Circulating progesterone levels were assessed in plasma samples obtained at the time of genital sampling using a solid-phase 125I radioimmunoassay kit, Coat-A-Count® Progesterone (Siemens Healthcare Diagnostics Inc., Tarrytown, NY). EDTA-treated plasma samples were processed according to the manufacturer’s basic protocol and counted for one minute on a 1480 Wizard Gamma Counter (Perkin Elmer, Waltham, MA). Final concentrations were determined by extrapolation of counts per minute to a calibration curve. The limit of detection in this assay is 0.02 ng/mL.
Coat a count progesterone
Manufactured by Siemens
Sourced in United States
The Coat-A-Count Progesterone is a laboratory equipment used for the quantitative determination of progesterone in human serum or plasma. It utilizes a solid-phase radioimmunoassay technique.
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Lab products found in correlation
6 protocols using coat a count progesterone
1
Plasma Progesterone Radioimmunoassay
2
Progesterone and Estradiol Level Assay
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The determination of the levels of E and P4 was developed monthly by solid phase RIA using the following packages: Coat-A-Count Progesterone and Coat-A-Count Estradiol (Siemens Healthcare Diagnostics, Inc., Los Angeles, CA.). The first with analytical sensitivity of 0.02 ng/ml, and an intra-assay coefficient of variation and inter 9.51 and 8.96%, respectively, and the second with analytical sensitivity of 8 pg/ml, with an intra-assay coefficient of variation and inter 5.92% and 6.0%, respectively. The test was developed in the Laboratory of Endocrinology, Department of Reproduction, Faculty of Veterinary Medicine and Animal Husbandry of the National Autonomous University of Mexico.
3
Serum Estradiol and Progesterone Assay
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Serum estradiol and progesterone levels were determined with the Coat-A-Count Estradiol and the Coat-A-Count Progesterone kits (Siemens Medical Solutions, Netherlands). These are no-extraction, solid-phase 125 I radioimmunoassay kits designed for measurement of estradiol and progesterone in serum.
4
Estrus Detection in Dairy Cows
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Visual observation of cows for four 30-min periods at 0330, 1000, 1430, and 2200 h occurred on d 2, 3, 4, and 5 (Figure 1). Two observers were present at each shift, with one assigned to each side of the separated housing area. Study cows were clearly identified using spray paint. Observers recorded the time of each standing estrus event.
Blood samples (10 mL) were collected from cow coccygeal veins on d -2, -1, 0, 1, 2, 7, 9, and 11 (Figure 1). Plasma was separated from centrifuged samples (2,800 rpm for 20 min at room temperature) and stored at -20°C until the concentration of P4 was determined by RIA (Coat-a-Count Progesterone, Siemens Medical Solutions USA Inc., Malvern, PA). Response to the synchronization protocol was confirmed if P4 was greater than 1.0 ng/mL on d -2, -1, and 0, dropped to less than 1.0 ng/mL by d 1, and returned above 1.0 ng/mL by d 9. We used P4 results to determine sensitivity, specificity, and accuracy of visual observation. Only validated standing estrus events were used to describe estrus-related changes in AED parameters and to explore estrus-detection potential of machinelearning techniques.
Blood samples (10 mL) were collected from cow coccygeal veins on d -2, -1, 0, 1, 2, 7, 9, and 11 (Figure 1). Plasma was separated from centrifuged samples (2,800 rpm for 20 min at room temperature) and stored at -20°C until the concentration of P4 was determined by RIA (Coat-a-Count Progesterone, Siemens Medical Solutions USA Inc., Malvern, PA). Response to the synchronization protocol was confirmed if P4 was greater than 1.0 ng/mL on d -2, -1, and 0, dropped to less than 1.0 ng/mL by d 1, and returned above 1.0 ng/mL by d 9. We used P4 results to determine sensitivity, specificity, and accuracy of visual observation. Only validated standing estrus events were used to describe estrus-related changes in AED parameters and to explore estrus-detection potential of machinelearning techniques.
5
Milk Progesterone Monitoring for Postpartum Interval
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Progesterone was measured in milk twice weekly (a.m. milking on each Tuesday and Friday) from 14 d in milk through to Sep 30. Progesterone concentrations were determined using a commercial radioimmunoassay kit (Progesterone Coat-A-Count; Siemens, Los Angeles, CA). The average intra-assay coefficients of variation were 8.7% and 20.9% for the high and low controls, respectively and the average inter-assay coefficients of variation were 10.5% and 3.5% for the high and low controls (n = 11 assays), respectively. The minimum detectable concentration of progesterone was 0.07 ng/mL. The PPAI was defined as the interval from calving to the first of two consecutive samples with progesterone concentration ≥ 1.95 ng/mL [19 (link)]. Milk progesterone analyses were halted as cows reached the threshold above.
6
Progesterone Assay Validation Protocol
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Medium P4 concentrations were determined by validated RIA (r2 = 0.986 and P < 0.0007), using the commercial kit Progesterone Coat-a-Count® (Siemens Medical Solutions Diagnostics, USA). The standard curve ranged from 0.16 to 55.2 ng ml-1. The sensibility was 92% and the intra- and interassay CVs were 3.14% and 2.88%, respectively.
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