Nah2po4

Purified soy phospholipid was a commercially available mixture (Lipoid S75, Lipoid, Ludwigshafen, Germany) containing ~70% phosphatidylcholine. Na₂HPO₄, NaH₂PO₄ and glycerol were from Carl Roth (Karlsruhe, Germany) and of analytical grade. Perfluorooctylbromide (purity 99%) and perfluorodecylbromide (purity 98%) were from ABCR (Karlsruhe, Germany).
The applied buffer contained 10 mM phosphate (7mM Na₂HPO₄, 3 mM NaH₂PO₄) and was isotonized with glycerol (2.5% w/w), the pH was adjusted to 7.2. For all preparation or analysis steps buffer was filtered and degased.

Aphanizomenon sp. ULC602, Anabaena sp. ULC080 and Microcystis sp. ULC 641 was obtained from a Belgian coordinated collection of microorganisms (BCCM, University of Liège, Cyanobacteria collection-ULC, Liège, Belgium) [5 (link)]. Aphanizomenon sp. ULC602 was grown in a 50% dilution of BG110 medium, which was prepared following the manufacturer’s guideline at laboratory room temperature (RT), 23 ± 1 °C, under continuous lighting. Anabaena sp. ULC080 was grown in BG110 medium (Table S1), which lacks a nitrate source, at 18 °C under continuous lighting. Microcystis sp. ULC641 was cultivated in standard BG11 medium prepared following the manufacturer’s guidelines (Table S2) at RT under continuous lighting. E. coli ATCC11330 from cultures collection of UWK (University for Continuing Education Krems) was grown in LB (Luria Broth) medium at 37 °C and 150 rpm. Chemicals for the preparation of culture media were purchased from CarlRoth (Vienna, Austria). Tris buffer, TCEP (tris-(2-carboxyethyl) phosphine hydrochloride), and 6-mercapto-1-hexanol (MCH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). NaH₂PO₄, Na₂HPO₄, NaCl, MgCl₂, and PBS (phosphate-buffered saline) were supplied by CarlRoth (Vienna, Austria).

PTX-2 standard was purchased from the National Research Council Institute for Marine Biosciences (Halifax, NS, Canada). Omeprazole, rifampicin, 3-methylcholanthrene, SR12813, CITCO, ethoxyresorufin, resorufin, and formate ammonium were purchased from Sigma-Aldrich (St. Louis, MO, USA). Reduced nicotinamide adeninedinucleotide phosphate (NADP+), glucose 6-phosphate (G6P), magnesium chloride hexahydrate, potassium chloride, Na₂HPO₄, and NaH₂PO₄ were purchased from Carl Roth (Karlsruhe, Germany). All of the other chemicals, including acetonitrile (ACN), methanol (MeOH), and dimethyl sulfoxide (DMSO) were of analytical grade and purchased from Fisher Scientific (Leicestershire, UK). Formic acid was purchased from Merck (Darmstadt, Germany). The deionised water was prepared using a Milli-Q system (Millipore, Bedford, MA, USA). The β-naphtoflavone and phenobarbital-induced Sprague Dawley rat and human hepatic S9 fractions were purchased from Biopredic International (Rennes, France).