Log In Sign up
The largest database of trusted experimental protocols

Enzymatic reagent set

Manufactured by Horiba
Visit Supplier
Sourced in United States

The Enzymatic Reagent Set is a lab equipment product designed to facilitate enzymatic analysis. It provides the necessary reagents and components to perform enzymatic assays in a research or clinical laboratory setting. The core function of this product is to enable the measurement and quantification of enzymatic activity in samples.

Automatically generated - may contain errors

Lab products found in correlation

Albuwell m, by Exocell (4 mentions) Quantichrom urea assay kit diur 500, by BioAssay Systems (3 mentions) Coomassie stain, by Bio-Rad (2 mentions) Creatinine companion, by Exocell (2 mentions) Quantichrom urea assay kit, by BioAssay Systems (1 mentions)

4 protocols using enzymatic reagent set

1

Urinary Biomarkers for Kidney Function Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Urinary albumin levels were determined by ELISA using a murine microalbuminuria ELISA kit, AlbuwellM™ (Exocell Inc., Philadelphia, PA, USA). The urine creatinine concentration was measured with a microplate assay kit, Creatinine Companion (Exocell Inc., Philadelphia, PA, USA). All measurements were performed in duplicate and albuminuria was determined as the ratio of urinary albumin (µg/ml) to creatinine (mg/ml). For electrophoresis, urine was centrifuged at 17,000 × g for 15 min at 4 °C to remove urinary sediment including whole cells, large membrane fragments, and other debris. 3 µl of the remaining suspension was added to an equal volume of 2 × Laemmli sample buffer and run on a 15% gel under non-reducing conditions. The gel was stained with Coomassie stain (BioRad). BUN was detected with QuantiChrom™ Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA) according to manufacturer’s instructions. Serum creatinine was detected with (Enzymatic) Reagent Set from Pointe Scientific, Inc. (Canton, MI).
Cheng H., Fan X., Lawson W.E., Paueksakon P, & Harris R.C. (2015). Telomerase deficiency delays renal recovery in mice after ischemia reperfusion injury by impairing autophagy. Kidney international, 88(1), 85-94.
+ Open protocol
+ Expand
2

Quantifying Proteinuria and Renal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteinuria levels were determined by enzyme-linked immunosorbent assay (ELISA) using a murine microalbuminuria ELISA kit (AlbuwellM™; Exocell Inc., Philadelphia, PA, USA). Urea nitrogen was detected with a QuantiChrom™ Urea Assay Kit (DIUR-500; BioAssay Systems, Hayward, CA, USA) according to manufacturer’s instructions. Serum creatinine was detected with a (Enzymatic) Reagent Set from Pointe Scientific, Inc. (MI, USA).
Wei H., Wang J, & Liang Z. (2020). STAT1-p53-p21axis-dependent stress-induced progression of chronic nephrosis in adriamycin-induced mouse model. Annals of Translational Medicine, 8(16), 1002.
+ Open protocol
+ Expand
3

Murine Microalbuminuria and Kidney Health

Check if the same lab product or an alternative is used in the 5 most similar protocols
A murine microalbuminuria ELISA kit, AlbuwellM™ (Exocell Inc., Philadelphia, PA, USA) was used to detect proteinuria levels. BUN was tested using the QuantiChrom™ Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA, USA) in line with the manufacturer’s instructions. Serum Cr was measured using the (Enzymatic) Reagent Set from Pointe Scientific, Inc. (Canton, MI, USA).
Tian Y., Shu J., Huang R., Chu X, & Mei X. (2020). Protective effect of renal ischemic postconditioning in renal ischemic-reperfusion injury. Translational Andrology and Urology, 9(3), 1356-1365.
+ Open protocol
+ Expand
4

Urinary Biomarkers for Kidney Function Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Urinary albumin levels were determined by ELISA using a murine microalbuminuria ELISA kit, AlbuwellM™ (Exocell Inc., Philadelphia, PA, USA). The urine creatinine concentration was measured with a microplate assay kit, Creatinine Companion (Exocell Inc., Philadelphia, PA, USA). All measurements were performed in duplicate and albuminuria was determined as the ratio of urinary albumin (µg/ml) to creatinine (mg/ml). For electrophoresis, urine was centrifuged at 17,000 × g for 15 min at 4 °C to remove urinary sediment including whole cells, large membrane fragments, and other debris. 3 µl of the remaining suspension was added to an equal volume of 2 × Laemmli sample buffer and run on a 15% gel under non-reducing conditions. The gel was stained with Coomassie stain (BioRad). BUN was detected with QuantiChrom™ Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA) according to manufacturer’s instructions. Serum creatinine was detected with (Enzymatic) Reagent Set from Pointe Scientific, Inc. (Canton, MI).
Cheng H., Fan X., Lawson W.E., Paueksakon P, & Harris R.C. (2015). Telomerase deficiency delays renal recovery in mice after ischemia reperfusion injury by impairing autophagy. Kidney international, 88(1), 85-94.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!