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Annexin 5 fluorescein isothiocyanate propidium iodide fitc pi apoptosis detection kit

Manufactured by MultiSciences Biotech
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Sourced in China

The Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) apoptosis detection kit is a laboratory tool used to evaluate cell death and apoptosis. It employs the binding of Annexin V-FITC to phosphatidylserine and the use of propidium iodide to detect changes in cell membrane permeability, which are associated with the progression of apoptosis.

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Lab products found in correlation

Trizol plus rna purification kit, by Thermo Fisher Scientific (1 mentions) Facscan instrument, by BD (1 mentions) Pi rnase staining kits, by MultiSciences Biotech (1 mentions)

Close spelling variants of this product

Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis kit Annexin V-FITC/propidium iodide (PI) apoptosis detection kit Annexin V-FITC/propidium iodide (PI) apoptosis kit Annexin-V/Propidium Iodide (PI) apoptosis kit Annexin V/propidium iodide (PI; Annexin V-FITC Apoptosis Detection Kit Annexin V-fluorescein isothiocyanate (FITC) FITC Annexin V Apoptosis Kit Annexin V Apoptosis Detection Kit Annexin V-FITC/PI

2 protocols using annexin 5 fluorescein isothiocyanate propidium iodide fitc pi apoptosis detection kit

1

Apoptosis Detection and Gene Expression Analysis

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An Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) apoptosis detection kit was purchased from Multisciences (Lianke) Biotechnology (Hangzhou, China). Cell Counting Kit (CCK)8 detection kit and an enhanced chemiluminescence detection (ECL) kit were obtained from Beijing 4A Biotech (Beijing, China). TRIzol® Plus RNA Purification kit and MultiScribe RT kit were purchased from Invitrogen (Carlsbad, CA, USA). A SYBR Green RT-PCR kit (QPK-201) was from Toyobo (Tokyo, Japan). The primers used in the real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were provided by Shengon Biotech (Shanghai, China).
Xu Y., Chen W., Chen Z., Huang M., Yang F, & Zhang Y. (2021). Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway. Drug Design, Development and Therapy, 15, 753-767.
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2

Cell Cycle and Apoptosis Analysis

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Cells were harvested and washed with ice-cold phosphate-buffered saline (PBS). The PI/RNase staining kits (Multi-sciences, Shanghai, China) and an annexin V-fluorescein isothiocyanate propidium iodide (FITC/PI) apoptosis detection kit (Multisciences, Shanghai, China) were used to detect cell cycle and apoptosis according to the manufacturer’s protocol using a FACScan instrument (BD, Franklin Lakes, NJ, USA), respectively. Three independent experiments were performed.
Xu D., Chen Y., Yuan C., Zhang S, & Peng W. (2019). Long non-coding RNA LINC00662 promotes proliferation and migration in oral squamous cell carcinoma. OncoTargets and therapy, 12, 647-656.
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