Sw1990

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SW1990 is a laboratory centrifuge designed for general-purpose applications. It is capable of separating samples by centrifugal force.

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SW1990 (L-15, (3 citations)

45 protocols using sw1990

Cancer Cell Line Cultivation Protocol

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Colon cancer cell lines HCT116, LoVo, SW480, gastric cancer cell lines AGS, BGC823, MGC803, lung cancer cell lines A549, H1299, PG, esophageal cancer cell line EC9706, pancreatic cancer cell lines ASPC-1, PANC-1, SW1990, liver cancer cell lines BEL7402, HEPG2, SMMC7721 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Esophageal cancer cell KYSE30-lm3 and KYSE450-lm2 were gifted by Professor Zhihua Liu (Cancer Institute, Cancer Hospital, Chinese Academy of Medical Sciences). Primary human fibroblasts IMR90 was obtained from Cell Center, Chinese Academy of Medical Science.
HEPG2, PANC-1, SW1990, EC9706, KYSE30-lm3, KYSE450-lm2 and IMR90 were cultured in Dulbecco's modified Eagles medium (DMEM) (ThermoFisher Scientific, Inc.) with 10% fetal bovine serum (Biology industries). AGS, MGC803, BG823, HCT116, SW620, SW480, A549, H1299, PG, BEL7402 and SMMC7721 were cultured in RPMI-1640 medium (ThermoFisher Scientific, Inc.) with 10% fetal bovine serum.

Sun S., Meng L., Xing X., Li N., Song Q., Qiao D., Qu L., Liu C., An G., Li Z., Shou C, & Lian S. (2023). Anti-PRL-3 Monoclonal Antibody inhibits the Growth and Metastasis of colorectal adenocarcinoma. Journal of Cancer, 14(13), 2585-2595.

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Cancer Cell Line Cultivation Protocol

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Cultivation of Human Pancreatic Cell Lines

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Human pancreatic cancer cell lines Capan-2, CFPAC-1, SW1990, Mia-PaCa-2, PATU-8988, PANC-1, and Normal pancreatic duct cell line hTERT-HPNE were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Capan-2 cells were cultured in McCoy’s 5 A medium (Thermo Fisher Scientific). CFPAC-1 cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM, Thermo Fisher Scientific). SW1990, Mia-PaCa-2, PATU-8988, PANC-1, and hTERT-HPNE cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific). All media were supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), penicillin (100 U/mL), and streptomycin (100 µg/mL). Cells were incubated at 37 °C in an atmosphere of 5% CO₂. HEK-293 F cells were cultured in suspension with serum-free medium (Catalog No. 12338018; Thermo Fisher Scientific), and incubated at 37 °C in an atmosphere of 8% CO₂.

Wu G., Li L., Liu M., Chen C., Wang G., Jiang Z., Qin Y., He L., Li H., Cao J, & Gu H. (2022). Therapeutic effect of a MUC1-specific monoclonal antibody-drug conjugates against pancreatic cancer model. Cancer Cell International, 22, 417.

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Overexpression of RXRA and ALOX5 in Cancer Cells

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Human cancer cell lines (Capan-2, SW1990, H1299 and H460) were obtained from American Type Culture Collection (ATCC). All cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Cat. 11995040) supplemented with 10% Fetal Bovine Serum (FBS, Gibco, Cat. 10099–141) and maintained under an atmosphere containing 5% CO2 at 37 °C. All cell lines were negative for mycoplasma. Pancreatic cancer cell lines (Capan-2 and SW1990) were transfected with empty vector (EV), pcDNA3-RXRA WT, or pcDNA3-RXRA p.Ser427Phe, and lung cancer cell lines (H1299 and H460) were transfected with EV, pcDNA3-ALOX5 WT, or pcDNA3-ALOX5 p.Met146Lys using Lipofectamine 2000 (Invitrogen, Cat.11668019).

