All chemicals were of analytical grade, sourced from commercial suppliers (Sigma-Aldrich, St. Louis, MO, USA, Fisher Scientific, Hampton, NH, USA) and were used without further purification unless otherwise specified. Phosphate-buffered saline (PBS) at pH 7.4 was prepared by dissolving 1.44 g Na2HPO4, 0.2 g KCl, 0.24 g KH2PO4, and 8 g NaCl in purified water (1 L) and autoclaved at 121 °C for 20 min. Absorbance spectra were measured on a Hitachi U-3010 spectrophotometer (Hitachi High-Technologies Corporation, Tokyo, Japan) while fluorescence data were recorded on Hitachi F-2500 fluorescence spectrophotometer. Cell imaging was carried out using a Zeiss LSM 700 confocal microscope (Carl Zeiss, Oberkochen, Germany). Escherechia coli (BL21) cells were used throughout this study and were purchased from Transgen Biotech, Beijing, China. Enumeration of bacteria was expressed in CFU mL−1.
U 3010 spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The U-3010 spectrophotometer is a laboratory instrument manufactured by Hitachi that measures the absorbance or transmittance of light through a sample across a range of wavelengths. It provides accurate and reliable data for various applications that require spectroscopic analysis.
Automatically generated - may contain errors
Lab products found in correlation
Ft ir 4200 spectrometer, by Jasco (9 mentions)
P 1020 polarimeter, by Jasco (8 mentions)
Jms 700 mass spectrometer, by JEOL (6 mentions)
Ri 150, by Gilson (4 mentions)
Uv vis 151, by Gilson (4 mentions)
6130 quadrupole mass spectrometer, by Agilent Technologies (3 mentions)
6224 tof lc ms spectrometer, by Agilent Technologies (3 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (3 mentions)
1200 series hplc, by Agilent Technologies (2 mentions)
Avance 600 mhz spectrometer, by Bruker (2 mentions)
Chirascan plus cd spectrometer, by Applied Photophysics (2 mentions)
5600 qtof hr ms instrument, by AB Sciex (2 mentions)
Avance 500 mhz instrument, by Bruker (2 mentions)
Zetasizer ver 7, by Malvern Panalytical (2 mentions)
Su8020, by Hitachi (2 mentions)
Mal1032660, by Malvern Panalytical (2 mentions)
Biflex 3 spectrometer, by Bruker (2 mentions)
F 4500 fluorescence spectrophotometer, by Hitachi (2 mentions)
6520 quadrupole time of flight mass spectrometer, by Agilent Technologies (2 mentions)
Ir 200 spectrophotometer, by Thermo Fisher Scientific (2 mentions)
70 protocols using u 3010 spectrophotometer
1
Bacterial cell imaging and enumeration
2
Comprehensive Analytical Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotation was measured on a JASCO P-1020 polarimeter (Jasco, Tokyo, Japan) using a 1 cm cell. The UV spectra were recorded on a Hitachi U-3010 spectrophotometer (Hitachi High-Technologies, Tokyo, Japan), and the IR spectra were recorded on a JASCO 4200 FT-IR spectrometer (Jasco, Tokyo, Japan) using a ZnSe cell. NMR spectra were recorded in CDCl3 containing Me4Si as an internal standard on Bruker Avance 600 spectrometers (Bruker, Karlsruhe, Germany). Proton and carbon NMR spectra were measured at 600 and 150 MHz, respectively. High-resolution ESI-Q-ToF-MS/MS mass spectrometric data were obtained on an Agilent Technologies 6530 Accurate-Mass Q-ToF LC/MS spectrometer (Santa Clara, CA, USA) with an Agilent Technologies 1260 series HPLC. Low-resolution ESIMS data were recorded on an Agilent Technologies 6130 quadrupole mass spectrometer with an Agilent Technologies 1200 series HPLC. HPLC was performed on a Shimadzu LC-6AD equipped with a Shimadzu RID-10A refractive index detector (Shimadzu, Kyoto, Japan). All solvents were spectroscopic grade or distilled in a glass prior to use.
