PE-conjugated anti-CD127 (A7R34), FITC-conjugated ani-KLRG1 (2F1/KLRG1), V500-conjugated anti-CD4 (GK1.5), allophycocyanin (APC)-Cy7-conjugated anti-Thy1.2 (30-H12), PE-Cy7-conjugated anti-CD3e (145-2C11), PE-conjugated anti-CD62L (MEL-14), APC-conjugated anti-CD44 (IM7), biotinylated anti-T1/ST2 (RMST2-33), APC-Cy7-conjugated anti-c-KIT (2B8), PE-conjugated anti-I-A/I-E (major histocompatibility complex class II [MHC-II]) (clone M5/114.15.2), PerCP-Cy5.5-conjugated mAbs against lineage markers (CD11b [M1/70], CD11c [HL3], Gr.1 [RB6-8C5], CD49b [DX5], CD8 [53–6.7], B220 [RA3-6B2], CD335 [NKP46, 29A.4]) antibodies were purchased from e-Bioscience. Brilliant Violet 421-labeled Streptavidin was purchased from BioLegend, and anti-IL-17RB antibody (clone SS13B) obtained from Dr. Yui-Hsi Wang, University of Cincinnati, Cincinnati Children’s Hospital Medical Center) was conjugated with APC using Zenon Alexa Fluor 647 mouse IgG1 labelling Kit (Thermo Fisher Scientific).
Apc conjugated anti cd44
Manufactured by Thermo Fisher Scientific
Sourced in United States
The APC-conjugated anti-CD44 is a laboratory reagent used for the detection and analysis of CD44 expression on cells. CD44 is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. The APC (Allophycocyanin) fluorescent dye is conjugated to the anti-CD44 antibody, allowing for the identification and quantification of CD44-positive cells by flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 647 conjugated anti gl7, by BioLegend (2 mentions)
Pe conjugated anti cd62l, by BD (2 mentions)
Apc conjugated anti cd86, by Cytek Biosciences (2 mentions)
Pe conjugated anti pd 1, by Cytek Biosciences (2 mentions)
Efour450 conjugated anti cd127, by Thermo Fisher Scientific (2 mentions)
Pe conjugated anti cd25, by Cytek Biosciences (2 mentions)
Apc conjugated anti ctla4, by Cytek Biosciences (2 mentions)
Pe conjugated anti cd69, by BioLegend (2 mentions)
Macsquant flow cytometer, by Miltenyi Biotec (2 mentions)
Pe conjugated anti cd138, by BioLegend (1 mentions)
Fitc conjugated anti cd4, by Thermo Fisher Scientific (1 mentions)
Apc cy7 conjugated anti cd62l, by BioLegend (1 mentions)
Fitc conjugated anti cd25, by BD (1 mentions)
Ic fixation buffer, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti cd24, by BioLegend (1 mentions)
Facs buffer, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 conjugated anti cd4, by BD (1 mentions)
5 protocols using apc conjugated anti cd44
1
Multicolor Flow Cytometry Panel for Immune Cell Profiling
2
Multiparameter Flow Cytometry of Immune Cells
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The cells were analyzed on a MACSQuant Flow Cytometer (MiltenyiBiotec) using the following specific antibodies: VioletFluorTM 450-conjugated anti-B220 (TONBO Biosciences), Brilliant Violet 510TM-conjugated anti-CD4 (BioLegend), Brilliant Violet 510TM-conjugated anti-CD8a (BioLegend), phycoerythrin (PE)-conjugated anti-CD138 (BioLegend), Alexa Fluor 647-conjugated anti-GL7 (BioLegend), PE-conjugated anti-CD62L (BD Biosciences), allophycocyanin (APC)-conjugated anti-CD44 (eBioscience), APC-conjugated anti-CD86 (TONBO Biosciences), PE-conjugated anti-CD69 (BioLegend), PE-conjugated anti-CD23 (BioLegend), Alexa Fluor 647-conjugated anti-CD21 (BD Pharmingen), PE-conjugated anti-PD-1 (TONBO Biosciences), Brilliant Violet 421TM-conjugated anti-CXCR5 (BioLegend), Alexa Fluor 647-conjugated ICOS (BioLegend), eFour450-conjugated anti-CD127 (eBioscience), PE-conjugated anti-CD25 (TONBO Biosciences) and APC-conjugated anti-CTLA4 (TONBO Biosciences). Data analysis was conducted with FlowJo software (v7.6.5; FLOWJO, LLC).
3
Multiparametric Analysis of T Cell Subsets
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Samples were stained with the following antibodies: FITC-, eFluor450-, and APC-conjugated anti-CD8a (Clone 53-6.7, eBioscience, San Diego, CA, USA), FITC conjugated anti-CD4 (Clone GK1.5, eBioscience), APC/Cy7-conjugated anti-CD62L (Clone MEL-14, BioLegend, San Diego, CA, USA), APC-conjugated anti-CD44 (Clone IM7, eBioscience), Alexa Fluor 647- and eFluor 450-conjugated anti-CD3 (Clone 17A2, eBioscience), FITC- and PE-conjugated anti-Kb (Clone AF6-88.5.5.3, eBioscience), FITC-conjugated anti-CD25 (Clone 3C7, BD Pharmingen, USA), and FITC-conjugated Mouse Vβ TCR Screening Panel (BD Pharmingen).
4
Flow Cytometric Analysis of CD24/CD44 Expression
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The stably infected cells were collected and washed with PBS. Then, the cells were suspended in FACS buffer (00‐4222‐57, eBioscience) and stained with PE‐conjugated anti‐CD24 (311 105, BioLegend) and APC‐conjugated anti‐CD44 (338 805, eBioscience) at 4°C for 30 minutes. After staining, the cells were washed with PBS and resuspended in IC Fixation Buffer (00‐8222‐49, eBioscience) prior to FACS analysis.
5
Multiparameter Flow Cytometry Analysis of Immune Cell Phenotypes
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A MACSQuant Flow Cytometer (Miltenyi Biotec) was used to analyze whether the cells utilized the following specific antibodies: Violet Fluor™ 450-conjugated anti-B220 (Tonbo Biosciences); Alexa Fluor 647-conjugated anti-CD4 (BD Pharmingen); Brilliant Violet 510™-conjugated anti-CD4 (BioLegend); Brilliant Violet 510™-conjugated anti-CD8a (BioLegend); Alexa Fluor 647-conjugated anti-GL7 (BioLegend), phycoerythrin (PE)-conjugated anti-CD62L (BD Biosciences); allophycocyanin (APC)-conjugated anti-CD44 (eBioscience); APC-conjugated anti-CD86 (Tonbo Biosciences); PE-conjugated anti-CD69 (BioLegend); PE-conjugated anti-PD-1 (Tonbo Biosciences); Brilliant Violet 421™-conjugated anti-C-X-C chemokine receptor type 5 (BioLegend); Alexa Fluor 647-conjugated anti-inducible T cell co-stimulator (BioLegend); eFour450-conjugated anti-CD127 (eBioscience); PE-conjugated anti-CD25 (Tonbo Biosciences); and APC-conjugated anti-CTLA4 (Tonbo Biosciences). In addition, dead cells were excluded by propidium iodide staining. FlowJo (FlowJo, LLC) was used to conduct data analysis.
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