HEK293T cells (ATCC) were cultured in Dulbecco's modified Eagle's medium (Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS) and 1 × penicillin/streptomycin (P/S) (Nacalai Tesque). MT4 cells (ATCC) were cultured in RPMI supplemented with 10% FBS and 1 × P/S.
Mt4 cells
Manufactured by American Type Culture Collection
MT4 cells are a type of human T-cell line derived from the peripheral blood of a patient with adult T-cell leukemia/lymphoma. They are a widely used model for studying various aspects of T-cell biology and function.
Automatically generated - may contain errors
2 protocols using mt4 cells
1
Cell Culture Protocols for HEK293T and MT4 Cells
2
SARS-CoV-2 and HIV-1 Pseudovirus Infection Assay
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293T-hsACE2 cells (Integral Molecular, Philadelphia, PA), target cells for SARS-CoV-2 pv, were cultured in DMEM, 10% FBS, 50 µg/ml gentamicin, 2.5 μg/ml amphotericin B and 0.5 μg/ml puromycin. SARS-CoV-2 S protein (Wuhan-Hu-1:D614) pseudotyped lentiviral stocks (Virongy, Manassas, VA) contained green fluorescent protein (GFP) reporters and were quantified by p24 content.
MT-4 cells (ATCC, Manassas, VA), target cells for HIV-1LAI.04, were cultured in RPMI 1640, 10% FBS, 50 μg/ml gentamicin and 2.5 µg/ml amphotericin B. HIVLAI.04 viral stock (Virology Quality Assurance Laboratory, Rush University, Chicago, IL) was quantified by p24 content.
Cell experiments were performed with 0.25% ENC, and recombinant ACE2 receptor (ACE2) and anti-SARS-CoV-2 S IgG (IgG) from Quantitative SARS-Cov-2 Neutralizing Antibody ELISA kit diluted 1:10 in 0.9 M NaCl.
MT-4 cells (ATCC, Manassas, VA), target cells for HIV-1LAI.04, were cultured in RPMI 1640, 10% FBS, 50 μg/ml gentamicin and 2.5 µg/ml amphotericin B. HIVLAI.04 viral stock (Virology Quality Assurance Laboratory, Rush University, Chicago, IL) was quantified by p24 content.
Cell experiments were performed with 0.25% ENC, and recombinant ACE2 receptor (ACE2) and anti-SARS-CoV-2 S IgG (IgG) from Quantitative SARS-Cov-2 Neutralizing Antibody ELISA kit diluted 1:10 in 0.9 M NaCl.
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