3
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured using a JASCO P-1020 polarimeter (Jasco, Tokyo, Japan) with a 1-cm cell. CD spectra were obtained using an Applied Photophysics Ltd. Chirascan Plus spectrometer (Leatherhead, Surrey, UK). UV spectra were acquired using a Hitachi U-3010 spectrophotometer (Hitachi High-Technologies, Tokyo, Japan). IR spectra were recorded on a JASCO 4200 FT-IR spectrometer (Jasco, Tokyo, Japan) using a ZnSe cell. NMR spectra were recorded in MeOH-d3, MeOH-d4 and CDCl3 with the solvent peaks (δH 3.30/δc 47.5 and δH 7.26/δc 77.0) as internal standards on a Bruker Avance 600 MHz spectrometer (Billerica, MA, USA). Proton and carbon NMR spectra were measured at 600 and 150 MHz, respectively. High-resolution FABMS data were obtained at the National Center for Inter-university Research Facilities (NCIRF), Seoul National University, and acquired using a JEOL JMS 700 mass spectrometer (Jeol, Tokyo, Japan) with 6 keV-energy, emission current 5.0 mA, xenon as the inert gas, and meta-nitrobenzyl alcohol (NBA) as the matrix. HPLC separations were performed on a SpectraSYSTEM p2000 equipped with a refractive index detector (SpectraSYSTEM RI-150 (Waltham, MA, USA)) and a UV-Vis detector (Gilson UV-Vis-151 (Middleton, WI, USA)). All solvents used were of spectroscopic grade or were distilled prior to use.
4
Spectroscopic Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on a JASCO P1020 polarimeter (Jasco, Tokyo, Japan) using a 1 cm cell. Ultraviolet (UV) spectra were acquired with a Hitachi U-3010 spectrophotometer (Hitachi High-Technologies, Tokyo, Japan). ECD spectra were recorded on a Chirascan plus CD spectrometer (Applied Photophysics Ltd., Leatherhead, Surrey, UK). IR spectra were recorded on a JASCO 4200 FT-IR spectrometer (Jasco, Tokyo, Japan) using a ZnSe cell. 1H and 13C NMR spectra were measured in DMSO-d6 or MeOH-d4 solutions on a Bruker Avance −400, −500, −600, or −800 instrument (Billerica, MA, USA), with solvent peaks at δH 2.50/δC 39.50 and δH 3.31/δC 49.00 as their internal standards. High-resolution ESI mass spectrometric data were obtained at the National Instrumentation Center for Environmental Management (Seoul, Korea) and were acquired using an AB Sciex 5600 QTOF HR-MS instrument (Sciex, MA, USA). High-performance liquid chromatography (HPLC) analysis was conducted using a Shimadzu SCL-10A (Shimadzu, Tokyo, Japan) control system connected to a UV–Vis SPD-10A detector (Shimadzu). All solvents used were of spectroscopic grade or distilled from glass prior to use.
5
Comprehensive Analytical Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on a JASCO P1020 polarimeter (Jasco, Tokyo, Japan) using a 1 cm cell. UV spectra were acquired with a Hitachi U-3010 spectrophotometer (Hitachi High-Technologies, Tokyo, Japan). IR spectra were recorded on a JASCO 4200 FT-IR spectrometer (Jasco, Tokyo, Japan) using a ZnSe cell. 1H and 13C NMR spectra were measured in DMSO-d6, CDCl3, or CD3OD solutions on Bruker Avance –400, –500, –600, or –800 instruments (Billerica, MA, USA). High resolution FAB mass spectrometric data were obtained at the Korea Basic Science Institute (Daegu, Korea) and were acquired using a JEOL JMS 700 mass spectrometer (Jeol, Tokyo, Japan) with meta-nitrobenzyl alcohol (NBA) as the matrix. High-resolution LC-MS/MS data were obtained at the National Instrumentation Center for Environmental Management (Seoul, Korea) on a Q-TOF 5600 instrument equipped with a Dionex U-3000 HPLC system. Semi-preparative HPLC separations were performed on a Spectrasystem p2000 equipped with a Spectrasystem RI-150 refractive index detector. All solvents used were spectroscopic grade or distilled from glass prior to use.
6
Oxidative Stability Determination of Chicken Meat
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxidative stability determination was based on malondialdehyde (MDA) content. The MDA concentration was measured in the breast meat fillet sample (left pectoralis major muscle) from 10 chickens per treatment (i.e., 2 birds per replicate cage). Measurements were processed by using the selective third-order derivative spectrophotometric method after storage at 4 °C for 1, 3, 6 and 9 days and −20 °C for 30 and 60 days after slaughter in plastic sealed bags. In particular, 2 g of each meat sample (two samples per chicken) was homogenized in 8 mL aqueous trichloroacetic acid (TCA) (50 g/L) and 5 mL butylated hydroxytoluene in hexane (8 g/L), and then the solution was centrifuged for 5 min at 3000× g. Then, the top hexane layer was removed, and 1.5 mL of aqueous 2-thiobarbituric acid (8 g/L) was added to 2.5 mL from the bottom layer to be further incubated at 70° C for 30 min. This mixture was cooled under tap water and processed with third-order derivative (3D) spectrophotometry (Hitachi U3010 Spectrophotometer, Hitachi High-Technologies Corporation, Japan) in the range of 500–550 nm. The final concentration of MDA (ng/g wet tissue) was calculated as the height of the third-order derivative peak at 521.5 nm by referring to the standard calibration curve prepared using 1,1,3,3-tetraethoxypropane [26 (link)].
