Gc ms 5975

To remove interfering matrices, the samples were first cleaned up using sample cleanup procedure, the analyte concentrated and the sample matrix changed to GC grade before analysis. Briefly, the solid phase extraction procedure employed C₁₈ cartilage conditioned with 3 mL of methanol then 3 mL of sample was loaded to allow it flow slowly out of the cartilage giving it enough time to interact with adsorbent. The sample was then allowed to dry in a stream of air for 10 min and thereafter eluted with 3 mL methanol into a 4 mL vial, concentrated using genetic concentrator, reconstituted with 1 mL of methanol, filtered using nylon micro filters size 0.22 μM into 1.5 mL vials and taken to GC-MS for analysis. GC–MS analysis of crude T. peruviana was evaluated using a shimadzu GC–MS at JKUAT, Analytical laboratory. 5 g of the powdered plant samples was extracted with Acetonitrile, then solvent exchanged with 2, 2, 4-Trimethylpentane before GCMS analysis. GC–MS technique was used for identification of the chemical compounds present in the extracts and it was carried out on Agilent 5975 GC–MS operating in EI mode at 70 eV with a mass range of 40–400 m/z. A capillary column 30 m × 0.25 mm (id) and Helium gas was used as carrier gas with flow rate of 1.2 mL/min and oven temperature of 60 °C [27 (link)].

The active ethyl acetate crude was subjected to GC-MS analysis on GC-MS- 5975 (AGILENT), column DB 5 ms Agilent, dimension length- 30.0 m, ID- 0.2 mm, flim thickness- 0.25 μm with temperature program- 70–300°C, 10°C/min, injection temperature- 240°C, carrier gas- helium, flowrate- 1.51 ml/min, equipped with GC-MS NIST-II library.