Db 5 gc column

Manufactured by Agilent Technologies

Sourced in United States

The DB-5 GC column is a high-performance capillary column designed for gas chromatography (GC) analysis. It features a 5% phenyl-methylpolysiloxane stationary phase, which provides excellent separation and resolution of a wide range of organic compounds, including hydrocarbons, alcohols, and halogenated compounds. The column is known for its durability, inertness, and stable performance across a wide temperature range.

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Lab products found in correlation

6890n network gas chromatograph, by Agilent Technologies (1 mentions) Agilent 5973 inert mass selective detector, by Agilent Technologies (1 mentions) Varian 9012 solvent delivery system, by Agilent Technologies (1 mentions) Gcms qp2020, by Shimadzu (1 mentions) Sep pak c 18 reverse phase cartridges, by Waters Corporation (1 mentions)

Close spelling variants of this product

DB-5 GC-MS column J&W GC DB-5 MS column DB-5 column

5 protocols using db 5 gc column

GC-MS Analysis of PAHs and OH-PAHs

Cited 3 times

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An Agilent 6890 GC chromatograph, equipped with 7683 series autosampler and 5973N mass selective detector with electron impact ionization was used for the analysis of 19 PAHs and 34 OH-PAHs. A DB-5 GC column (Agilent, 30 m x 25 m, 0.25 μm) was used and the carrier gas was helium with a flow rate of 1 ml/min. The GC oven temperature program for the analysis of PAH and OH-PAH was: 70 °C, hold for 1 min, then ramp 5 °C/min to 200 °C, hold for 5 min, ramp 5 °C/min to 250 °C, ramp 2 °C/min to 286 °C, and ramp 34 °C/min to 320 °C, hold for 5 min. The inlet and interface temperatures were 260 °C and 280°C, respectively. All analyses were done in selected ion monitoring (SIM) mode and Table S2 lists the m/z ions monitored. The total run time for the determination of19 PAHs and 34 OH-PAHs was 66 minutes. The method detection limits in urine and air were calculated based on response factors according to the US EPA method 8280A (U.S. EPA, 1996 ). Urinary PAH and OH-PAH concentrations were adjusted to creatinine concentration to account for dilution. The personal PM_2.5 samples were also analyzed for high molecular weight PAHs (302 MW) and oxy-PAHs using previously published methods (Jia et al., 2011 (link); Wang et al., 2012 (link); Wang et al., 2011 (link)).

Motorykin O., Schrlau J., Jia Y., Harper B., Harris S., Harding A., Stone D., Kile M., Sudakin D, & Massey Simonich S.L. (2014). Determination of Parent and Hydroxy PAHs in Personal PM2.5 and Urine Samples Collected During Native American Fish Smoking Activities. The Science of the total environment, 505, 694-703.

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Quantification of P450BM-3 Catalyzed Lauric Acid Oxidation

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Incubation mixtures (200 μL) contained P450_BM-3 (1 μM), lauric acid (1 mM), NADPH (1.25 mM), and 50 mM potassium MOPS buffer (pH 7.4). Reaction mixtures were incubated at room temperature for 10 min and terminated by the addition of 600 μL of cold ethyl acetate containing 0.1% HCO₂H (v/v), followed by mixing for 1 min with a vortex device and centrifugation at 3 × 10³ × g for 5 min. An aliquot of the organic layer was carefully removed and evaporated under a stream of nitrogen. Derivatization was carried out by adding 100 μL of N,O-bis(trimethyl)trifluoroacetamide (with 0.1% trichloromethylsilane, v/v) to the dried sample, followed by incubation at 70 °C for 60 min. The derivatizing reaction mixture was evaporated under a stream of nitrogen and reconstituted in hexane (50 μL). Product analysis was done using an Agilent GC/MS instrument with a 30 m × 0.25 mm × 0.25 μm DB-5 GC column (Agilent). Injection was (cold) on-column. The oven program used an initial temperature of 130 °C and was increased at 25 °C/min to 310 °C. Electron impact ionization was used, and ions in the range of 60–400 m/z were scanned at a rate of two scans per second. Negative control runs contained all of the components except NADPH.

Guengerich F.P, & Fekry M.I. (2020). Methylene Oxidation of Alkyl Sulfates by Cytochrome P450BM-3 and a Role for Conformational Selection in Substrate Recognition. ACS catalysis, 10(9), 5008-5022.

