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14 protocols using cobalt 2 nitrate

1

Synthesis and Characterization of Metal-Organic Frameworks

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All analytical grade chemicals were used without further purification. Chromium(iii) nitrate (Cr(NO3)3·9H2O, 98%), terephthalic acid (H2BDC, 99%), Na2WO4·2H2O, H3PO4, cobalt(ii) nitrate (Co(NO3)2·6H2O, 98%), sodium hydroxide (NaOH, 99%), iron(iii) nitrate (Fe(NO3)3·9H2O, 98%), DMF and ethanol (C2H5OH, 99%) were obtained from Sigma-Aldrich. Methyl orange (MO, C14H14N3NaO3S, 98%), methylene blue (MB, C16H18ClN3S, 98%), hydrochloric acid (HCl, 36%) and rhodamine B (RhB, C28H31ClN2O3, 98%) were provided by Merck.
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2

Electrochromic Device Fabrication

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ITO/PET sheet (60 Ω cm−1), alizarin red S (3,4-dihydroxy-9,10-dioxo-2-anthracensulphonic acid, sodium salt), aluminium(III) nitrate (Al(NO3)3), Cobalt(II) nitrate (Co(NO3)2), Ni(II) nitrate (Ni(NO3)2, Ca(II) nitrate (Ca(NO3)2), Sr(II) nitrate (Sr(NO3)2), Cu(II) nitrate (Cu(NO3)2), acetate/sodium carbonate buffers and potassium chloride (KCl) were obtained from Sigma Aldrich Co.
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3

Structural, Compositional, and Magnetic Characterization of Transition Metal Nitrates

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A Rigaku Benchtop Miniflex X-ray diffraction (XRD) diffractometer (Tokyo, Japan) with Cu Kα radiation at room temperature (RT) over the 2θ range from 20° to 70° was used for the structural analysis. Scanning electron microscopy (SEM, FEI Titan ST, Hillsboro, OR, USA) along with energy dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM; FEI, Morgagni 268, Prague, Czech Republic) were used for the morphological and composition analyses. Spectral analysis of all products was performed via Fourier transform infrared (FT-IR) spectroscopy (Bruker, Berlin, Germany). Ultraviolet-visible (UV-vis) diffuse reflectance (DR%, Shimadzu, Tokyo, Japan) spectra were recorded in the 200 to 800 nm wavelength range using a DR spectrophotometer. The magnetic properties of the products were measured using a Quantum Design SQUID-PPMS vibrating sample magnetometer (PPMS DynaCool, Quantum Design, San Diego, CA, USA).
Cobalt (II) nitrate (Co(NO3)2), nickel (II) nitrate (Ni(NO3)2), iron (III) nitrate hexahydrate (Fe(NO3)3.6H2O), and niobium (V) nitrate (Nb(NO3)5) were received from Sigma-Aldrich (St. Louis, MO, USA) and used as received.
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4

Bioactive Glass-Coated Titanium Preparation

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Titanium discs (c.p. grade 2, Sigma Aldrich, St. Louis, MO, USA), tetraethyl ortho-silicate (Sigma Aldrich), calcium nitrate tetrahydrate (Sigma Aldrich), triethyl phosphate (Sigma Aldrich), silver nitrate (AR grade, Accu-Chem, Melrose Park, IL, USA), cobalt (II) nitrate (Sigma Aldrich), titanium (IV) iso-propoxide (98%, Strem Chemicals, Newburyport, MA, USA) and pluronic polymer F127 (Sigma Aldrich) were used to prepare the bioactive glass-coated titanium samples.
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5

Synthesis of Pyridine-based Compounds

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Silver nitrate, cobalt(II) nitrate, dimethylformamide, 2,6-pyridinedicarboxylic acid, ethanol, sodium hydroxide, Hydrazine hydrate, ethyl acetoacetate, aryl aldehydes, and barbituric acid derivatives were purchased from Sigma-Aldrich. The chemicals were used without further purification.
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6

