After flow cytometry analysis, the cells were collected for lysis. Protein concentration was determined using Keygen BCA Protein Assay Kit (KEYGEN, China). An equivalent amount of 20 μg protein was disintegrated using 10% SDS-PAGE and then transferred to the PVDF membrane. The membrane was blocked with 5% blocking buffer (5% skimmed milk powder + TBST) for 60 min at room temperature. Subsequently, the membrane was incubated with primary antibodies, including Caspase-3 antibody (Cell Signaling Technology, CST#9662S; 1:1,000), Caspase-7 antibody (CST#12827S; 1:1,000), and Caspase-8 antibody (CST#4790S; 1:1,000), overnight at 4 °C. The secondary antibodies were then added on the second day and incubated for 60 min. After the membranes were washed, the bands were visualized using ChemiDoc TMXRS+ (Bio-RAD, USA). The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013).
Caspase 7 antibody
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
The Caspase-7 antibody is a lab equipment product that can be used to detect the presence and expression levels of the Caspase-7 protein. Caspase-7 is a member of the caspase family of proteases, which play a crucial role in the process of apoptosis, or programmed cell death.
Automatically generated - may contain errors
Lab products found in correlation
Caspase 3 antibody, by Cell Signaling Technology (2 mentions)
β actin antibody, by Cell Signaling Technology (2 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
Caspase 8 antibody, by Cell Signaling Technology (1 mentions)
Nrf2 antibody, by Cell Signaling Technology (1 mentions)
Myc tag antibody, by Cell Signaling Technology (1 mentions)
α tubulin antibody, by Cell Signaling Technology (1 mentions)
Ho 1 antibody, by Cell Signaling Technology (1 mentions)
Nqo1 antibody, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 7 antibody, by Cell Signaling Technology (1 mentions)
Polyvinylidene difluoride membrane, by Roche (1 mentions)
Sds page, by Bio-Rad (1 mentions)
Anti rabbit igg secondary antibody, by Cell Signaling Technology (1 mentions)
3 protocols using caspase 7 antibody
1
Caspase Activation Analysis by Western Blot
2
Apoptosis and Oxidative Stress Regulation
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The following reagents were used: mouse IRG1 enzyme‐linked immunosorbent assay (ELISA) Kit (no. MBS9319749) from MyBioSource; cleaved caspase‐3 antibody (no. 9664S), caspase‐3 antibody (no. 9662S), cleaved caspase‐7 antibody (no. 9491S), caspase‐7 antibody (no. 9491T), Nrf2 antibody (no. 12721S), HO‐1 antibody (no. 82206S), Myc‐Tag antibody (no. 2276S), β‐actin antibody (no. 8457S), α‐tubulin antibody (no. 2144S), and lamin B2 antibody (no. 13823) from Cell Signaling Technologies; NQO1 antibody (no. sc‐32793) from Santa Cruz Biotechnology; and Nrf2 antibody (no. ab137550) and HO‐1 antibody (no. ab13248) from Abcam.
3
Western Blot Analysis of Akt2 and Caspase-7
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The Western blot analysis was performed as described earlier [16 (link)]. Briefly, proteins were harvested for Western blot analysis 48 h after transfection. After denatured at 100°C for 5 min, samples were separated (30 μg per lane) on 10% SDS-PAGE (Bio-Rad Laboratories, Hercules, CA, USA) and then transferred onto a polyvinylidene difluoride membrane (Roche Applied Science, Mannheim, Germany). The membrane was blocked with 5% skim milk at room temperature for 1 h, incubated overnight at 4°C with Akt2 antibody (abcam, Cambridge, UK) and caspase-7 antibody (Cell Signaling Technology, MA), then incubated with anti-rabbit IgG secondary antibody (Cell Signaling Technology, MA). A β-Actin antibody was used to determine loading (Cell Signaling Technology, Danvers, MA, USA). Antibody binding was visualized by chemiluminescence (Boster Company, Wuhan, China).
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