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Rat anti brdu antibody

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Sourced in Denmark

The Rat anti-BrdU antibody is a laboratory reagent used to detect the presence of bromodeoxyuridine (BrdU) incorporation in cells. BrdU is a thymidine analog that is incorporated into the DNA of cells during DNA synthesis, allowing for the identification of proliferating cells. The Rat anti-BrdU antibody specifically binds to the incorporated BrdU, enabling researchers to visualize and quantify cell proliferation using techniques such as immunohistochemistry or flow cytometry.

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Lab products found in correlation

Mouse anti neun antibody, by Merck Group (5 mentions) Vectashield mounting medium, by Vector Laboratories (5 mentions) Fitc conjugated anti mouse antibody, by Jackson ImmunoResearch (4 mentions) Cy3 conjugated goat anti rat antibody, by Jackson ImmunoResearch (4 mentions)

Close spelling variants of this product

Anti-BrdU antibody BrdU antibody Anti-BrdU Anti-TM

5 protocols using rat anti brdu antibody

1

Identifying Neurogenesis in Traumatic Brain Injury

Cited 1 time
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Newly generated neurons in the DG were identified by double labeling for BrdU and NeuN after TBI [43] (link). Briefly, after being deparaffinized and rehydrated, tissue sections were boiled in 10 mM citric acid buffer (pH 6) for 10 min. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 min. Sections were incubated with 1% BSA containing 0.3% Triton-X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1∶200; Chemicon, Temecula, CA) at 4°C overnight. For negative controls, primary antibodies were omitted. FITC-conjugated anti-mouse antibody (1∶400; Jackson ImmunoResearch, West Grove, PA) was added to sections at room temperature for 2 h. Sections were then incubated with rat anti-BrdU antibody (1∶200; Dako, Glostrup, Denmark) at 4°C overnight. Sections were then incubated with Cy3-conjugated anti-rat antibody (1∶400; Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 h. Each of the steps was followed by three 5-min rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector laboratories, Burlingame, CA).
Meng Y., Chopp M., Zhang Y., Liu Z., An A., Mahmood A, & Xiong Y. (2014). Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats. PLoS ONE, 9(9), e106238.
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2

Quantifying Neurogenesis and Angiogenesis Post-Injury

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Double immunostaining was performed to identify newly generated endothelial cells (BrdU/EBA+) and newly formed mature neurons (BrdU/NeuN+) 35 days after injury. Briefly, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% bovine serum albumin containing 0.3% Triton X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon) or anti-EBA at 4°C overnight. For negative controls, primary antibodies were omitted. Fluorescein isothiocyanate–conjugated anti–mouse antibody (1:400; Jackson ImmunoResearch) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako) at 4°C overnight, and subsequently incubated with Cy3-conjugated goat anti–rat antibody (1:400; Jackson ImmunoResearch) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector Laboratories).
Zhang Y., Zhang Z.G., Chopp M., Meng Y., Zhang L., Mahmood A, & Xiong Y. (2016). Treatment of traumatic brain injury in rats with N-acetyl-seryl-aspartyl-lysyl-proline. Journal of neurosurgery, 126(3), 782-795.
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3

Immunohistochemical Analysis of Post-TBI Neurogenesis

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Newly generated endothelial cells and newborn mature neurons in the lesion boundary zone and dentate gyrus 35 days after TBI were identified by double labeling for BrdU with EBA or NeuN, respectively. Briefly, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% BSA containing 0.3% Triton-X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon, Temecula, CA) or anti-EBA at 4°C overnight. For negative controls, primary antibodies were omitted. FITC-conjugated anti-mouse antibody (1:400; Jackson ImmunoResearch, West Grove, PA) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako, Glostrup, Denmark) at 4°C overnight. Sections were then incubated with Cy3-conjugated goat anti-rat antibody (1:400; Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector laboratories, Burlingame, CA).
Zhang Y., Chopp M., Meng Y., Katakowski M., Xin H., Mahmood A, & Xiong Y. (2015). Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury. Journal of neurosurgery, 122(4), 856-867.
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4

Identifying Neurogenesis after Traumatic Brain Injury

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Newly generated endothelial cells and newborn mature neurons in the lesion boundary zone and dentate gyrus 35 days after TBI were identified by double labeling for BrdU with EBA or neuronal nuclei (NeuN), respectively. In brief, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% BSA containing 0.3% Triton-X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon, Temecula, CA) or anti-EBA at 4°C overnight. For negative controls, primary antibodies were omitted. FITC-conjugated anti-mouse antibody (1:400; Jackson ImmunoResearch, West Grove, PA) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako, Glostrup, Denmark) at 4°C overnight. Sections were then incubated with Cy3-conjugated goat anti-rat antibody (1:400; Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector laboratories, Burlingame, CA).
Zhang Y., Chopp M., Zhang Z.G., Katakowski M., Xin H., Qu C., Ali M., Mahmood A, & Xiong Y. (2016). Systemic administration of cell-free exosomes generated by human bone marrow derived mesenchymal stem cells cultured under 2D and 3D conditions improves functional recovery in rats after traumatic brain injury. Neurochemistry international, 111, 69-81.
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5

Dual Labeling of Newborn Neurons in DG

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Double fluorescent staining with NeuN and BrdU was performed to identify newborn mature neurons in the DG. Briefly, after being deparaffinized and rehydrated, brain sections were boiled in 10 mM citric acid buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated in 2.4 N HCl at 37°C for 20 minutes. Sections were incubated with 1% BSA containing 0.3% Triton-X-100 in PBS. Sections were then incubated with mouse anti-NeuN antibody (1:200; Chemicon) at 4°C overnight. For negative controls, primary antibodies were omitted. FITC-conjugated antimouse antibody (1:400; Jackson ImmunoResearch, West Grove, PA) was added to sections at room temperature for 2 hours. Sections were then incubated with rat anti-BrdU antibody (1:200; Dako) at 4°C overnight. Sections were then incubated with Cy3-conjugated goat antirat antibody (1:400; Jackson ImmunoResearch) at room temperature for 2 hours. Each of the steps was followed by three 5-minute rinses in PBS. Tissue sections were mounted with Vectashield mounting medium (Vector laboratories).
Zhang Y., Chopp M., Zhang Z.G., Zhang Y., Zhang L., Lu M., Zhang T., Winter S., Doppler E., Brandstäetter H., Mahmood A, & Xiong Y. (2019). Cerebrolysin Reduces Astrogliosis and Axonal Injury and Enhances Neurogenesis in Rats After Closed Head Injury. Neurorehabilitation and neural repair, 33(1).
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