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NPLC/UV-Vis and GC/MS Analyses

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NPLC/UV-Vis was performed using a Varian 9012 Solvent Delivery System (Agilent, Santa Clara, CA) coupled to Jasco UV-1570 Intelligent UV/Vis Detector (Jasco, Easton, MD) using a Waters Spherisorb 5 μm NH₂ 10 × 250 mm semi-prep LC column (Waters, Milford, MA). GC/MS analyses were performed on an Agilent 6890N Network Gas Chromatograph coupled to Agilent 5973 Inert Mass Selective Detector (Agilent, Santa Clara, CA) using a Restek Rxi-PAH GC Column (Restek, Bellefonte, PA), 60 m length, 0.25 mm id, 0.10 μm film thickness, maximum programmable temperature 360 °C, and minimum bleed at 350 °C, and using an Agilent DB-5 GC Column (Agilent, Santa Clara, CA), 5% phenylmethylpolysiloxane stationary phase, 60 m length, 0.25 mm id, 0.25 μm film thickness, maximum programmable temperature 350 °C, and minimum bleed at 325 °C.

Oña-Ruales J.O., Sharma A.K, & Wise S.A. (2015). Identification and Quantification of Six-Ring C26H16 Cata-Condensed Polycyclic Aromatic Hydrocarbons in a Complex Mixture of Polycyclic Aromatic Hydrocarbons from Coal Tar. Analytical and bioanalytical chemistry, 407(30), 9165-9176.

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Headspace SPME-GC-MS for Volatile Aroma Analysis

Cited 1 time

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The relative content of volatile aroma compounds was determined by headspace solid phase microextraction gas chromatography-mass spectrometer (Agilent, Technologies, USA) (Zhang et al., 2023 (link)). 5 mL samples were taken in a 20 mL headspace flask and shaken at 50 °C for 15 min at a shaking speed of 250 rpm. Inserted the extraction needle into the headspace bottle to keep it at a distance of 1 cm from the liquor surface and extracted for 30 min until the distribution was balanced. After desorbing in GC injector at 220 °C for 5 min, aromas were separated on a DB-5 GC column (50 m × 0.25 mm × 0.25 μm, Agilent, USA) with helium carrier gas at a flow rate of 1.5 mL/min. An original temperature of 40 °C was held for 3 min, then risen to 240 °C at the rate of 3 °C /min and held for 10 min. And ion source temperature was 230 °C. Analyses were conducted with GC–MS QP2020 (Shimadzu, Kyoto, Japan), scanned in a mass acquisition range of 35 ∼ 750 m/z for quantitative analysis of detections.

Li P., Jia Y., Cai D., Wang X., Liu J., Zhu R., Wang Z., He Y, & Wen L. (2023). Study on the relationship between flavor components and quality of ice wine during freezing and brewing of 'beibinghong' grapes. Food Chemistry: X, 20, 101016.

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Quantifying Intestinal Ganglioside Content

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The total ganglioside content in the intestinal mucosa was determined by measuring the GG bound sialic acid with GC-MS. The GG were extracted and purified by using the Sep-Pak C18 reverse-phase cartridges (Waters, Milford, MA) according to the method of Schnabl et al. (2009) . The GG were derivatized by trimethylsilylation. One hundred-microliter ganglioside samples with 5 µg of phenyl N-acetyl-α-d-glucosaminide as internal standard were dried and treated with 700 µL of 0.05 N fresh methanolic HCl by heating for 1 h at 80°C. The mixture was cooled and extracted with 0.5 mL of hexane to remove the liberated fatty acid esters. The methanolic layer was dried under a nitrogen stream. The trimethylsilylation derivatization reagent was formed according to the method of Carter and Gaver (1967) . The silylation reagent (50 µL) was added to the samples in the small ample vials. The samples were vortexed and allowed to stand for 15 min. The deriva-tized samples were transferred into GC inserts and 1 µL was injected per assay into a DB-5 GC Column (Agilent, Santa Clara, CA) installed on a Shimadzu GC (Shimadzu, Colombia, MD) coupled with a MS. The quantification was achieved from the standard curve generated by concurrent analysis of a series of ganglioside GD3 standards in different concentrations.

Zhou A.L, & Ward R.E. (2019). Milk polar lipids modulate lipid metabolism, gut permeability, and systemic inflammation in high-fat-fed C57BL/6J ob/ob mice, a model of severe obesity. Journal of dairy science, 102(6).

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