Donkey anti Goat IR Dye 680RD (1: 10,000), 926-68074, Donkey anti Mouse IRDye 680RD (1: 10,000), Goat anti Rabbit IRDye 800CW (1: 10,000), 926-32211 (All Licor Biosciences Ltd), Goat anti Aconitase 2, Ab99467 (1: 10000), Rabbit anti Aconitase 2, Ab129069 (1: 50,000), Goat anti Aconitase 1, Ab10694 (1: 500), Complex II Ab Cocktail, Ab110410 (1: 250), Rabbit anti Glut4, Ab654 (1: 2,000), Rabbit anti SDHB, Ab84622 (1: 1,000), Rabbit anti TST, Ab60128, (1: 2,000)(All Abcam), Rabbit anti SDHB (1: 7,000), NBP1-54154, Rabbit anti SOD2 (1; 2,000), NBP1-40422, Goat anti SOD1 (1: 10,000), all Novus Biologicals, NB100-60944, Rabbit polyclonal anti-TST antibody, GTX 114858 (1: 1,000), Source Biochemicals.
Ab110410
Manufactured by Abcam
Sourced in United States
Ab110410 is an antibody that can be used for immunohistochemistry and western blotting applications. It is specific to the protein of interest.
Automatically generated - may contain errors
Lab products found in correlation
Ab84622, by Abcam (2 mentions)
Ab60128, by Abcam (2 mentions)
Nbp1 54154, by Novus Biologicals (2 mentions)
Nbp1 40422, by Novus Biologicals (2 mentions)
Ab10694, by Abcam (2 mentions)
Ab654, by Abcam (2 mentions)
Ab129069, by Abcam (2 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Survivin, by Cell Signaling Technology (1 mentions)
Ab56889, by Cell Signaling Technology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Ab186735, by Abcam (1 mentions)
Ab56788, by Abcam (1 mentions)
Ab57602, by Abcam (1 mentions)
Ab71498, by Abcam (1 mentions)
Ab42364, by Abcam (1 mentions)
Amersham imager 680, by GE Healthcare (1 mentions)
Sc 11415, by Santa Cruz Biotechnology (1 mentions)
Ab181873, by Abcam (1 mentions)
5 protocols using ab110410
1
Multiparameter Immunoblotting Analysis
2
Mitochondrial Dynamics Protein Analysis
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The primary antibodies used in the present study were as follows [30 (link)]: Drp1 (1:1000, Abcam, #ab56788), Fis1 (1:1000, Abcam, #ab71498), Opa1 (1:1000, Abcam, #ab42364), Mfn1 (1:1000, Abcam, #ab57602), Mfn2 (1:1000, Abcam, #ab56889), Mff (1:1000, Cell Signaling Technology, #86668), Bcl2 (1:1000, Cell Signaling Technology, #3498), Bax (1:1000, Cell Signaling Technology, #2772), caspase9 (1:1000, Cell Signaling Technology, #9504), survivin (1:1000, Cell Signaling Technology, #2808), p53 (1:1000, Cell Signaling Technology, #9282), complex III subunit core (CIII-core2, 1:1000, Invitrogen, #459220), complex II (CII-30, 1:1000, Abcam, #ab110410), complex IV subunit II (CIV-II, 1:1000, Abcam, #ab110268), Tom20 (1:1,000, Abcam, #ab186735) [31 (link)].
3
Multiparameter Immunoblotting Analysis
4
Immunoblotting Analysis of Cellular Signaling
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Cells were lysed in Lamelli buffer and samples were separated by SDS polyacrylamide gel electrophoresis. Nitrocellulose or PVDF membranes were probed with antibodies for BMAL1 (14020S, CST), TOM20 (sc-11415, Santa Cruz), β-Actin (4967S, CST) Pro-IL1β (AF-401-NA, CST), Complex II WB Antibody Cocktail (ab110410, Abcam) HIF-1α (14179, CST), GLUT1 (12939, CST), PDHα (3205S, CST), PKM2 (4053, CST), α-Tubulin (3873, CST), Histone H3 (4499, CST), and pSTAT3-Tyr705 (9145, CST). Bands were visualized using an Amersham 680 Imager (GE Healthcare). Densitometry quantification was performed according to the following protocol: http://www.yorku.ca/yisheng/Internal/Protocols/ImageJ.pdf .
5
Protein Expression Analysis by Western Blot
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Protein levels of interest were determined by Western blot analysis according to a protocol described elsewhere [20 (link)]. Cells were treated with NTG in the 6-well plate, and the whole-cell lysates were quantified with a BCA kit (P0012, Beyotime, Shanghai, China). The protein sample (30 μg/sample) was loaded in each lane of the gel. The primary antibodies to pAMPK (α1T183 + α2T172, ab133448), pPDE1a (Ser297, ab177461), Citrate synthase (ab129095), IDH2 (ab131263), MDH2 (ab181873), Complex I (NDUFB8, ab110242), Complex II (ab110410), Complex III (UQCRC2, ab14745), Complex IV (COX IV, ab16056) and β-tubulin (ab6046) were obtained from Abcam (Cambridge, MA, USA). Antibody to ATP5A1 (495240) was from Invitrogen (Camarillo, CA, USA). β-tubulin was used as the internal control. The signal was collected with a Chemiluminescence gel imager (Amersham Imager 600, GE Healthcare, Chicago, IL, USA) and the images were quantified with the Image J software.
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