Male C57BL/6N mice (6–8 weeks) were purchased from Janvier (Le Genest, France) and treated according to the International Association for the Study of Pain guidelines. For all experiments the ethics guidelines for investigations in conscious animals were obeyed and the procedures were approved by the local ethics committee (Regierungspräsidium Darmstadt). The animals had free access to food and water and were maintained in climate‐ (23 ± 0.5°C) and light‐controlled rooms (light from 6.00 a.m. to 6.00 p.m.).
Inflammation was induced by injection of 10 μl zymosan (3 mg/ml in PBS; Merck, Darmstadt, Germany) subcutaneously into the plantar side of one hind paw. Eosinophil depletion was achieved by intraperitoneal (i.p.) injection of anti‐Siglec F antibody (0.883 mg/kg; clone 238,047; R&D Systems, Minneapolis, MN) 24 h prior zymosan injection. As control purified rat IgG2a (Biolegend, San Diego, USA) was used. IL‐4c was prepared using IL‐4 (Peprotech, Hamburg, Germany) and anti‐IL4 antibody (Biolegend, San Diego, USA) (Finkelman et al, 1993 (link); Milner et al, 2010 (link); Jenkins et al, 2011 (link)) and administered i.p. (0.166 mg/kg IL‐4 and 0.883 mg/kg anti‐IL4 antibody) 24 h prior zymosan injection. (Finkelman et al, 1993 (link); Milner et al, 2010 (link); Jenkins et al, 2011 (link)).
Inflammation was induced by injection of 10 μl zymosan (3 mg/ml in PBS; Merck, Darmstadt, Germany) subcutaneously into the plantar side of one hind paw. Eosinophil depletion was achieved by intraperitoneal (i.p.) injection of anti‐Siglec F antibody (0.883 mg/kg; clone 238,047; R&D Systems, Minneapolis, MN) 24 h prior zymosan injection. As control purified rat IgG2a (Biolegend, San Diego, USA) was used. IL‐4c was prepared using IL‐4 (Peprotech, Hamburg, Germany) and anti‐IL4 antibody (Biolegend, San Diego, USA) (Finkelman et al, 1993 (link); Milner et al, 2010 (link); Jenkins et al, 2011 (link)) and administered i.p. (0.166 mg/kg IL‐4 and 0.883 mg/kg anti‐IL4 antibody) 24 h prior zymosan injection. (Finkelman et al, 1993 (link); Milner et al, 2010 (link); Jenkins et al, 2011 (link)).