7
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured using a JASCO P-1020 polarimeter (Jasco, Tokyo, Japan) with a 1 cm cell. UV spectra were acquired using a Hitachi U-3010 spectrophotometer (Hitachi, Tokyo, Japan). IR spectra were recorded on a JASCO 4200 FT-IR spectrometer (JASCO, Easton, MD, USA) using a ZnSe cell. NMR spectra were recorded in CDCl3 with the solvent peaks (δH 7.26/δc 77.2) as internal standards on a JEOL JNM 400 MHz NMR spectrometer (JOEL, Peabody, MA, USA). HR-FAB-MS data were obtained at the National Center for Inter-University Research Facilities (NCIRF), Seoul National University, and acquired using a JEOL JMS 700 mass spectrometer (Jeol, Tokyo, Japan) with 6 keV-energy, emission current 5.0 mA, xenon as inert gas, and meta-nitrobenzyl alcohol as the matrix. HPLC separations were performed on a SpectraSYSTEM p2000 equipped with a refractive index detector (SpectraSYSTEM RI-150; Thermo Scientific, Waltham, MA, USA) and a UV-Vis detector (Gilson UV-Vis-151, Gilson, Middleton, WI, USA). All solvents used were of spectroscopic grade or were distilled prior to use.
8
Detailed Characterization of Bacterial Polyhydroxyalkanoate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chain structure of the PHA produced by the alginolytic bacteria was first investigated by the crotonic acid method (32) . Briefly, 0.01 mL of PHA (1 mg/mL in chloroform) was added to 10 mL of concentrated sulfuric acid and incubated at 100 °C for 10 min. After cooling at room temperature, UV absorption spectrum was recorded with U-3010 spectrophotometer (Hitachi High Tech Science, Tokyo, Japan). Formation of crotonic acid from PHB was assessed by detecting maximum absorption at 235 nm. Structure of PHA was also analyzed by 1 H-NMR with ECP-400 NMR spectrometer (400 MHz; JOEL, Tokyo, Japan) at the Global Facility Center of Hokkaido University, Japan. Differential scanning calorimetry (DSC) profile of PHA was determined with a DSC1 calorimeter (Mettler, Tokyo, Japan). Temperature scanning was carried out at 10 °C/min from -50 °C to 200 °C with a constant nitrogen flow at 50 mL/min. Viscosity of PHA in chloroform was determined with an Ostwald viscometer (flow time for solvent; 16 s). Briefly, PHA was dissolved in chloroform at 60 °C to make 1.0 -4.0 mg/mL and the flow time of the PHA solution was measured at 30 °C to determine the intrinsic viscosity [η]. Molecular weight of PHA was estimated using the formula of Mark-Houwink-Sakurada [η] = KM α with K and α values, 1.18 x 10 -5 mL/mg and 0.78, respectively (33) .
9
Absorption Spectrum of Photosynthetic Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Absorption spectra were recorded using a Hitachi U-3010 spectrophotometer. A blank measurement was recorded in Buffer A (+0.03% LDAO for detergent purified BRC). Samples were incubated in a PCR thermocycler at the indicated temperature, and then measured at the desired time points in a quartz cuvette at room temperature. Spectra were collected between 600 nm and 1100 nm (scan time ∼20 s) at intervals of 1.5 min. For comparisons of spectra between conditions, spectra were normalized to a value of 1.0 at 804 nm.
10
Multimodal Characterization of Nanomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1H NMR and 13C NMR spectra were measured with Bruker Avance 600 MHz NMR Spectrometer. High resolution mass spectrometer (HR-MS) was recorded on a Bruker MAXIS IMPACT mass spectrometer. MALDI-TOF was recorded on a MALDI SYNAPT G2-Si mass spectrometer. Near-infrared II (NIR-II) fluorescence spectra were recorded on NIRQUEST512 spectrometer (excitation: 808 nm laser, emission range: 900–1700 nm). The absorption spectra were collected on Hitachi U-3010 spectrophotometer. The NIR-II fluorescence (in vivo and ex vivo) imaging was performed by using NIR-II in Vivo Imaging System (Series II 808/900–1700, Suzhou NIR-Optics Technologies Co., Ltd.). The particle size and distribution were measured through DLS by using a Malvern Nano-ZS90 analyzer at a fixed angle of 90° at 25 °C. TEM experiments were performed with a JEM-1400 TEM transmission electron microscope (copper mesh with ultra-thin carbon film as the sample carrier). Optoacoustic imaging was performed on an inVision128 multispectral optoacoustic tomographic imaging system (iThera Medical GmbH).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!