Equinox 55 infrared microscope

Optical rotations were carried out on an MCP-200 polarimeter (Anton Paar, Graz, Austria) with MeOH as solvent at 25 °C. UV spectra were acquired on a Blue Star A spectrophotometer. IR data were performed on a Fourier transformation infrared spectrometer coupled with an EQUINOX 55 infrared microscope (Bruker, Fällanden, Switzerland). Here, 1D and 2D NMR spectra were tested on a Bruker Avance 400 MHz spectrometer (Bruker, Fällanden, Switzerland) using TMS as an internal standard. HRESIMS and ESIMS data were recorded on an LTQ-Orbitrap LC-MS spectrometer (Thermo Corporation, MA, USA) and an ACQUITY QDA (Waters Corporation, MA, USA), respectively. HPLC preparative separations were performed on a Shimadzu Essentia LC-16. The Welch-Ultimate XB-C18 column (250 × 21.2 mm, 5 μm, 12 nm, Welch Materials, Inc., Shanghai, China) was used for preparative HPLC. Semi-preparative HPLC separations were performed on ACE-5-C18-AR and ACE-5-CN-ES columns (250 × 10 mm, 5 μm, 12 nm, Advanced Chromatography Technologies Ltd., Guangzhou, China). The silica gel (200−300 mesh, Qingdao Marine Chemical Inc., Qingdao, China) and Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) were subjected to column chromatography (CC).

Optical rotations were carried out on an MCP-200 polarimeter (Anton Paar, Graz, Austria) with MeOH as solvent at 25 °C. UV spectra were acquired on a Blue Star A spectrophotometer. IR data were performed on a Fourier transformation infrared spectrometer coupled with an EQUINOX 55 infrared microscope (Bruker, Fällanden, Switzerland). Here, 1D and 2D NMR spectra were tested on a Bruker Avance 400 MHz spectrometer (Bruker, Fällanden, Switzerland) using TMS as an internal standard. HR-ESIMS and ESIMS data were recorded on an LTQ-Orbitrap LC-MS spectrometer (Thermo Corporation, Waltham, MA, USA) and an ACQUITY QDA (Waters Corporation, Milford, MA, USA), respectively. HPLC preparative separations were performed on a Shimadzu Essentia LC-16. The Welch-Ultimate XB-C18 column (250 × 21.2 mm, 5 μm, 12 nm, Welch Materials, Inc., Shanghai, China) was used for preparative HPLC. Semi-preparative HPLC separations were performed on ACE-5-C18-AR and ACE-5-CN-ES columns (250 × 10 mm, 5 μm, 12 nm, Advanced Chromatography Technologies Ltd., Guangzhou, China). The silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China) and Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden) were subjected to column chromatography (CC).

Optical rotations were recorded on an MCP-200 polarimeter (Anton Paar, Graz, Austria) with MeOH as solvent at 25 °C. UV spectra were measured on a Blue Star A spectrophotometer. IR data were carried out on a Fourier transformation infrared spectrometer coupled with an EQUINOX 55 infrared microscope (Bruker, Rheinstetten, Germany). A Bruker Avance 400 MHz spectrometer (Bruker, Karlsruhe, Germany) was used for 1D and 2D NMR spectra test with TMS as an internal standard. ESIMS and HRESIMS data were measured on an ACQUITY QDA (Waters Corporation, Milford, MA, USA) and an LTQ-Orbitrap LC-MS spectrometer (Thermo Corporation, Waltham, MA, USA), respectively. A Shimadzu Essentia LC-16 was used for HPLC preparative separations by a Welch-Ultimate XB-C18 column (250 × 21.2 mm, 5 μM, 12 nm, Welch Materials, Inc., Shanghai, China) an ACE-5-C18-AR, ACE-5-CN-ES and ACE-C18-PFP column (250 × 10 mm, 5 μM, 12 nm, FLM Advanced Chromatography Technologies Ltd., Guangzhou, China). Column chromatography (CC) was performed on silica gel (200−300 mesh, Qingdao Marine Chemical Inc., Qingdao, China) and Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden).