These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic heterozygous CamKIIa-Cre mice (Jackson Laboratories; CamKIIa-Cre B6.Cg-Tg(Camk2a-cre)T29-1Stl/J, stock number 005359, RRID: IMSR_JAX:005359; maintained on a C57BL/6J background). Mice were housed with their littermates until the surgical procedure with free access to food and water in a room with a 12/12h light/dark cycle, 19–23°C ambient temperature and 40–70% humidity. All mice held in IVC's, with wooden chew stick and nestlets. Mice were 4-7 months old at the time of testing. Experimental procedures performed on mice in accordance with the Animals (Scientific Procedures) Act, 1986 (United Kingdom), with final ethical review by the Animals in Science Regulation Unit of the UK Home Office.
Camkiia cre b6 cg tg camk2a cre t29 1stl j
Manufactured by Jackson ImmunoResearch
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CamKIIa-Cre B6.Cg-Tg(Camk2a-cre)T29-1Stl/J is a genetically modified mouse line that expresses Cre recombinase under the control of the calcium/calmodulin-dependent protein kinase II alpha (CaMKIIα) promoter. The Cre recombinase is a site-specific DNA recombinase that can be used to induce genetic modifications in target cells or tissues.
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3 protocols using camkiia cre b6 cg tg camk2a cre t29 1stl j
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Behavioral Experiments in C57BL/6J Mice
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Behavioral Experiments in C57BL/6J Mice
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These experiments used adult male C57BL/6J mice (Charles River Laboratories, UK) or transgenic heterozygous CamKIIa-Cre mice (Jackson Laboratories; CamKIIa-Cre B6.Cg-Tg(Camk2a-cre)T29-1Stl/J, stock number 005359, RRID: IMSR_JAX:005359; maintained on a C57BL/6J background). Mice were housed with their littermates until the surgical procedure with free access to food and water in a room with a 12/12h light/dark cycle, 19–23°C ambient temperature and 40–70% humidity. All mice held in IVC's, with wooden chew stick and nestlets. Mice were 4-7 months old at the time of testing. Experimental procedures performed on mice in accordance with the Animals (Scientific Procedures) Act, 1986 (United Kingdom), with final ethical review by the Animals in Science Regulation Unit of the UK Home Office.
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Neurobehavioral Protocols in Mice and Rats
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Animals used were male adult (4–7 months old) C57BL/6J mice (Charles River, UK) or transgenic heterozygous Cre-driver mice (Jackson Laboratories; obtained from C57BL/6J crossed with CamKIIa-Cre B6.Cg-Tg(Camk2a-cre)T29-1Stl/J, stock number 005359, RRID: IMSR_JAX:005359; or Dat-Cre B6.SJL-Slc6a3tm1.1(cre)Bkmn/J, stock number 006660, RRID: IMSR_JAX:006660). In addition, we used adult male Long-Evans rats (Harlan, UK) to test rat dorsal hippocampal CA1 LFPs for the presence of the theta-nested spectral components we identified in mice. All animals had free access to water and food in a dedicated housing facility with a 12/12 h light/dark cycle. They shared a cage with their littermates until the surgery. All experiments involving animals were conducted according to the UK Animals (Scientific Procedures) Act 1986 under personal and project licenses issued by the Home Office following ethical review.
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