Rat plasma was used to determine the levels of cytokines and chemokines, including tissue inhibitor of metallopeptidase-1 (TIMP-1), intercellular adhesion molecule-1 (ICAM-1), CXCL7, CD62L, VEGF, CX3CL1, LIX, CXCL1, CCL20, and CCL5, using an Rat Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s specifications.
Rat cytokine array kit
Manufactured by R&D Systems
Sourced in United States
The Rat Cytokine Array Kit is a multiplex assay designed to detect the relative levels of multiple rat cytokines and chemokines simultaneously in a single sample. The kit includes a nitrocellulose membrane pre-spotted with capture antibodies for the target analytes and detection reagents for signal development. This product enables researchers to obtain a broad profile of protein expression in rat biological samples.
Automatically generated - may contain errors
2 protocols using rat cytokine array kit
1
Rat Cytokine Profiling Protocol
2
Protein Profiling of Xenograft Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein lysates were prepared from snap frozen xenografts, and proteins (400 μg) were incubated with the Rat Cytokine Array Kit, Panel A, and the Human Angiogenesis Array Kit (R&D Systems; Minneapolis, MN, USA) according to the manufacturer's instructions. Collection and quantification of pixel densities from membranes was performed using the Fuji Las3000 (Fujifilm; Tokyo, Japan) and Image Reader LAS-3000 (Version 2.2; Fujifilm). Arrays for all samples were performed in duplicate, and an average density was calculated for each corresponding protein probed on the arrays based on the two pixel densities determined for each experiment.
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