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26 protocols using cf hq290i

1

Endoscopic Polyp Resection Protocol

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A conventional colonoscope (CF-HQ290I, PCF-Q260AZI, and PCF-H290I, Olympus Corporation, Tokyo, Japan; EC-L590ZW, Fujifilm Co., Ltd., Tokyo, Japan) was used for all colonoscopic examinations. When colorectal polyps or tumors were observed, endoscopic resection including polypectomy and endoscopic mucosal resection (EMR) was performed if possible without hospitalization. Most procedures were performed with air insufflation but CO2 insufflation in some cases. Conscious sedation was performed, mostly with midazolam (2–10 mg), according to the patient's request or pain status in previous procedures. Antispasmodics such as scopolamine butylbromide or glucagon were administered depending upon the colonoscopist's decision. Monitoring with an automatic blood pressure monitor was performed in all cases during sedation.
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2

Endoscope Comparison for Surgical Procedures

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PCF-H260I, PCF-Q260ZI, or PCF-H290ZI (Olympus Corporation; Tokyo, Japan) was used
in the SCE group; these have an endoscope diameter of 11.3 or 11.7 mm and a
3.2-mm working channel. CF-H260AI, CF-HQ290I, or CF-H290ZI (Olympus Corporation)
was used in the LCE group; these have an endoscope diameter of 13.2 mm and a
3.7-mm working channel.
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3

Intestinal Biopsies in Obesity and Diabetes

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Six male obese participants with type 2 diabetes, six one-year post-RYGB patients and six normal glycemic subjects were enrolled in the study. Anthropometric data are provided in Supplementary Table 1. Participation in this study was voluntary and written informed consent was obtained from each participant. The guidelines of the Declaration of Helsinki of the World Medical Association were followed. All protocols were approved by the Research Ethics Committee of the First Affiliated Hospital of Jinan University. All participants were fasted for 12 h. An enteroscopy was performed in sedated participants using a colonoscopy (CF-HQ290I; Olympus). Mucosal biopsies were taken from intestines. Tissue samples were extracted for protein and RNA or for immunohistochemistry, respectively.
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4

Colonoscopy Technique and Outcomes

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Thirty endoscopists with various levels of experience performed the colonoscopies. Endoscopists with adenoma detection rates ≥ 40 % were defined expert endoscopists
15 (link)
. A combination of the Elite system and CF-HQ290ZI, CF-HQ290I, PCF-H290ZI, or PCF-PQ260 L colonoscopes (Olympus Corporation) was used
16 (link)
. Withdrawal time was defined as the time required to examine the colorectal mucosa and remove the polyps
11
12
.
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5

Colonoscopy Systems and Techniques

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The CF260 group patients underwent colonoscopy using a high-definition endoscopy system with a high-definition colonoscope (CV-260 HiVision and CLV-260 with CF-H260AI, Evis Lucera Spectrum system; Olympus). Elite CF290 group patients underwent colonoscopy using a new-generation endoscopy system with a new-generation colonoscope (CV-290 and CLV-290, with CF-HQ290ZI, CF-HQ290I, or PCF-H290ZI, Evis Lucera Elite system; Olympus), a new-generation flushing pump (OFP-2; Olympus), and a carbon dioxide (CO
2) insufflator (UCR CO
2regulation unit; Olympus). PCF-H290ZI was used for patients aged 80 years or older, patients aged 70 years or older and who had undergone a previous abdominal surgery, and patients with difficult insertion due to a colon adhesion found during a previous colonoscopy. Narrow band imaging (NBI) is available for both the 260 series and 290 series.
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6

Comparative Evaluation of Olympus Colonoscopes

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The following colonoscopes were used: H260AI, Q260AZI, CF-Q260AI, CF-HQ290I, and HQ290I (Olympus, Tokyo, Japan).
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7

Endoscopic Mucosal Resection Bowel Prep

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The bowel preparation was initiated from the day prior to the EMR. Each patient was instructed to consume a low-residue diet and take 5 mg of oral sodium picosulfate on the evening before the procedure. On the day of the EMR, the patients received 2000 ml of polyethylene glycol (PEG). If the stools were not sufficiently clear, an additional 1000-2000 ml of PEG was given to ensure sufficient bowel cleaning. We used either conventional or magnifying endoscopes (CF-Q260AI, CF-H260AZI, PCF-Q260AZI, CF-HQ290I; Olympus, Tokyo, Japan). If necessary, midazolam was used for sedating the patient, and the cardiorespiratory function was monitored during the procedure. All procedures were performed with a CO2 insufflation system. The VIO300D (ERBE Elektromedizin, Tuebingen, Germany) power source was used for the electrical cutting and coagulation, and the polyp was removed with snares (Olympus) after saline injection mixed with indigo carmine. After the EMR, we carefully observed the wound in all cases using the white light and narrow-band imaging (NBI) endoscopy modes to confirm that there was no residual lesion.
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8

Colonoscopic Polypectomy Techniques

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Colonoscopic polypectomy was performed using an electric endoscope (CF‐HQ290I or CF‐H260AI; Olympus, Beijing, China). Generally, polypectomy was performed by endoscopic mucosal resection or a hot snare polypectomy using an electrosurgical device (VI0200S; ERBE China, Shanghai, China). Prophylactic clipping was routinely performed after polyps larger than 5 mm were resected by using the hot polypectomy, and hemostatic clipping or thrombin spraying was considered when delayed bleeding occurred. All procedures were performed by five experienced endoscopists, each of whom had performed more than 10 000 colonoscopies.
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9

Preoperative Diagnosis of Colorectal Lesions

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Preoperative diagnosis was performed by colonoscopy with magnifying function (CF-HQ290I, CF-H260AZI, PCF-Q240ZI, or PCF-Q260AZI; Olympus Medical Systems Corp., Tokyo, Japan). All lesions were subjected to pit pattern analysis by magnifying chromoendoscopy (MCE) with indigo–carmine dye (0.4 %) and narrow-band imaging (NBI) system. If type V pit pattern was suspected, we also performed estimation by MCE using crystal violet staining (0.05 %). Invasive pattern is used as an index for SM-d cancer [25 (link)].
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10

Colonoscopy Quality Evaluation Protocol

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Colonoscopies were completed with a standard colonoscope (CF H260AI or CF-HQ290I; Olympus) by experienced endoscopists who had experience performing more than 500 colonoscopies. The endoscopists aimed for a withdrawal time of ≥6 minutes according to the current quality indicators for colonoscopy. Air insufflation was used in all procedures. The detailed colonoscopy procedure was explained to each participant before the scheduled colonoscopy appointment. Polyethylene glycol (Klean-PrepR, Helsinn Birex Pharmaceuticals Ltd.) was used as the standardized bowel preparation. Before the procedure, all patients received a sedation regimen comprising midazolam 2.5 mg and meperidine 25 mg, and further doses were administered according to the needs of the participants. Bowel preparation was reported according to the Boston bowel preparation scale, and a preparation scale score ≥6 was considered good. The cecal intubation rates were also retrieved and summed.
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