Mouse breederdiet 8626

Five months old male mice on a C57BL/6 background weighing less than 40
g (Jackson Laboratory, Bar Harbor, ME, USA) were used for this study. Animals
were housed at 21–24°C, on a reverse light:dark 14:10 hour
schedule. Control mice were fed rodent chow containing 10.4% fat (Mouse Breeder
Diet 8626, Teklad, Madison, WI, USA). The mice with AHF pancreatitis were fed
liquid diet (LD101A, Test Diet, Richmond, IN, USA) with added corn oil (3.3%,
33/1000 g) and supplementary lard given daily in a condiment dish (1 g). The
total fat content was ~57%. Alcohol in the liquid diet was increased
weekly from zero to 4%, 5%, and then 6%. The AHF fed mice were maintained at 6%
alcohol for the remainder of the study. Body weight was monitored weekly.

Male CPC;Apc mice (n = 9–10 per group) and male CDX2P9.5-G19Cre;Apc^flox/flox and CDX2P9.5-G22Cre;Apc^flox/flox mice (n = 3 per group) received the Teklad Mouse Breeder Diet 8626 from week 3 to the end of their life. Tap water (0.7 mg/L chlorinated water) was provided to the control group, and 10.0 mg/L chlorinated water was provided to the study group. CPC;Apc mouse were sacrificed at 40 weeks of age, and CDX2P9.5-G19Cre;Apc^flox/flox and CDX2P9.5-G22Cre;Apc^flox/flox mouse were at 15 weeks of age by cervical dislocation under anesthesia. The entire gastrointestinal tract was removed immediately after sacrifice and flushed with phosphate-buffered saline (PBS). Intestinal tissue was sliced longitudinally, and the location; multiplicity; and diameters of polyps in the small intestine, cecum, and colon were recorded. The intestine was transferred to 10% buffered formalin to be processed for histopathological studies. Consistent with the histologic appearance, a hemispherical shape was assumed for small and large bowel polyps (volume = 2/3πr³, r = radius).