Reagent Preparation for Analytical Studies

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Deionized water was used for the preparation and dilution of all solutions. The chemicals applied in this work were all of analytical grade. A Pb(II) stock solution of 1000 mg L−1 was prepared from the nitrate salt and diluted daily for designed experiments. α-Benzoin oxime, iron(III) chloride, phenol, sodium fluoride, sodium phosphate, nickel(II) nitrate, hexahydrate, sodium nitrate, lead(II) nitrate, cobalt(II) nitrate, zinc(II) nitrate, sodium carbonate, potassium chloride, magnesium nitrate hexahydrate, calcium chloride, sodium sulfate, and iron(II) nitrate were purchased from Sigma (St. Louis, MO, USA). Sodium hydroxide, hydrochloric acid, nitric acid, and hydrogen peroxide were purchased from Merck (Darmstadt, Germany).
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7

Evaluating Acrylamide Metabolism

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Acrylamide (AA) was obtained from Merck, Germany. rac-Glycidamide (GA) was obtained from Toronto Research Chemicals Inc, Canada. Hydroxocobalamin hydrochloride, cobalt(II) nitrate, sodium borohydride, rac-propylene oxide (PO), β-nicotinamide adenine dinucleotide phosphate sodium salt (NADP), glucose-6-phosphate, magnesium sulfate, potassium chloride, and trifluoroacetic acid were obtained from Sigma-Aldrich (Steinheim, Germany). Human and rat liver S9 fraction were purchased from Moltox (Boone, NC, USA); protein content was in the range 10–30 mg/mL, provided by the supplier for every batch. Human S9 was a pooled fraction of five smokers, all male. Rat S9 was from a male SD strain. All experiments concerning liver S9 were performed in accordance to relevant approval at Stockholm University. Stockholm University has obtained a general license from the Swedish Board of Agriculture for the import and use of animal products and by-products for research purposes. No experiments were performed on animals or on humans in this work. Only published in vivo data were used which are carefully cited.
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8

Cobalt-Zinc Complex Synthesis

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The essential chemicals used for the proposed experiment include cobalt II nitrate (Sigma Aldrich, New York, NY, USA), zinc II sulfate (Sigma Aldrich, New York, NY, USA), ammonia solution (25%) (Sigma Aldrich, New York, NY, USA), aspartic acid (Sigma Aldrich, New York, NY, USA), sodium dihydrogen phosphate, disodium hydrogen phosphate, potassium chloride, glucose, nitrite, melamine, glutamic acid, leucine, uric acid and ascorbic acid. All of the chemicals used for this study were of analytical grade and were used as bought without any further treatment.
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9

Hybrid Nanomaterial Synthesis and Characterization

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The AISI 1020 steel rod used in this study was obtained from scrap market in Minna, Niger State, Nigeria. Silver nitrate (AgNO3) at 99.8% purity, cobalt(II) nitrate, Co(NO3)2·6H2O, (97%), titanium(IV) isopropoxide (97%), carbon nanotubes (CNTs) (95%), concentrated hydrochloric acid (HCl) (90%), sodium hydroxide (NaOH) (90%) distilled water (95%), acetone (97%), aluminium chloride (99.5%), sodium acetate, sodium carbonate (Na2CO3), (95%), 10% Folin–Ciocalteau’s Reagent (95%) were obtained from sigma Aldrich, China and Kamel, England. Emery papers of 220, 400, 600 and 800 grits, magnetic stirrer, freeze drier, ultra-sonic bath, high speed refrigerated centrifuge, high resolution SEM.
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10

Synthesis of Cobalt-based Metal-Organic Framework

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Source chemicals including cobalt(ii) nitrate, pyridine 3,5-dicarboxylic acid, 1,2,3,4-cyclopentane tetracarboxylic acid were purchased from Sigma-Aldrich. All the precursors were of 99.99% purity and utilized without additional processing.
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