The von Frey plantar aesthesiometer (IITC, Woodland Hills, CA, USA) was used to measure mechanical withdrawal thresholds (MWT). Animals were placed separately in Plexiglas cages on a punching table for 15 min to allow acclimatization to the environment prior to testing. Each rat's left hind paw was stimulated three times at 5 min intervals during the formal examination. Paw withdrawal, flinching, or licking was regarded as positive behavior (29 (link)). Each value was recorded; MWT were represented by the mean values.
Von frey plantar aesthesiometer
Manufactured by IITC Life Science
Sourced in United States
The Von Frey plantar aesthesiometer is a laboratory equipment used to assess mechanical sensitivity in animal models. It consists of a series of calibrated filaments that apply a controlled amount of force to the plantar (sole) surface of the animal's paw. The device measures the threshold at which the animal responds to the mechanical stimulation, providing a quantitative assessment of tactile sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using von frey plantar aesthesiometer
1
Mechanical Withdrawal Threshold Measurement
2
Mechanical Withdrawal Threshold Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tests of the mechanical withdrawal threshold (MWT) were carried out to assess the response of the paw to mechanical stimulus. Mechanical hypersensitivity was determined with an electronic von Frey plantar aesthesiometer (IITC, Wood Dale, IL, USA). Baseline values were obtained prior to the STZ injection. Before the initial Von Frey measurements, rats were given 15 min to habituate to the test environment. A rigid tip was applied against the mid-plantar surface of the left hind paw. The paw withdrawal threshold was automatically recorded by the device, and the cut-off force was set at 60 g. The rigid tip was presented perpendicular to the plantar surface, and brisk withdrawal or paw flinching were considered as positive responses. Simultaneously, the digital number presented on the monitor was recorded as the MWT. Three successive stimuli were applied. The MWT for individual animals was represented by the mean values (calculated from the three successive stimuli).
3
Mechanical Withdrawal Threshold Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mechanical withdrawal thresholds (MWT) were assessed using an electronic von Frey plantar aesthesiometer (IITC, Woodland Hills, CA, United States). Baseline values were obtained before CCI surgery. The rats were placed individually in Plexiglas cages with a gridded floor for 30 min before the test to ensure acclimation. As described previously, the central part of the left hindpaw was stimulated, and paw withdrawal, flinching, or licking was considered to be a positive behavior (He et al., 2019 ). Three stimuli were applied at the same time point at intervals of 3 min. Each value was recorded, and MWT were represented by the mean values.
4
Measuring Mechanical Allodynia and Thermal Hyperalgesia in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The rats were placed in elevated perspex cages with a wire mesh oor and allowed to acclimatize for 30 min before the tests. To assess mechanical allodynia, the paw mechanical withdrawal threshold (MWT) was measured using an electronic von Frey plantar aesthesiometer (IITC, WoodDale, IL, USA) [22] . A rigid lament was applied perpendicularly to the mid-plantar surface of the right hind paw with increasing force. The paw withdrawal or paw inching was considered as a positive response and the pressure value recorded by the device was MWT. The test was repeated three times with an interval of 5 minutes and the average value was recorded as MWT for the rats. To assess thermal hyperalgesia, the thermal withdrawal latency (TWL) was tested using a radiant thermal stimulator (model 390; IITC Life Science Inc, Woodland Hills, CA). The radiant light was focused on the plantar surface of the right hind paw until the paw withdrawal was observed, and the time experienced is TWL. The test was repeated three times at intervals of at least 5 min and the average value was recorded as TWL for the rats.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!