Cheng F., Zhao J., Wang Y., Lu W., Liu Z., Zhou Y., Martin W.R., Wang R., Huang J., Hao T., Yue H., Ma J., Hou Y., Castrillon J.A., Fang J., Lathia J.D., Keri R.A., Lightstone F.C., Antman E.M., Rabadan R., Hill D.E., Eng C., Vidal M, & Loscalzo J. (2021). Comprehensive characterization of protein-protein interactions perturbed by disease mutations. Nature genetics, 53(3), 342-353.

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Modulation of TUG1 and miR-29c in Pancreatic Cancer

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Human pancreas ductal epithelioid (HPDE) cells and four human pancreatic cancer cell lines (SW1990, AsPC-1, BxPC-3, and PANC-1) were purchased from the American Type Culture Collection. Cells were grown in RPMI-1640 medium (Invitrogen, CA, USA) supplemented with 10% fetal bovine serum (Gibco, CA, USA) and cultured in a 37°C humidified atmosphere of 5% CO₂. The knockdown and overexpression of TUG1 in BxPC-3 and PANC-1 cells were achieved by transfection with lentivirus vector containing TUG1 shRNA (forward, 5′-GATCCGCTTGGCTTCTATTCTGAATCCTTTCAAGAGAAGGATTCAGAATAGAAAGCCAAGCCAAGCTTTTTTG-3′; reverse, 5′-GCGAACCGAAGATAAGACTTAGGAAAGTTCTCTTCCTAAGTCTTATCTTCGGTTCGAAAAAAC-3′; GenePharma, Shanghai, China), The overexpression of TUG1 in SW1990 cells was achieved by transfection with the TUG1-pcDNA3.1 plasmid which constructed by Invitrogen (Invitrogen, CA, USA). Cells were transfected by Lipofectamine 2000 (Invitrogen, CA, USA). The overexpression and knockdown of miR-29c were performed using miR-29c mimic and miR-29c inhibitor (GeneCopoeia, Guangzhou, China), respectively. Cells transfected with empty vector or scramble control were used as negative control. Cells were plated in 6-well clusters or 96-well plates and transfected for 24 or 48 h. Transfected cells were used for further assays or protein extraction.

Lu Y., Tang L., Zhang Z., Li S., Liang S., Ji L., Yang B., Liu Y, & Wei W. (2018). Long Noncoding RNA TUG1/miR-29c Axis Affects Cell Proliferation, Invasion, and Migration in Human Pancreatic Cancer. Disease Markers, 2018, 6857042.

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Isolation and Polarization of Murine Macrophages

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Human PDAC cell lines PANC-1 and SW1990 were both purchased from American Type Culture Collection (Rockville, MD, USA). PANC-1 and SW1990 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 8% fetal bovine serum (FBS; Sigma-Aldrich, St Louis, MO, USA) in a humidified chamber with 5% CO₂ at 37 ℃. Mouse bone marrow was obtained and resuspended in DMEM to form a mononuclear white layer in 6-well plates (2×10⁶ cells/mL, 3.5 mL/well; Falcon, Corning, NY, USA). After 2 hours, cells were washed 2 times and adherent cells (monocytes) were kept culturing in DMEM with 10% heat-inactivated FBS supplemented with 20 ng/mL macrophage colony-stimulating factor (M-CSF) for 7–9 days. Half of the medium was refreshed every 2–3 days. The purity of adherent cells after 3 days was analyzed using a multicolor panel of primary monoclonal antibodies against CD3, CD86, and CD11b. For macrophage differentiation, M1 was induced by 20 ng/mL M-CSF and 30 ng/mL lipopolysaccharides (LPS); M2a was induced by 20 ng/mL M-CSF and 20 ng/mL IL-4; M2b was induced by 20 ng/mL M-CSF, 20 ng/mL LPS, and 50 µg/mL immune complex; M2c was induced by 20 ng/mL M-CSF, 20 ng/mL IL-10, and 20 ng/mL TGFβ1; and M2d was induced by 20 ng/mL M-CSF, 20 ng/mL IL-6, and 20 ng/mL toll-like receptor (TLR) ligands.

Gu H., Deng W., Zhang Y., Chang Y., Shelat V.G., Tsuchida K., Lino-Silva L.S, & Wang Z. (2022). NLRP3 activation in tumor-associated macrophages enhances lung metastasis of pancreatic ductal adenocarcinoma. Translational Lung Cancer Research, 11(5), 858-868.

