Antibodies against phospho erk

Anti-GCLC and anti-NQO1 antibodies, anti-Nrf2 antibody, and anti-α-tubulin antibody were provided by Abcam (Cambridge, MA, USA), Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Rockland Immunochemicals (Gilbertsville, PA, USA), respectively. Antibodies against phospho-ERK, ERK, and lamin A/C were purchased from Cell Signaling Technology (Beverly, MA, USA). 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H₂DCFDA), PD98059, and Compound C were obtained from Invitrogen (Carlsbad, CA, USA), Millipore (Billerica, MA, USA), and Calbiochem (SanDiego, CA, USA), respectively. Anti-β-actin antibody, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), tert-butylhydroperoxide (t-BHP), LY294002, and other reagents were supplied by Sigma-Aldrich Co. (St. Louis, MO, USA).

Immunoblotting was performed using SDS-PAGE as previously described [29 ]. Antibodies against phospho-ERK, phospho-JNK, phospho-p38, phospho-IκBα, ERK, JNK, p38, and actin were purchased from Cell Signaling Technology (Boston, MA, USA). Antibody against capase-1 was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody against IL-1β was purchased from R&D Systems (Minneapolis, MN, USA)