Log In Sign up
The largest database of trusted experimental protocols

S6k1t389

Manufactured by Cell Signaling Technology
Visit Supplier

S6K1T389 is a primary antibody that detects phosphorylation of the Thr389 residue of the S6K1 (p70 S6 Kinase) protein. It is used to monitor the activation state of the mTOR signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

Akts473, by Cell Signaling Technology (7 mentions) Mtors2448, by Cell Signaling Technology (4 mentions) Bafilomycin, by Merck Group (4 mentions) Ulk1s757, by Cell Signaling Technology (4 mentions) Pf 4708671, by Merck Group (3 mentions) Sp600125, by Cell Signaling Technology (3 mentions) Anti actin mab, by Santa Cruz Biotechnology (3 mentions) Ulk1s555, by Cell Signaling Technology (3 mentions) Ampkt172, by Cell Signaling Technology (3 mentions) Tak1t184 187, by Cell Signaling Technology (3 mentions) Irs 1s1101, by Cell Signaling Technology (2 mentions) Iry1146, by Cell Signaling Technology (2 mentions) Dexamethasone (dex), by Merck Group (2 mentions) Rapamycin, by Cell Signaling Technology (2 mentions) Aktt308, by Cell Signaling Technology (2 mentions) Ly294002, by Cell Signaling Technology (2 mentions) 4e bpt37 46, by Cell Signaling Technology (2 mentions) U0126, by Cell Signaling Technology (2 mentions) Oligomycin, by Merck Group (2 mentions) 5z 7 oxozeaenol, by Merck Group (2 mentions)

7 protocols using s6k1t389

1

Gingerenone A Modulates Insulin Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gingerenone A was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). PF-4708671 and dexamethasone were obtained from Sigma Aldrich (St. Louis, MO). Rosiglitazone was obtained from Calbiochem (EMD Millipore, Billerica, MA). 3-Isobutyl-1-methylxanthine (IBMX). Insulin and 2-NBDG were purchased from Invitrogen (Life Technologies, Grand Island, NY). Antibodies against AKT, S6K1, S6, IRS-1, p85 of the PI-3 kinase and phospho-antibodies (AKTS473, pERKT202/204, S6K1T389, S6S235/236, IRS-1S1101, and IRY1146) were purchased from Cell Signaling Technology (Danvers, MA). A GLUT4 expression vector (mCherry-Glut4-myc) was obtained from Dr. Amira Klip (The Hospital for Sick Children, Toronto, Ontario).
Chen J., Sun J., Prinz R.A., Li Y, & Xu X. (2018). Gingerenone A sensitizes the insulin receptor and increases glucose uptake by inhibiting the activity of p70 S6 kinase. Molecular nutrition & food research, 62(23), e1800709.
+ Open protocol
+ Expand
2

Insulin Signaling Pathway Investigation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leflunomide and A77 1726 were kindly provided by CinKate Corporation (Oak Park, IL). Cytochalasin and rosiglitazone were purchased from Calbiochem (EMD Millipore, Billerica, MA). 3-Isobutyl-1-methylxanthine (IBMX), carboxymethyl-cellulose sodium (CMC), uridine, dexamethasone, and 2-deoxy-glucose (2-DG) were purchased from Sigma Aldrich (St. Louis, MO). 2-DG (5–10 Ci(185–370GBq)/mmol, 1mCi (37MBq) was purchased from PerkinElmer (Waltham, MA). Insulin used in the in vitro study was purchased from Invitrogen (Life Technologies, Grand Island, NY). Rapamycin (an mTOR inhibitor), antibodies against AKT, S6K1, S6, IRS-1, p85 of the PI-3 kinase and phospho-antibodies (AKTS473, AKTT308, S6K1T389, S6S235/236, IRS-1S1101, IRY1146, and IRS-1S636) were purchased from Cell Signaling Technology (Danvers, MA). Anti-β-actin antibody monoclonal antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The sources of antibodies and their applications were listed in Table 1. (mCherry-GLUT4-myc expression vector was kindly provided by Dr. Amira Klip (The Hospital for Sick Children, Toronto, Ontario). Use of the radioactive isotope was approved by Rush University Medical Center. All methods were performed in accordance with the relevant guidelines and regulations of Rush University Medical Center and Yangzhou University.
Chen J., Sun J., Doscas M.E., Ye J., Williamson A.J., Li Y., Li Y., Prinz R.A, & Xu X. (2018). Control of hyperglycemia in male mice by leflunomide: Mechanisms of action. The Journal of endocrinology, 237(1), 43-58.
+ Open protocol
+ Expand
3

