Casp11

All protocols for animal care were approved by the Institutional Animal Care and Use Committee of the University of Pennsylvania. Unless otherwise specified, mice were 4 to 12 wk of age at the time of experimentation. While age and sex were controlled for each experiment, male and female mice were used indiscriminately for experiments as we have not seen a difference in infection. C57BL/6, Casp1/11^−/−, Asc^−/−, Casp11^−/−, Aim2^−/−, Nlrp1b^−/−, Nlrp3^−/−, and Il18^−/− mice were purchased from Jackson Laboratories. Nlrc4^−/−, Il1α^−/−, and Il1β^−/− mice were provided by Sunny Shin, University of Pennsylvania, Philadelphia, PA. Nlrp6^−/− mice were obtained from Maayan Levy, University of Pennsylvania, Philadelphia, PA, but originated from the laboratory of Richard Flavell, Yale University, New Haven, CT. Casp1^fl/fl and Casp1^fl/fl-vilCRE mice were bred in-house.

C57BL/6, Gsdmd^I105N/I105N (Kayagaki et al, 2015 (link)), Ninj1^−/− (Kayagaki et al, 2021 (link)) and Mefv^−/− (Feng et al, 2022 (link)) mice were sourced from The Australian National University. Nlrp3^−/− (Kovarova et al, 2012 (link)), Casp1^−/− (Rauch et al, 2017 (link)), Casp11^−/− (Wang et al, 1998 (link)) and Casp1/11^−/− (Kuida et al, 1995 (link)) mice were sourced from The Jackson Laboratory. Gsdmd^−/− (Kayagaki et al, 2015 (link)), Nlrc4^−/− (Mariathasan et al, 2004 (link)) and Asc^−/− (Mariathasan et al, 2004 (link)) mice were sourced from the University of Queensland. Aim2^−/− mice (Jones et al, 2010 (link)) were sourced from Genentech. Mlkl^−/− (Murphy et al, 2013 (link)) mice were sourced from The Walter and Eliza Hall Institute. All mice are on, or backcrossed to, the C57BL/6 background for at least 10 generation. Male and female mice of 6–8 weeks old were used. Mice were bred and maintained at The Australian National University under specific pathogen‐free conditions. All animal studies were conducted in accordance with the Protocol Number A2020/19 approved by The Australian National University Animal Ethics Committee.