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Culturing Human Pancreatic Cancer Cell Lines

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The human pancreatic cancer cell lines, including ASPC-1, SW1990, PANC-1, MIA Paca-2 and HPDE6-C7, were purchased from the University of Colorado Cancer Center Cell Bank. ASPC-1, PANC-1, MIA Paca-2 and HPDE6-C7 cells were cultured in DMEM medium with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO₂ atmosphere. SW1990 cells were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO₂ atmosphere. Cells were digested and passaged when cell confluence reached 80–90%.

Cui X.H., Hu S.Y., Zhu C.F, & Qin X.H. (2020). Expression and prognostic analyses of the insulin-like growth factor 2 mRNA binding protein family in human pancreatic cancer. BMC Cancer, 20, 1160.

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Molecular Mechanisms of Pancreatic Cancer

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The PC cell lines BxPC-3, AsPC-1, MIA PaCa-2, PANC-1, and SW1990 were acquired from the American Type Culture Collection. HPDE, AsPC-1, and BxPC-3 cells were cultivated in RPMI 1640 medium (Gibco, USA); MIA PaCa-2, PANC-1, and SW1990 cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA) including 1% penicillin and streptomycin. All the cells were cultured at 37° C in a 5% CO₂ incubator. FBXO28-overexpressing lentiviral vector (FBXO28), FBXO28 shRNA lentiviral vectors (sh-FBXO28#1 and sh-FBXO28#2), negative control lentiviral vectors (control and sh-NC), SMARCC2 plasmid (Myc-SMARCC2), and ubiquitin plasmid (HA-ub) were designed by Shanghai Jikai Biological Co. Lipofectamine 3000 (Invitrogen, USA) was used for transfection, and all steps were carried out in accordance with the instructions.

Liu S., Liu P., Zhu C., Yang R., He Z., Li Y., Li Y., Fei X., Hou J., Wang X, & Pan Y. (2023). FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination. Aging (Albany NY), 15(12), 5381-5398.

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Culturing Pancreatic Cell Lines

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One human normal pancreatic duct epithelial cell line (HPNE) and five pancreatic cancer cell lines (AsPC-1, PANC-1, BxPC-3, SW1990, and Capan-1) were purchased from the American Type Culture Collection (ATCC). HPNE, PANC-1, SW1990, and Capan-1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), penicillin (100 units/mL), and streptomycin (100 µg/ml). AsPC-1 and BxPC-3 cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum penicillin (100 units/mL), and streptomycin (100 µg/ml). Cells were incubated in a humidified atmosphere of 5% CO2 at a constant 37°C.

Huang W.K., Chen Y., Su H., Chen T.Y., Gao J., Liu Y., Yeh C.N, & Li S. (2021). ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway. International Journal of Biological Sciences, 17(7), 1808-1820.

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Culturing PC Cell Lines for EV Research

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Human PC cell lines SW1990 (CRL-2172) and PANC-1 (CRL-1469) were procured from American Type Culture Collection ([ATCC], Manassas, VA). Next, the CAFs, SW1990, and PANC-1 cells received culture in EV-free DMEM encompassing 10% FBS (A2720801, Gibco, with EVs removed), 1% penicillin, and 1% streptomycin in a humidified incubator with 5% CO₂ at 37°C (Thermo Fisher Scientific, Waltham, MA). Following culture, the medium was removed, followed by digestion with 0.25% trypsin for 2–5 min. Afterward, the CAFs, SW1990, and PANC-1 cells were resuspended, passaged, and cultured with 5 mL EVs-free DMEM encompassing 10% FBS (with EVs removed), 1% penicillin, and 1% streptomycin.

Han Y., Qian X., Xu T, & Shi Y. (2022). Carcinoma-associated fibroblasts release microRNA-331-3p containing extracellular vesicles to exacerbate the development of pancreatic cancer via the SCARA5-FAK axis. Cancer Biology & Therapy, 23(1), 378-392.

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