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Reagent diluent 1

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Reagent diluent 1 is a laboratory product used to dilute reagents and samples for analysis. It is designed to maintain the stability and consistency of the diluted solution.

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Lab products found in correlation

Cicp and cic c1q, by Quidel (2 mentions) Pcsk9, by R&D Systems (2 mentions) Cxcl4, by R&D Systems (2 mentions) Chi3l1, by R&D Systems (2 mentions) 3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (2 mentions) Loxl2, by R&D Systems (2 mentions) Mmp 2, by R&D Systems (2 mentions) Pai 1, by R&D Systems (2 mentions) Mmp 9, by R&D Systems (2 mentions) Timp 1, by R&D Systems (2 mentions) Reagent diluent, by R&D Systems (1 mentions)

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Reagent Diluent

3 protocols using reagent diluent 1

1

Measurement of Fibrosis Biomarkers

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Levels of fibrosis biomarkers were measured on EDTA plasma by ELISA or multiplex assay using commercially available kits. HA (Echelon Biosciences, Salt Lake City, UT), LOXL2 (R&D Systems, Minneapolis, Minnesota), and CICP and CIC C1Q (Quidel, San Diego, CA) were measured by ELISA. PAI-1, PCSK9, TGF-β1-3, MMP-2, MMP-9, TIMP-1, CXCL4, Chi3L1 (all R&D Systems, Minneapolis, MN) were measured using Luminex xMAP technology. All analytes except LOXL2 were measured according to the manufacturers’ instructions. For LOXL2, the ELISA plates were coated overnight with capture antibody at room temperature. The plate was blocked with reagent diluent 1 (R&D Systems, Minneapolis, MN) for 1 hour and washed. Plasma was diluted 1:10 in PBS-Tween 10 and added to the plate. After incubating for 2 hours at 37° C and washing, detection antibody was added for 2 hours at 37° C. The plate was washed, streptavidin was added and the plate was incubated for 20 minutes at room temperature. After washing, TMB was added (Sigma-Aldrich Corp., St. Louis, MO), the plate was incubated for 20 minutes at room temperature, and sulfuric acid was added to stop the reaction. The plate was read at 450 nm.
Seang S., Somasunderam A., Nigalye M., Somsouk M., Schacker T.W., Sanchez J.L., Hunt P.W., Utay N.S, & Lake J.E. (2017). Circulating LOXL2 Levels Reflect Severity of Intestinal Fibrosis and GALT CD4+ T Lymphocyte Depletion in Treated HIV Infection. Pathogens & Immunity, 2(2), 239-252.
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2

Quantifying Mouse C3a Levels

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Plasma samples were prepared in 10 mM EDTA from whole blood collected by terminal cardiac puncture. Cells were removed from plasma by centrifugation. 96-well plates were coated with capture rat anti-mouse C3a antibody (BD Pharmingen, 558250) at 1 μg/µl in pH 6.5 PBS overnight at 4°C. Samples were diluted 1:100 in Reagent Diluent (Reagent Diluent 1, R&D Systems, DY997) and incubated overnight at 4°C. Biotin rat anti-mouse C3a antibody at 0.5 μg/µl (BD Pharmigen, 558251) was used, and analyzed by ELISA using the manufacturer’s protocol.
Kwak J.W., Laskowski J., Li H.Y., McSharry M.V., Sippel T.R., Bullock B.L., Johnson A.M., Poczobutt J.M., Neuwelt A.J., Malkoski S.P., Weiser-Evans M.C., Lambris J., Clambey E.T., Thurman J.M, & Nemenoff R.A. (2017). Complement activation via a C3a receptor pathway alters CD4+ T lymphocytes and mediates lung cancer progression. Cancer research, 78(1), 143-156.
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3

Quantifying Fibrosis Biomarkers in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Levels of fibrosis biomarkers were measured on EDTA plasma by ELISA or multiplex assay using commercially available kits. HA (Echelon Biosciences, Salt Lake City, UT), LOXL2 (R&D Systems, Minneapolis, Minnesota), and CICP and CIC C1Q (Quidel, San Diego, CA) were measured by ELISA. PAI-1, PCSK9, TGF-ß1-3, MMP-2, MMP-9, TIMP-1, CXCL4, Chi3L1 (all R&D Systems, Minneapolis, MN) were measured using Luminex xMAP technology. All analytes except LOXL2 were measured according to the manufacturers' instructions. For LOXL2, the ELISA plates were coated overnight with capture antibody at room temperature. The plate was blocked with reagent diluent 1 (R&D Systems, Minneapolis, MN) for 1 hour and washed. Plasma was diluted 1:10 in PBS-Tween 10 and added to the plate. After incubating for 2 hours at 37° C and washing, detection antibody was added for 2 hours at 37° C. The plate was washed, streptavidin was added and the plate was incubated for 20 minutes at room temperature. After washing, TMB was added (Sigma-Aldrich Corp., St. Louis, MO), the plate was incubated for 20 minutes at room temperature, and sulfuric acid was added to stop the reaction. The plate was read at 450 nm.
Seang S., Somasunderam A., Nigalye M., Somsouk M., Schacker T.W., Sanchez J.L., Hunt P.W., Utay N.S, & Lake J.E. (2017). Circulating LOXL2 Levels Reflect Severity of Intestinal Fibrosis and GALT CD4+ T Lymphocyte Depletion in Treated HIV Infection. Pathogens & Immunity, 2(2), 239-252.
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