Xylazine chloride

Drugs and reagents used in this study (and their sources) were as follows: Croton oil (≈100%), arachidonic acid (AA, ≥99%), phenol (≥99%), capsaicin (≥99%), ethyl phenylpropiolate (EPP, 98%), dexamethasone (Dexa, ≥97%), indomethacin (Indo, ≥99%), phosphoric acid (85%), rutin hydrate (≥94%), quercetin (≥95%), kaempferol (≥99%), apigenin (≥99%), apigenin7-O-β-d-glucoside (≥98%), luteolin (≥97%), luteolin 7-O-β-d-glucoside (≥98%), hexadecyltrimethylammonium bromide (≥99%), 3,3′,5,5′-tetramethylbenzidine dihydrochloride hydrate (≥98%), p-nitrophenyl-acetamide-µ-d-glucopyranoside (≥98%), sodium phosphate (96%), glycine (≥98.5%), and sodium citrate dehydrate (≥99%) (Sigma-Aldrich Co., St. Louis, MO, USA), dimethyl sulfoxide (≥99.7%), hexane (≥99%), ethyl acetate (≥99%), and acetone (≥99%) (Vetec Química Farm Ltda, Rio de Janeiro, RJ, Brazil), acetonitrile (≥99.9%) (Tedia, Brazil), and ketamine chloride (10%) and xylazine chloride (2%) (Syntec, Hortolândia, SP, Brazil).

Phenol, croton oil, histamine, capsaicin, and dexamethasone were acquired from Sigma-Aldrich^® Co. (St. Louis, MO, USA). Acetone, dichloromethane, and ethanol were obtained from Vetec^® Química Farm. Ltda (Rio de Janeiro, RJ, Brazil), while xylazine chloride and ketamine chloride were from Syntec^® (Hortolândia, SP, Brazil), and dexchlorpheniramine maleate was from EMS^® (Hortolândia, SP, Brazil). All other solvents and chemicals were of analytical grade.