Pe anti mouse cd117

The TLR agonists Pam3Cys, polyI:C, LPS, flagellin, and R848 were purchased from Invivogen (San Diego, CA, USA); CpG-2006 and CpG-1826 from Invitrogen (Carlsbad, CA, USA) or Genomics Biosci & Tech (New Taipei, Taiwan); BMS-345541, collagenase II, collagenase IV, deoxyribonuclease, and anti-Flag M2 antibody from Sigma-Aldrich Co. (St. Louis, MO, USA); and HA.11, PE-anti-human CD117, PE- anti-mouse CD117, and PE-rat IgG2b κ isotype control antibody from BioLegend (San Diego, CA, USA); PE-anti-human CD133 antibody from Miltenyi Biotec (Bergisch Gladbach, Germany); PE-anti-mouse CD11b antibody from eBioscience (San Diego, CA, USA); anti-phospho-RelA (Alexa Fluor 647 conjugate) and rabbit IgG isotype control antibody from Cell Signaling (Beverley, MA, USA). The miR-205 mimics and control were synthesized by GeneDirex (Gueishan Township, Taiwan). Antibodies against human and mouse COMMD1 were purchased from Abnova (Taipei, Taiwan) and Proteintech (Chicago, IL, USA), respectively; human recombinant TNF-α, IL-1β, epidermal growth factor (EGF), and basic fibroblast growth factor (bFGF) from Peprotech (Rocky Hill, NJ, USA); and reagents for luciferase assay, from Promega (Medison, WI, USA).

Rabbit anti‐MRGPRX2 antibody (Cat# HPAD5520), Anti‐Dinitrophenyl (DNP) mouse IgE (Cat# D8406), DNP‐Human serum albumin (HSA), Bovine serum albumin, dimethyl sulfoxide, red blood cell lysis buffer, and p‐nitrophenyl‐N‐acetyl‐β‐D glucosaminide were from Sigma‐Aldrich (Darmstadt, Germany). Recombinant murine IL‐3 and SCF were purchased from PeproTech (NJ). The rabbit anti‐PAMP antibody (Cat# orb10050) was obtained from Biorbyt (Cambridge, UK). Alexa Fluor 488‐conjugated avidin, fetal bovine serum, RPMI1640, Pen Strep, and DAPI were from Thermo Fisher (CA). The following reagents and antibodies were from Biolegend (CA): PE anti‐mouse CD117(Cat# 105807), APC anti‐mouse FcεRIα (Cat# 134315), and APC anti‐mouse CD107a (Cat# 121613). PAMP9‐20 was from Sangon Biotech (Shanghai, China).