Signaling Pathway Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leflunomide and A77 1726 were kindly provided by Cinkate Corporation (Oak Park, IL). PPP was purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). U0126, LY294002, and rapamycin were purchased from Cell Signaling Technology (Danvers, MA). OSI-906 was purchased from Selleckchem.com (Houston, TX). Antibodies against ERK1/2, MEK1/2, Raf-1, p90 RSK, GSKα/β, 4E-BP, PDK1, AKT, mTOR, S6K1, and S6 and their corresponding phosphor antibodies including ERK1/2T202/Y204, MEK1/2S217/S221, Raf-1S338, p90 RSKT356/S360, GSKα/βS21/9, 4E-BPT37/46, PDKS241, AKTS473, AKTT308, mTORS2448, S6K1T389, S6S235/236 insulin receptor substrate-1S1101 (IRS-1S1101), and CADS1859 were purchased from Cell Signaling Technology.
Doscas M.E., Williamson A.J., Usha L., Bogachkov Y., Rao G.S., Xiao F., Wang Y., Ruby C., Kaufman H., Zhou J., Williams J.W., Li Y, & Xu X. (2014). Inhibition of p70 S6 Kinase (S6K1) Activity by A77 1726 and Its Effect on Cell Proliferation and Cell Cycle Progress. Neoplasia (New York, N.Y.), 16(10), 824-834.
+ Open protocol
+ Expand
4

Autophagy Regulation in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leflunomide and A77 1726 were kindly provided by Cinkate Corporation (Oak Park, IL). Brequinar sodium (BQR), a potent inhibitor of DHO-DHase, was kindly provided by Dupont Corporate (Wilmington, DE). PLX4720 was purchased from Selleck Chemicals Inc. (Houston, TX). SP600125, U0126, PD98059, and LY294002 were purchased from Cell Signaling Technology (Danvers, MA). Rapamycin was purchased from Cayman Laboratories (Ann Arbor, MI). Bafilomycin, colchicine metformin, 5Z-7-oxozeaenol, PF-4708671, and oligomycin were purchased from Sigma (St. Louis, MO). Anti-actin mAb and PPP were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against p62, LC3, ULK1, AMPK, JNK, Jun, AKT, S6K1, S6 and their corresponding phospho-antibodies including ULK1S555, ULK1S757, AMPKT172, AKTS473, S6K1T389, S6S235/236, TAK1T184/187 were purchased from Cell Signaling Technology (Danvers, MA). The expression vector encoding RFP-LC3 (pmRFP-LC3) was purchased from OriGene Technologies, Inc. (Rockville, MD).
Xu X., Sun §., Song R., Doscas M.E., Williamson A.J., Zhou J., Sun J., Jiao X., Liu X, & Li Y. (2017). Inhibition of p70 S6 kinase (S6K1) activity by A77 1726, the active metabolite of leflunomide, induces autophagy through TAK1-mediated AMPK and JNK activation. Oncotarget, 8(18), 30438-30454.
+ Open protocol
+ Expand
5

