MHCC97H—Control, MHCC97H-TNFAIP1, SMMC7721-shControl and SMMC7721-shTNFAIP1 stable cell lines were seeded into 24-well culture plate with glass covers. After 48 h, cells were fixed with 4% formaldehyde and subjected to permeabilization with 0.2% Triton X-100. The cells blocked with 1% BSA and incubated with the indicated antibodies which were shown in Supplementary Table S2, followed by marking nuclei with DAPI (Beyotime Biotechnology, China). Images were captured using Diaphot Inverted Microscope Camera System (Leica) [24] (link).
Diaphot inverted microscope camera system
Manufactured by Leica
The Diaphot Inverted Microscope Camera System is a versatile laboratory equipment designed for microscopic imaging. It features an inverted optical configuration, allowing for the examination of samples from below. The system includes a camera module that enables the capture and digital recording of microscopic observations.
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Lab products found in correlation
2 protocols using diaphot inverted microscope camera system
1
Immunofluorescence Staining of Cell Lines
2
Tubulogenesis Assay of Endothelial Cells
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The tube formation assay was performed by keeping 24-well culture plates on ice and coated with 200 μl of chilled Corning Matrigel Matrix (BD Biosciences, USA) per well. Incubate plates at 37 °C for 30 min, where it would polymerize. A volume of 200 μl of 1.2 × 105 cells of HUVEC—Control, HUVEC-TNFAIP1, HUVEC-shControl, HUVEC-shTNFAIP1 stable cells were seeded onto matrigel-coated plates. After 16 h, tubules were captured using Diaphot Inverted Microscope Camera System (Leica) and the length and the number of branch points was quantified for using NIH Image J software.
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