Anti human pd l1 antibody

Manufactured by BioLegend

The Anti-human PD-L1 antibody is a laboratory reagent that can be used to detect the expression of the programmed death-ligand 1 (PD-L1) protein in human samples. PD-L1 is a key regulator of the immune response and is commonly expressed on various cell types, including tumor cells. This antibody can be utilized in various immunological assays, such as flow cytometry and immunohistochemistry, to analyze the presence and levels of PD-L1 in different research applications.

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Lab products found in correlation

Hrp labeled anti mouse igg, by Bio-Rad (1 mentions) Immulon 2 high binding 96 well plate, by Avantor (1 mentions) Recombinant human pd l1, by Abcam (1 mentions) Fortessa flow cytometer, by BD (1 mentions) Fitc conjugated anti mouse secondary antibody, by Merck Group (1 mentions) Facscan flow cytometer, by BD (1 mentions) Hpd l1, by BPS Biosciences (1 mentions) Anti human pd 1 antibody, by BioLegend (1 mentions) Hpd l1, by BioLegend (1 mentions) Facsaria, by BD (1 mentions)

Close spelling variants of this product

Phycoerythrin (PE)-labeled anti-human PD-L1 monoclonal antibody APC-conjugated anti-human PD-L1 antibody Anti-human PD-L1 Anti-PD-L1 antibody PD-L1 antibodies Anti-PD-L1 PD-L1

3 protocols using anti human pd l1 antibody

PD-L1 Binding Assay Protocol

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Immulon 2 high binding 96-well plate (VWR) was coated with 500 ng/well of recombinant human PD-L1 (BioVision). After blocking plate with PBS-T containing 3% BSA, serially diluted media of A549-infected with 1,000 vp/cell of HDegfp or HDPD-L1 mini were added and incubated at 4°C for 24 hours. Serially diluted anti-human PD-L1 antibody starting from 10 μg/well (BioLegend) was used as a positive control. After washing plate with PBS-T, HRP-labeled anti-human IgG for PD-L1 mini-body detection or HRP labeled anti-mouse IgG (BioRad) for anti-human PD-L1 and isotype antibody detection were added and incubated at room temperature for 1 hour and then we developed the washed plate. Absorbance was measured using Tecan reader (TECAN).

Tanoue K., Shaw A.R., Watanabe N., Porter C., Rana B., Gottschalk S., Brenner M, & Suzuki M. (2017). Armed oncolytic adenovirus expressing PD-L1 mini-body enhances anti-tumor effects of chimeric antigen receptor T-cells in solid tumors. Cancer research, 77(8), 2040-2051.

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Quantifying PD-L1 and DNA Damage in U2OS Cells

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U2OS cells were treated with doxycycline for 1–5 days and then harvested by trypsinization followed by washing with ice cold PBS and staining R-phycoerythrin conjugated anti-Human PD-L1 antibody (BioLegend Cat# 329706, RRID:AB_940368) for 30 minutes on ice. Flow cytometry analysis was performed on a BD LSR or Fortessa flow cytometer. Data are reported as the change in the MFI (mean fluorescence intensity) of PD-L1–isotype compared to isotype control. Alternatively, fixed cells were stained overnight with an anti-γH2AX antibody (JBW301, EMD Millipore, Cat# 05–636, RRID:AB_309864) followed by incubation with a FITC-conjugated anti-mouse secondary antibody (Sigma) as previously described (29 ). Samples were then stained with propidium iodide to assess total DNA content and analyzed on a FACScan flow cytometer (BD Biosciences) with FlowJo software (Tree Star, RRID:SCR_008520).

Zhao K., Zhang Q., Flanagan S., Lang X., Jiang L., Parsels L.A., Parsels J.D., Zou W., Lawrence T.S., Buisson R., Green M.D, & Morgan M.A. (2021). Cytidine Deaminase APOBEC3A Regulates PD-L1 Expression in Cancer Cells in a JNK/c-JUN-dependent Manner. Molecular cancer research : MCR, 19(9), 1571-1582.

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Generating Engineered Cell Lines for PD-1/PD-L1 Studies

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hPD-L1-TCR-HEK293T and TCR-HEK293T: One day before transfection, seed HEK293T cells at a density of 2x10⁶ cells per ml, when cells reached 80% confluent at the time of transfection. The next day, transfect 1 μl TCR activator and hPD-L1 (Cat#79455, BPS Bioscience) or the only TCR activator (Cat#79455, Cat#79455) into cells following the manufacturer’s protocol. To sort the hPD-L1-TCR -HEK293T cells, we stain the cells with anti-human PD-L1 antibody (Cat #329706, Biolegend) by the FACSAria (BD) after 3 days transfection.
hPD-1-NFAT-Jurkat cells: Lentiviral packaging of the plasmid pLenti-NFAT-IRES-EGFP-PD-1 were performed by Gene Pharma. Lentivirus were infected into Jurkat cells at MOI 20 with 2µg/ml Polybrene, 7 days post infection, hPD-1-NFAT-Jurkat cells were sorted by FACSAria (BD), with staining of anti- human PD1 antibody (Cat #329906, Biolegend). Single clones were performed and selected by expression of PD-1.

Wang Y., Gu T., Tian X., Li W., Zhao R., Yang W., Gao Q., Li T., Shim J.H., Zhang C., Liu K, & Lee M.H. (2021). A Small Molecule Antagonist of PD-1/PD-L1 Interactions Acts as an Immune Checkpoint Inhibitor for NSCLC and Melanoma Immunotherapy. Frontiers in Immunology, 12, 654463.

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