard method. Briefly, cells were seeded in 96-well plates and cultured until 80% confluence was reached. After the cells had been administered recombinant adenovirus vectors [1 × 106 plaque-forming units (pfu) of virus per well], they were then cultured under normoxic conditions or subjected to hypoxia for 6, 12, 24 or 48 h. Thereafter, the culture medium was replaced by fresh medium and 20 μl PBS containing 5 mg/ml MTT (Sangon, China) were added per well. The cells were incubated for an additional 4 h. A total of 150 μl dimethyl sulfoxide were added per well for 15 min to dissolve the formazan crystals. Finally, the absorbance was measured at 490 nm using an ELISA reader (Bio-tek Instruments, USA). The experiment was performed in quadruplicate and was repeated thrice.
Sun L., Hao Y., An R., Li H., Xi C, & Shen G. (2014). Overexpression of Rcan1-1L Inhibits Hypoxia-Induced Cell Apoptosis through Induction of Mitophagy. Molecules and Cells, 37(11), 785-794.
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9

MTT Assay for Cell Proliferation

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3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay was used to measure cell proliferation. The cells were planted in 96-well-plates at densities of 1,000 cells per well in quintuplicate. Then, the cells were incubated with doxorubicin at a concentration of 8 nM. At assigned monitoring times, we added 20 μL 0.5 mg/mL MTT (Sigma-Aldrich, USA) to each well and incubated the cells with MTT for 4 h. Later, the supernatants were carefully discarded and 100 μL of dimethyl sulfoxide (Sangon Biotech, China) was added to each well. The absorbance value at 490 nm was evaluated by a microplate reader (Thermo Scientific, USA).
Zhang Z., Sun C., Li C., Jiao X., Griffin B.B., Dongol S., Wu H., Zhang C., Cao W., Dong R., Yang X., Zhang Q, & Kong B. (2020). Upregulated MELK Leads to Doxorubicin Chemoresistance and M2 Macrophage Polarization via the miR-34a/JAK2/STAT3 Pathway in Uterine Leiomyosarcoma. Frontiers in Oncology, 10, 453.
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10

MTT Assay for P. gingivalis Cytotoxicity

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After co-culturing 500 μL P. gingivalis suspensions (107 CFU/mL) with 500 μL TC at 1/4 × MIC, 1/2 × MIC, and 1 × MIC for 48 h individually, the culture supernatants were removed, and 250 μL MTT (0.5 mg/mL, Sangon Biotech, China) was added to each well for 4-h incubation at 37 °C in the dark. Subsequently, each well was washed with PBS thrice, and 250 μL dimethyl sulfoxide (DMSO, Tong Cheng Chemical Agent Co., Ltd., China) was added to each well to dissolve the formazan crystals, and was shaken for 15 min. Finally, the DMSO supernatants were transferred to a 96-well plate, and the OD values were recorded at a wavelength of 490 nm. The experiment was conducted in triplicate for each group.
Gan N., Fang Y., Weng W., Jiao T, & Yu W. (2023). Antibacterial effects and microarray-based molecular mechanisms of trans-cinnamaldehyde against Porphyromonas gingivalis. Heliyon, 9(12), e23048.
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