To acquire highly magnified images, field-emission scanning electron microscope (FEI Quanta 400, Houston, Texas, USA) was adopted to investigate the microstructure of electrospun nanofiber conduits and nerve tissues. It was operated at a working distance of 3 mm, an acceleration voltage of 15 kV and a beam current of 1.6 × 10−6 A. The specimens were made conductive by depositing a 2 nm-thick layer of platinum using a Cressington Sputter Coater 108A operated at a working distance of 100 mm and a current of 20 mA. Photographs were captured for each section used in the final quantitative analysis (Shen et al., 2011). To measure the sizes of the nerve and nanofibers, 30–50% of the sciatic nerve section area was randomly selected from each nerve specimen using Image-Pro Plus (Media Cybernetics, Rockville, MD, USA).
Sputter coater 108a
Manufactured by Cressington
Sourced in United Kingdom
The Sputter Coater 108A is a vacuum-based deposition system designed for the application of thin, uniform metallic or ceramic coatings onto various substrates. It utilizes a sputtering process to deposit the coating material onto the target surface.
Automatically generated - may contain errors
Lab products found in correlation
Jsm 6360 sem, by JEOL (2 mentions)
Quanta 400, by Thermo Fisher Scientific (1 mentions)
Image pro plus, by Media Cybernetics (1 mentions)
Labram hr evolution raman spectrometer, by Horiba (1 mentions)
Q500 equipment, by TA Instruments (1 mentions)
Jsm 6010lv microscope, by JEOL (1 mentions)
Jsm 6360, by JEOL (1 mentions)
6 protocols using sputter coater 108a
1
Microscopic Analysis of Electrospun Nanofiber Conduits
2
Thermal and Spectroscopic Analysis of Functionalized Graphene
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thermogravimetric analysis (TGA) was performed on Q500 equipment (TA Instruments®, New Castle, DE, USA). EG, f-EG, and [(f-EG)+Ag] were placed in a platinum crucible and heated from 40–800 °C at 10 °C min−1 under a nitrogen atmosphere of 50 mL min‒1.
Raman spectra were acquired using a LabRAM HR Evolution Raman spectrometer with a microscope (Horiba Scientific, Piscataway, NJ, USA) using a laser with a wavelength of 532 nm and a grating of 600 gr mm‒1. The results were analyzed with the Horiba Scientific’s Labspec 6 (version 6.4.4) Spectroscopy Suite Software (Horiba France SAS, Longjumeau, France) and the peak positions were determined by applying a baseline (in LabSpec 6).
Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were carried out using a FEI Nova 200 FEG-SEM/EDS (FEI Europe Company, Hillsboro, OR, USA). The samples were previously sputtered with a gold layer, using a sputter coater 108A (Cressington, Watford, UK).
Raman spectra were acquired using a LabRAM HR Evolution Raman spectrometer with a microscope (Horiba Scientific, Piscataway, NJ, USA) using a laser with a wavelength of 532 nm and a grating of 600 gr mm‒1. The results were analyzed with the Horiba Scientific’s Labspec 6 (version 6.4.4) Spectroscopy Suite Software (Horiba France SAS, Longjumeau, France) and the peak positions were determined by applying a baseline (in LabSpec 6).
Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were carried out using a FEI Nova 200 FEG-SEM/EDS (FEI Europe Company, Hillsboro, OR, USA). The samples were previously sputtered with a gold layer, using a sputter coater 108A (Cressington, Watford, UK).
3
Morphological Analysis of Multilayered Films
4
Scanning Electron Microscopy of Biofilm
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The biofilm formed on the 35 mm polystyrene dishes was also examined using scanning electron microscopy (SEM) using a modification of a previously described method [13 (link)]. The biofilm formed on the dishes was rinsed with distilled water and fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) at 4°C for 24 h. After sequential dehydration with graded concentrations of ethanol (60, 70, 80, 90, 95, and 100%), the samples were freeze-dried, sputter-coated with gold (108A sputter coater, Cressington Scientific Instruments Inc., Watford, England, UK), and observed using a scanning electron microscope (JSM-6360 SEM, JEOL, Tokyo, Japan).
5
Biofilm Assay for Antimicrobial Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The biofilm assay was based on a previously described method [13 (link), 14 (link)]. Biofilm formation was measured by staining with safranin and observed by scanning electron microscopy (SEM). C. obtusa essential oil was added to BHI broth containing 0.1% sucrose in 35 mm polystyrene dishes. The cultures were then inoculated with a seed culture of S. mutans (final: 5 × 105 CFU/mL) and incubated for 24 h at 37°C. After incubation, the supernatants were removed, and the dishes were rinsed with distilled water. Biofilm formation were stained with 0.1% safranin and photographed. The bound safranin was released from the stained cells with 30% acetic acid and the absorbance of the solution was measured at 530 nm. In addition, biofilm on 35 mm polystyrene dishes was observed by SEM [15 (link)]. The biofilm formed on the dishes was rinsed with distilled water, fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) at 4°C for 24 h, and dehydrated with ethanol gradient series (60%, 70%, 80%, 90%, 95%, and 100%). Then, the samples were freeze-dried, sputter-coated with gold (108A sputter coater; Cressington Scientific Instruments, Inc., Watford, England, United Kingdom), and observed by SEM (JSM-6360, JEOL, Tokyo, Japan).
6
Scanning Electron Microscopy of Biofilms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The biofilm on 35 mm polystyrene dishes was also determined by SEM using a modification of a previously described method [4 (link)]. The biofilm formed on the dishes was rinsed with distilled water and fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) at 4°C for 24 h. After gradual dehydration with ethyl alcohol 60, 70, 80, 90, 95, and 100%, the sample was freeze-dried. The specimens were then sputter-coated with gold (108A sputter coater, Cressington Scientific Instruments Inc., Watford, UK). For observation, a JSM-6360 SEM (JEOL, Tokyo, Japan) was used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!