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Anti plc γ1 ptyr783

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Anti-PLC-γ1-pTyr783 is a laboratory reagent used to detect and quantify the phosphorylation of phospholipase C gamma 1 (PLC-γ1) at tyrosine 783. It is a highly specific primary antibody that can be used in various applications such as western blotting, immunoprecipitation, and immunohistochemistry to study cell signaling pathways involving PLC-γ1 phosphorylation.

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Lab products found in correlation

Anti zap70 ptyr319, by Cell Signaling Technology (2 mentions) Anti lat ptyr220, by Cell Signaling Technology (2 mentions) Anti slp 76 pser376, by Cell Signaling Technology (2 mentions) Anti relb, by Cell Signaling Technology (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti cd3ζ antibody, by Santa Cruz Biotechnology (1 mentions) Anti wnt3a, by Abcam (1 mentions) Anti pcna, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti zap70, by Cell Signaling Technology (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions) Anti plcγ1, by Cell Signaling Technology (1 mentions) Anti slp 76, by Cell Signaling Technology (1 mentions) Anti nck1 15b9, by Cell Signaling Technology (1 mentions) Superblock tbs, by Thermo Fisher Scientific (1 mentions) Western blocking reagent, by Merck Group (1 mentions) Anti lat, by Cell Signaling Technology (1 mentions) Anti lck d88, by Cell Signaling Technology (1 mentions)

2 protocols using anti plc γ1 ptyr783

1

Western Blot Analysis of Signaling Proteins

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Equal masses of protein lysates were loaded onto a 4–20% SDS-PAGE gel for electrophoresis. HM2 was used to detect GPC1 expression at 1 μg/ml. An anti-CD3ζ antibody was purchased from Santa Cruz Biotechnology (Cat#sc-166435, 1:200 dilution). Antibodies including anti-ZAP70-pTyr319 (Cat#2701S, 1:500 dilution), anti-LAT-pTyr220 (Cat#3584S, 1:500 dilution), anti-SLP-76-pSer376 (Cat#14745S, 1:500 dilution), anti-PLC-γ1-pTyr783 (Cat#2821S, 1:500 dilution), anti-RelA/p65-pSer536 (Cat#3033S, 1:500 dilution), anti-RelB (Cat#10544S, 1:500 dilution), anti-β-catenin (Cat#9582S, 1:1000 dilution), anti-β-actin (Cat#8457S, 1:2000 dilution), GAPDH (Cat#5174S, 1:5000 dilution), and anti-PCNA (Cat#2586S, 1:1000 dilution) were obtained from Cell Signaling Technology. Anti-Wnt3a was purchased from Abcam (Cat#ab28472, 1:100 dilution). Band intensities were quantified using ImageJ (NIH) and normalized to a mock sample.
Li N., Quan A., Li D., Pan J., Ren H., Hoeltzel G., de Val N., Ashworth D., Ni W., Zhou J., Mackay S., Hewitt S.M., Cachau R, & Ho M. (2023). The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer. Nature Communications, 14, 1986.
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2

Western Blot Analysis of T Cell Signaling Proteins

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Equal masses of protein lysate were loaded into 4–12% Bis-Tris NuPAGE Gels (ThermoFisher). After protein transfer onto nitrocellulose membranes (ThermoFisher), membranes were blocked with Western Blocking Reagent (Sigma) diluted 1:10 in 1× Tris-buffered saline (TBS). Membranes were stained with primary and secondary antibodies diluted 1:5,000–1:10,000 in SuperBlock TBS (ThermoFisher) supplemented with 0.1% Tween. The following antibodies were used: anti-CD247 (8D3, BD Biosciences), anti-CD247 pTyr142 (K25–407.69, BD Biosciences), anti-LAT (polyclonal, Cell Signaling), anti-LAT pTyr220 (polyclonal, Cell Signaling), anti-LCK (D88, Cell Signaling), anti-NCK1 (15B9, Cell Signaling), anti-PLC-γ1 (D9H10, Cell Signaling), anti-PLC-γ1 pTyr783 (D6M9S, Cell Signaling), anti-PLC-γ1 pSer1248 (D25A9, Cell Signaling), anti-SLP-76 (polyclonal, Cell Signaling), anti-SLP-76 pSer376 (D9D6E, Cell Signaling), anti-ZAP-70 (D1C10E, Cell Signaling), anti-ZAP-70 pTyr319 (65E4, Cell Signaling), anti-ZAP-70 pTyr493 (polyclonal, Cell Signaling), anti-mouse horseradish peroxidase (HRP) (polyclonal, Cell Signaling), and anti-rabbit HRP (polyclonal, Cell Signaling). Band intensities were quantified using ImageJ [National Institutes of Health (NIH)]; normalized to total protein or loading control, and then renormalized to a control sample.
Salter A.I., Rajan A., Kennedy J.J., Ivey R.G., Shelby S.A., Leung I., Templeton M.L., Muhunthan V., Voillet V., Sommermeyer D., Whiteaker J.R., Gottardo R., Veatch S.L., Paulovich A.G, & Riddell S.R. (2021). Comparative analysis of TCR and CAR signaling informs CAR designs with superior antigen sensitivity and in vivo function. Science signaling, 14(697), eabe2606.
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