Neurobasal a medium

The reagents that were used in the experiments are listed below. (1) Sigma-Aldrich, Saint Louis, MO, USA: Poly(ethyleneimine) solution (Cat. no. P3143), penicillin–streptomycin (Cat. no. P4333), L-Glutamine (Cat. No G85402). (2) Life Technologies, Grand Island, NY, USA: B-27 supplement (Cat. no. 17504044), Trypsin 2.5% (Cat. no. 15090046). (3) Molecular Probes, Eugene, OR, USA: Fura-2 AM (Cat. no. F1221). (4) Tocris Bioscience, Bristol, UK: UBP 310 (Cat. no. 3621), 5-Nonyloxytryptamine oxalate (Cat. No. 0901), WIN 55,212-2 mesylate (Cat. no. 1038) (5) Alomone Labs, Jerusalem, Israel: D-AP5 (Cat. no. D-145), NBQX (Cat. no. N-185). (6) Cayman Chemical, Ann Arbor, MI, USA: Bicuculline (Cat. no. 11727), HEMADO (Cat. No. 21015); (7) AppliChem, Darmstadt, Germany: EDTA (Cat. no. A5097), EGTA (Cat. no. A-0878). (8) Dia-M, Moscow, Russian Federation: HEPES (Cat. no. 3350). (9) Abcam, Cambridge, UK: N6-Cyclohexyladenosine (Cat. no. ab120472). (10) Paneco, Moscow, Russian Federation: Neurobasal-A medium.

The reagents used in the experiments are listed below. (1) Sigma-Aldrich, Saint Louis, MO, United States: Poly(ethyleneimine) solution (Cat. no. P3143), penicillin–streptomycin (Cat. no. P4333), L-Glutamine (Cat. No G85402), Retigabine (Cat. No. SML0325), XE-991 (Cat. no. X2254). (2) Life Technologies, Grand Island, NY, United States: B-27 supplement (Cat. no. 17504044), Trypsin 2.5% (Cat. no. 15090046). (3) Molecular Probes, Eugene, OR, United States: Fura-2 AM (Cat. no. F1221). (4) Cayman Chemical, Ann Arbor, MI, United States: Bicuculline (Cat. no. 11727), 1-naphthylacetyl spermine (NASPM) (Cat. no. 18453). (5) Tocris Bioscience, Bristol, United Kingdom: ATPA (Cat. no. 11–071-0). (6) AppliChem, Darmstadt, Germany: EGTA (Cat. no. A-0878). (7) Dia-M, Moscow, Russian Federation: HEPES (Cat. no. 3350). (8) Paneco, Moscow, Russian Federation: Neurobasal-A medium.