All the data collected for subjects 1–8 were made using a wired data acquisition system. The resistance from the strain sensor was measured using a Wheatstone bridge configuration with 4.7 kΩ resistors. The differential potential was measured using a multifunction data acquisition system (National Instruments, USB-6003). A continuous spirometer (Vyaire, Vmax Encore 229) was used to measure respiration airflow from the patient; the data was outputted as voltage from the Vmax Encore and measured using the multifunction data acquisition system. A triple axis accelerometer (Adafruit, ADXL326) was connected to a second data acquisition system to measure movement of the subject. The digital acquisition system was connected to the computer via USB, and all data was recorded and timestamped using Signal Express (National Instrument, Signal Express 2015) at a sampling frequency of 1000 Hz. Supplemental Fig. 1 shows the sensor placement on the body and hardware setup.
Signal express
Manufactured by National Instruments
Signal Express is a data acquisition and analysis software tool developed by National Instruments. It provides a simple and intuitive interface for configuring and controlling a range of data acquisition hardware, allowing users to acquire, analyze, and visualize data from various sources.
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4 protocols using signal express
1
Multimodal Respiratory and Movement Monitoring
2
Synchronous Video-EEG Recordings in Mice
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Synchronous video-EEG recordings were carried out at least 7 days after surgery in freely moving mice in an 8-inch diameter recording chamber. Field potential recordings were bandpass filtered and amplified (0.3 Hz – 1 kHz, gain: 1000, Model 3500, A-M Systems, Carlsborg, WA), digitized on-line at a rate of 2048 s−1 (PCI-MIO16E-4 data acquisition board National Instruments, Austin, TX), and acquired continuously (SignalExpress, National Instruments, Austin, TX). All data analysis was performed under IGOR Pro v6.22A (Lake Oswego, OR). Morlet wavelet analysis was performed for time-frequency analysis of local field potential recordings.
3
Graphene Electrodes for Electrical Potential Recording
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The electrical potential was recorded by two identical graphene electrodes of the array. From the copper wires, the signal was delivered to a differential AC amplifier (A-M Systems, 1,700) at a 20-kHz sampling rate, where it received a × 100 gain and band-pass filter between 300 Hz and 5 kHz. Following amplification, the signal was processed using data acquisition board (NI, BNC-2110) and software (NI, Signal Express).
4
Synchronous Video-EEG Recordings in Mice
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