Cells were transfected with siRNA-Control or siRNA-SChLAP1 for 48 h. The apoptosis of cells (early and late apoptotic cells) was analyzed via flow cytometry (FACScalibur; BD Biosciences) using the Annexin V-FITC/PI kit (BD Biosciences). Cells were resuspended with the binding buffer and stained with the Annexin V-FITC at room temperature for 30 min followed by staining with PI in the dark for 10 min at room temperature. The cell apoptosis profile was analyzed using the CellQuest Software version 0.9.3.1 (BD Biosciences).
Cellquest software version 0
Manufactured by BD
Sourced in United States
CellQuest software version 0.9.3.1 is a data acquisition and analysis software for flow cytometry applications. It provides basic functionality for managing flow cytometry experiments and data.
Automatically generated - may contain errors
5 protocols using cellquest software version 0
1
Evaluating Apoptosis in siRNA-Treated Cells
2
Quantifying Apoptosis in Infected Cells
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The apoptosis rate of infected CC cells was detected by flow cytometry analysis. The infected CC cells were stained using an Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit (BD Biosciences, USA), and the results were calculated with a flow cytometer using the CellQuest software version 0.9.3.1 (BD Biosciences, USA).
3
Apoptosis Analysis via Annexin V-FITC/PI
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Cells were transfected with indicated plasmids (pcDNA/TP73-AS1) or negative control for 48 h as aforementioned. Cells were stained using an Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit (BD Biosciences, San Jose, CA, USA), according to the manufacturer's protocol. Cells were analyzed using a flow cytometer and CellQuest software version 0.9.3.1 (BD Biosciences). All experiments were performed in triplicate.
4
Apoptosis Analysis by Flow Cytometry
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Flow cytometry was performed to analyze the apoptosis of indicated cells. Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (Keygen, Nanjing, China) was used,following the manufacturer’s protocol. CellQuest software version 0.9.3.1 (BD Biosciences) was used to calculate the flow cytometry results.
5
Annexin V-FITC/PI Apoptosis Analysis
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To analyze apoptosis condition of indicated cells, flow cytometry analysis was performed. According to the user guide, an Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit (BD Biosciences, San Jose, CA, USA) was applied to stain cells. Apoptotic cells were analyzed by utilizing flow cytometer and CellQuest software version 0.9.3.1 (BD Biosciences).
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