Autophagy Regulation in Influenza Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
SP600125 was purchased from Cell Signaling Technology (Danvers, MA). Rapamycin was purchased from Cayman Laboratories (Ann Arbor, MI). Bafilomycin and chloroquine (CQ) were purchased from Sigma (St. Louis, MO). Anti-β-actin mAb was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against p62, Beclin-1, LC3, AKT, S6K1, and their corresponding phospho-antibodies including Beclin-1S93, ULK1S757, mTORS2448, AKTS473, and S6K1T389 were purchased from Cell Signaling Technology (Danvers, MA). The expression vector encoding RFP-LC3 (pmRFP-LC3) was purchased from OriGene Technologies, Inc. (Rockville, MD). DF-1 cells were purchased from the American Tissue Culture Collection (Manassas, VA) and grown in complete DMEM medium supplemented with 10% fetal bovine serum, streptomycin and penicillin, and l-glutamine. Monoclonal or polyclonal antibodies against NP, M1, and HA proteins were prepared by immunizing mice with purified recombinant proteins.
Zhang J., Ruan T., Sheng T., Wang J., Sun J., Wang J., Prinz R.A., Peng D., Liu X, & Xu X. (2018). Role of c-Jun terminal kinase (JNK) activation in influenza A virus-induced autophagy and replication. Virology, 526, 1-12.
+ Open protocol
+ Expand
6

Investigating Autophagy Regulation in ALS Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leflunomide and A77 1726 were kindly provided by Cinkate Corporation (Oak Park, IL). SP600125 was purchased from Cell Signaling Technology (Danvers, MA). Rapamycin was purchased from Cayman Laboratories (Ann Arbor, MI). Bafilomycin, colchicine, metformin, 5Z-7-oxozeaenol, PF-4708671, and oligomycin were purchased from Sigma (St. Louis, MO). Anti-actin mAb was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against LC3, ULK1, AMPK, AKT, S6K1, S6, ACC (acetyl-CoA carboxylase) and their corresponding phospho-antibodies including ULK1S555, ULK1S757, AMPKT172, mTORS2448, AKTS473, S6K1T389, S6S235/236, ACCS79, and TAK1T184/187 were purchased from Cell Signaling Technology (Danvers, MA). Anti-SOD1 antibody was kindly provided by Dr. Han-Xiang Deng (Northwestern University, Chicago). The SOD1-GFP and SOD1G93A-GFP expression vectors were prepared by inserting a GFP gene downstream of SOD1 in a pcDNA3.1 vector. The expression vector encoding RFP-LC3 (pmRFP-LC3) was purchased from OriGene Technologies, Inc. (Rockville, MD). The NSC34 cell line was complete DMEM medium supplemented with 10% fetal bovine serum, streptomycin and penicillin, and L-glutamine.
Sun J., Mu Y., Jiang Y., Song R., Yi J., Zhou J., Sun J., Jiao X., Prinz R.A., Li Y, & Xu X. (2018). Inhibition of p70 S6 kinase activity by A77 1726 induces autophagy and enhances the degradation of superoxide dismutase 1 (SOD1) protein aggregates. Cell Death & Disease, 9(3), 407.
+ Open protocol
+ Expand
7

Bafilomycin, Chloroquine, and 5Z-7-Oxozeaenol Autophagy Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bafilomycin, CQ, and 5Z were purchased from Sigma (St. Louis, MO). CC was purchased from Selleck Inc. (Houston, TX). Anti-actin mAb was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Antibodies against LC3, ULK1, AMPK, mTOR, AKT, S6K1, 4E-BP, S6, ACC (acetyl-CoA carboxylase), TAK1, and their corresponding phospho-antibodies including ULK1S555, ULK1S757, ULK1S317, AMPKT172, mTORS2448, AKTS473, S6K1T389, S6S235/236, 4E-BPT37/46, IKKαS176/180, ACCS79, and TAK1T184/187 were purchased from Cell Signaling Technology (Danvers, MA).
Liu W., Jiang Y., Sun J., Geng S., Pan Z., Prinz R.A., Wang C., Sun J., Jiao X, & Xu X. (2018). Activation of TGF-β-activated kinase 1 (TAK1) restricts Salmonella Typhimurium growth by inducing AMPK activation and autophagy. Cell Death & Disease, 9(5), 570.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!