Wavefx spinning disk confocal system

Manufactured by Quorum Technologies

The WaveFX spinning disk confocal system is a specialized microscope that uses a spinning disk to illuminate and image samples. It allows for fast, high-resolution imaging of live cells and tissues. The system provides optical sectioning and confocal capabilities to enable 3D imaging and analysis.

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Lab products found in correlation

Plan fluor 20 objective, by Nikon (1 mentions) Imagem ccd camera, by Hamamatsu Photonics (1 mentions) Ix71 inverted microscope, by Olympus (1 mentions) Uplanfl n 10 objective, by Olympus (1 mentions) Metamorph software, by Molecular Devices (1 mentions) Eclipse ti microscope, by Nikon (1 mentions) Tmr star conjugated chitin binding probe, by New England Biolabs (1 mentions) Volocity 6.3 imaging software, by PerkinElmer (1 mentions) Volocity 3d image analysis software, by PerkinElmer (1 mentions) Fluorodish cell culture dish, by World Precision Instruments (1 mentions) Dm6000b inverted microscope, by Leica (1 mentions)

Close spelling variants of this product

WaveFX (21 citations) WaveFx spinning disk (4 citations) WaveFX spinning disk confocal microscope system (4 citations) Spinning disk confocal system (3 citations)

3 protocols using wavefx spinning disk confocal system

Multimodal Imaging of Cell Adhesion and Migration

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A WaveFX spinning disk confocal system (Quorum Technologies) equipped with an Eclipse Ti microscope (Nikon) and an ImagEM-CCD camera (Hamamatsu Photonics) was used for imaging the IF-stained coverslips and for recording cell migration when multiple channel acquisition was required. A Plan Fluor 40× objective (NA 1.3) was used for imaging the IF-stained coverslips. A Nikon 10× Ph1 ADL objective (NA 0.25) and a Plan Fluor 20× objective (NA 0.75) were used to image cell migration. DAPI, Alexa Fluor 488, Alexa Fluor 555, and acti-stain 670 were excited by laser at 405, 491, 561, and 642 nm, respectively (Semrock). Emission filters for those fluorophores were 460/50, 525/50, 593/40 or 620/60, and 700/75, respectively (Semrock). Images were acquired and analyzed with MetaMorph software (Molecular Devices).
TIRF microscopy and cell migration movies in phase contrast were performed using an inverted IX71 microscope (Olympus) with a Retiga EXi CCD camera (QImaging). An Olympus UPlanFl N 10× objective (NA 0.30) was used for cell-migration assays. TIRF images were taken with an Olympus PlanApo 60× oTIRFM objective (NA 1.45) and a 488-nm laser line from a HeNe laser (Prairie Technologies). MetaMorph software was used for image acquisition and analysis.

Erdogan B., Ao M., White L.M., Means A.L., Brewer B.M., Yang L., Washington M.K., Shi C., Franco O.E., Weaver A.M., Hayward S.W., Li D, & Webb D.J. (2017). Cancer-associated fibroblasts promote directional cancer cell migration by aligning fibronectin. The Journal of Cell Biology, 216(11), 3799-3816.

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Quantifying Fungal Cell Wall Chitin

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Fungi on coverslips were incubated with 50 μL of a chitin probe solution (TMR-Star Conjugated Chitin-binding Probe, a gift from New England Biolabs Ltd.) (20 μg/mL in PBS) overnight in the dark at 4°C. Coverslips were rinsed with PBS, dried and processed as described for α(1,3)-glucan staining. Fluorescence was visualized on a spinning disk confocal microscope (WaveFX spinning disk confocal system, Quorum Technologies) and signals were quantified using Volocity 6.3 imaging software (PerkinElmer). For each strain/polysaccharide, at least 30 images were analyzed and exposure was controlled manually at 900 ms. Control samples were processed in parallel except that the primary antibody or chitin probe was not added.

Nesbitt J.R., Steves E.Y., Schonhofer C.R., Cait A., Manku S.S., Yeung J.H., Bennet A.J., McNagny K.M., Choy J.C., Hughes M.R, & Moore M.M. (2018). The Aspergillus fumigatus Sialidase (Kdnase) Contributes to Cell Wall Integrity and Virulence in Amphotericin B-Treated Mice. Frontiers in Microbiology, 8, 2706.

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Imaging Zebrafish Embryo Development

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Embryos were collected for 30 min, then dechorionated using 5% bleach for 1 min and rinsed with water. Embryos were then aligned in a Fluorodish cell culture dish (World Precision Instruments) and covered with Halocarbon oil 400. Live imaging was performed using a WaveFX spinning disk confocal system (Quorum) and a DM6000B inverted microscope (Leica). Images were captured and processed using Volocity 3D image analysis software (PerkinElmer).

Dehghani M, & Lasko P. (2015). In vivo mapping of the functional regions of the DEAD-box helicase Vasa. Biology Open, 4(4), 450-462.

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